Increase of proton electrochemical potential and ATP synthesis in rat liver mitochondria irradiated in vitro by helium‐neon laser

Passarella, Salvatore; Casamassima, E.; Molinari, S.; Pastore, Donato; Quagliariello, E; Catalano, I. M.; Cingolani, A.

doi:10.1016/0014-5793(84)80577-3

Cited by 460 publications

(260 citation statements)

References 10 publications

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“…Our previous study, in line with others, have shown that LLL illumination can sustain mitochondrial functions in cells under various conditions of stress, including enhancement of ATP synthesis, suppression of ROS production, and reduction of apoptosis. [26][27][28] The ability of sustaining mitochondrial functions in the injured brain may account for the benefit of LLL in TBI management showed recently. 12,17,28 The current investigations further validate that LLL stimulates ATP production and suppresses ROS generation in hypoxic cells and brain tissue.…”

Section: Discussionmentioning

confidence: 99%

Low-Level Light in Combination with Metabolic Modulators for Effective Therapy of Injured Brain

Dong

Zhang

Hamblin

et al. 2015

J Cereb Blood Flow Metab

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Vascular damage occurs frequently at the injured brain causing hypoxia and is associated with poor outcomes in the clinics. We found high levels of glycolysis, reduced adenosine triphosphate generation, and increased formation of reactive oxygen species and apoptosis in neurons under hypoxia. Strikingly, these adverse events were reversed significantly by noninvasive exposure of injured brain to low-level light (LLL). Low-level light illumination sustained the mitochondrial membrane potential, constrained cytochrome c leakage in hypoxic cells, and protected them from apoptosis, underscoring a unique property of LLL. The effect of LLL was further bolstered by combination with metabolic substrates such as pyruvate or lactate both in vivo and in vitro. The combinational treatment retained memory and learning activities of injured mice to a normal level, whereas other treatment displayed partial or severe deficiency in these cognitive functions. In accordance with well-protected learning and memory function, the hippocampal region primarily responsible for learning and memory was completely protected by combination treatment, in marked contrast to the severe loss of hippocampal tissue because of secondary damage in control mice. These data clearly suggest that energy metabolic modulators can additively or synergistically enhance the therapeutic effect of LLL in energyproducing insufficient tissue-like injured brain.

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Section: Discussionmentioning

confidence: 99%

Low-Level Light in Combination with Metabolic Modulators for Effective Therapy of Injured Brain

Dong

Zhang

Hamblin

et al. 2015

J Cereb Blood Flow Metab

View full text Add to dashboard Cite

show abstract

“…[9][10][11] This absorbed energy is transformed into ATP that increases the activity of the mitochondrial proton pump, increasing oxygen consumption and enhancing synthesis of nicotinamide adenine dinucleotide dehydrogenase (NADH), RNA and protein. [12][13][14] Finally, these changes will lead to increased expression of growth factors and cell proliferation. 18,19 Furthermore, the most important intracellular organelle that produces reactive oxygen species (ROS) is the mitochondria.…”

Section: Introductionmentioning

confidence: 99%

A Comparative Study of 660 nm Low-Level Laser and Light Emitted Diode in Proliferative Effects of Fibroblast Cells

et al. 2017

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Introduction: In recent years, low-power lasers have been widely used in medicine. With the introduction of affordable light emitted diode (LED), clinical application of LED light has become more and more popular. However, some researchers believe that due to lack of coherence of the LED light, it can be different in terms of biological effects, in comparison with laser. In this study, the biological effects of low-level laser (LLL) to those of LED light are compared and discussed. Methods: Human skin fibroblast cell line Hu02 was irradiated with LLL and LED light with a wavelength of 660 nm, power output of 35 mW and in continuous mode and the control group was not irradiated. The biological effects were compared through analysis of cell proliferation, production of reactive oxygen species (ROS) within the cell and rate of cell division. Results: Our findings showed that production of ROS within the cell was linearly increased both in the LED and laser light irradiated cells. However, laser light is more incremental in comparison to LED light. The MTT results showed that laser light at low energy density (less than 5 J/cm 2 ) increased the rate of cell proliferation after 24 hours. Although, the rate of cell division was increased in energy density of 1 J/cm 2 compared to the control group, this increase was not statistically significant. Discussion: The findings indicated that the coherence properties of laser light provided more energy for the cells, and in a constant energy density, laser light created more oxidative stresses in comparison with LED light.

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“…Karu [4] reported several years ago that light therapy effects on biological tissues can be classified as either primary (light-tissue interactions) or secondary mechanisms (photobiomodulation). One of the most frequently observed effects of photobiomodulation is the increased ATP synthesis in cultured cells [4,13,14].…”

Section: Introductionmentioning

confidence: 99%

Time response of increases in ATP and muscle resistance to fatigue after low-level laser (light) therapy (LLLT) in mice

et al. 2015

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Recently, low-level laser (light) therapy has been used to increase muscle performance in intense exercises. However, there is a lack of understanding of the time response of muscles to light therapy. The first purpose of this study was to determine the time response for light-emitting diode therapy (LEDT)-mediated increase in adenosine triphosphate (ATP) in the soleus and gastrocnemius muscles in mice. Second purpose was to test whether LEDT can increase the resistance of muscles to fatigue during intense exercise. Fifty male Balb/c mice were randomly allocated into two equal groups: LEDT-ATP and LEDT-fatigue. Both groups were subdivided into five equal subgroups: LEDT-sham, LEDT-5 min, LEDT-3 h, LEDT-6 h, and LEDT-24 h. Each subgroup was analyzed for muscle ATP content or fatigue at specified time after LEDT. The fatigue test was performed by mice repeatedly climbing an inclined ladder bearing a load of 150 % of body weight until exhaustion. LEDT used a cluster of LEDs with 20 red (630± 10 nm, 25 mW) and 20 infrared (850±20 nm, 50 mW) delivering 80 mW/cm 2 for 90 s (7.2 J/cm 2 ) applied to legs, gluteus, and lower back muscles. LEDT-6 h was the subgroup with the highest ATP content in soleus and gastrocnemius compared to all subgroups (P<0.001). In addition, mice in LEDT-6 h group performed more repetitions in the fatigue test (P<0.001) compared to all subgroups: LEDT-sham and LEDT-5 min (~600 %), LEDT-3 h (~200 %), and LEDT-24 h (~300 %). A high correlation between the fatigue test repetitions and the ATP content in soleus (r=0.84) and gastrocnemius (r=0.94) muscles was observed. LEDT increased ATP content in muscles and fatigue resistance in mice with a peak at 6 h. Although the time response in mice and humans is not the same, athletes might consider applying LEDT at 6 h before competition.

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Increase of proton electrochemical potential and ATP synthesis in rat liver mitochondria irradiated in vitro by helium‐neon laser

Abstract: not received MitochondriaLaser Transmembrane proton gradient Membrane potential A TP synthesis Oxygen uptake

Cited by 460 publications

References 10 publications

Low-Level Light in Combination with Metabolic Modulators for Effective Therapy of Injured Brain

Low-Level Light in Combination with Metabolic Modulators for Effective Therapy of Injured Brain

A Comparative Study of 660 nm Low-Level Laser and Light Emitted Diode in Proliferative Effects of Fibroblast Cells

Time response of increases in ATP and muscle resistance to fatigue after low-level laser (light) therapy (LLLT) in mice

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