Increased 8-Isoprostane, a Marker of Oxidative Stress, in Exhaled Condensate of Asthma Patients

Abstract

Oxidative stress has an important role in the pathogenesis of asthma. 8-Isoprostane is a prostaglandin (PG)-F2-like compound belonging to the F2 isoprostane class that is produced in vivo by the free radical-catalyzed peroxidation of arachidonic acid. 8-Isoprostane is a biomarker of oxidative stress, and its concentration is increased in the bronchoalveolar lavage fluid of patients with interstitial lung diseases. We measured 8-isoprostane concentrations in exhaled breath condensate in healthy subjects and in patients with mild (steroid naive, n = 12), moderate (inhaled steroid treatment, n = 17), and severe asthma (oral steroid treatment, n = 15). We also measured exhaled carbon monoxide (CO) and nitric oxide (NO), which may also reflect oxidative stress in the airways. 8-Isoprostane was detectable in breath condensate of normal subjects (15.8 +/- 1.6 pg/ml), and was increased in the breath condensate of patients with mild (33.7 +/- 2.8, p < 0.001), moderate (38.3 +/- 3.7 pg/ml, p < 0. 001), and severe asthma (48.9 +/- 5.0 pg/ml, p < 0.001). There was a positive correlation (r = 0.68, p < 0.05) of 8-isoprostane with NO, but not with CO, in the exhaled air of patients with mild asthma, but not in that of patients with moderate or severe asthma. There was no correlation between 8-isoprostane and lung function tests in any group of patients. Our study shows that oxidative stress is increased in asthmatic subjects as reflected by 8-isoprostane concentrations in breath condensate.

Magnesium and calcium in exhaled breath condensate of children with asthma and gastroesophageal reflux disease

“…Biomarkers are originated from lower airways, but there is the possibility of saliva, upper respiratory tract contamination. To date, changes of biochemical markers of inflammation and oxidative stress have been most extensively investigated in patients with asthma (38,39), COPD (40,41), cystic fibrosis (42,43), and lung carcinoma (44) and less extensively in other pulmonary diseases (45). There is a general opinion that profiles of different analytes in EBC could be chosen in the future to help in the differential diagnosis of pulmonary diseases (16).…”

Corticosteroid effects on cell signalling

“…Oxidative stress is also increased in patients with severe asthma and during exacerbations [99][100][101], such that a reduction in HDAC may also account for the reduced responsiveness to corticosteroids in these patients and the relative unresponsiveness of acute exacerbation of asthma to corticosteroids.…”

Vitamin C in breast milk may reduce the risk of atopy in the infant

“…Firstly, the inflammatory process results in the generation of oxygen free radicals, and antioxidant defence systems are important in controlling inflammation (Greene, 1999). Atopic diseases, in particular asthma and atopic dermatitis, are characterized by increased oxidative stress (Montuschi et al, 1999;Omata et al, 2001). Second, there is evidence that a low antioxidant intake may be associated with allergic symptoms; low vitamin C intake has been linked with wheezing (Bodner et al, 1999) and bronchial hyper-reactivity (Soutar et al, 1997) and an inverse association has been observed between dietary vitamin E intake and serum total IgE .…”