Increase of Reaction Rate and Sensitivity of Low-Abundance Enzyme Assay Using Micro/Nanofluidic Preconcentration Chip

Lee, Jeong Hoon; Song, Yong-Ak; Tannenbaum, Steven R.; Han, Jongyoon

doi:10.1021/ac800362e

Cited by 95 publications

(94 citation statements)

References 27 publications

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“…9). 35 By using a micro/nanofluidic preconcentration chip and concentrating a mixture of trypsin as enzyme and BODIPY Fl casein as the fluorogenic substrate, the reaction time required to turn over substrates at 1 ng mL −1 was only ~10 min compared to ~1 h without preconcentration. Furthermore, trypsin activity could be measured down to a concentration level of 10 pg mL −1 , which is an ~100-fold enhancement in sensitivity compared to the result without preconcentration.…”

Section: Applicationsmentioning

confidence: 99%

Nanofluidic concentration devices for biomolecules utilizing ion concentration polarization: theory, fabrication, and applications

2010

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Recently, a new type of electrokinetic concentration devices has been developed in a microfluidic chip format, which allows efficient trapping and concentration of biomolecules by utilizing ion concentration polarization near nanofluidic structures. These devices have drawn much attention not only due to their potential application in biomolecule sensing, but also due to the rich scientific content related to ion concentration polarization, the underlying physical phenomenon for the operation of these electrokinetic concentration devices. This tutorial review provides an introduction to the scientific and engineering advances achieved, in-depth discussion about several interesting applications of these unique concentration devices, and their current limitations and challenges.

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Section: Applicationsmentioning

confidence: 99%

Nanofluidic concentration devices for biomolecules utilizing ion concentration polarization: theory, fabrication, and applications

2010

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“…In the depletion mode, the depletion region is formed due to the concentration polarization and then in the trapping mode, the molecules driven with an electroosmotic flow are electrokinetically trapped in front of the depletion region, resulting in increased concentration (Lee et al 2008b) across the nanochannel array (Kim et al 2008b). The working principle of this device was reported by Kirby and Hasselbrink (2004a).…”

Section: Other Applicationsmentioning

confidence: 99%

Ion bridges in microfluidic systems

Park

Chung

Kim

2009

Microfluid Nanofluid

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Recently many microfluidic systems are increasingly equipped with functional units for ionic controls for various applications. In this review article, we define an ion bridge as a structure that controls current or distribution of ions in a microfluidic system, and summarize the ion bridges in the literature in terms of characteristics, fabrication methods, advantages and disadvantages. The ion bridges play two basic roles, namely to ensure electrical contact in a microfluidic network and mechanically separate a liquid phase from another. More interestingly, the charged surfaces of ion bridges, which can be chemically modified, create new characteristics such as permselectivity and concentration polarization. Asymmetric ion transport as well as ionic conductivity through the ion bridges suggests a variety of applications including sample preconcentration, electroosmotic pump, electrospray ionization, electrically driven valve and many others. This review categorizes the ion bridges into several classes and describes the structures, materials, fundamental functions and applications. In Perspectives, new opportunities of microfluidics and nanofluidics provided by the ion bridges are discussed.

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“…Employing micro/nanofluidic networks, the ICP phenomena are frequently utilized to preconcentrate charged sample analytes [3,7,8]. Han et al have investigated ICP preconcentration of various biomolecules in fluidic systems [9][10][11]. Moreover, desalination of seawater using ICP phenomena has been reported [12,13].…”

Section: Introductionmentioning

confidence: 99%

Folding-paper-based preconcentrator for low dispersion of preconcentration plug

Lee

Yoo

Han

et al. 2017

Micro and Nano Syst Lett

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Ion concentration polarization (ICP) has been widely studied for collecting target analytes as it is a powerful preconcentrator method employed for charged molecules. Although the method is quite robust, simple, cheap, and yields a high preconcentration factor, a major hurdle to be addressed is extracting the preconcentrated samples without dispersing the plug. This study investigates a 3D folding-paper-based ICP preconcentrator for preconcentrated plug extraction without the dispersion effect. The ICP preconcentrator is printed on a cellulose paper with pre-patterned hydrophobic wax. To extract and isolate the preconcentration plug with minimal dispersion, a 3D pop-up structure is fabricated via water drain, and a preconcentration factor of 300-fold for 10 min is achieved. By optimizing factors such as the electric field, water drain, and sample volume, the technique was enhanced by facilitating sample preconcentration and isolation, thereby providing the possibility for extensive applications in analytical devices such as lateral flow assays and FTA R cards.

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Increase of Reaction Rate and Sensitivity of Low-Abundance Enzyme Assay Using Micro/Nanofluidic Preconcentration Chip

Cited by 95 publications

References 27 publications

Nanofluidic concentration devices for biomolecules utilizing ion concentration polarization: theory, fabrication, and applications

Nanofluidic concentration devices for biomolecules utilizing ion concentration polarization: theory, fabrication, and applications

Ion bridges in microfluidic systems

Folding-paper-based preconcentrator for low dispersion of preconcentration plug

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