Increased ability of transgenic plants expressing the bacterial enzyme ACC deaminase to accumulate Cd, Co, Cu, Ni, Pb, and Zn

Evidence for the involvement of ACC deaminase from Pseudomonas putida UW4 in the biocontrol of pine wilt disease caused by Bursaphelenchus xylophilus

“…These results suggest that ACC deaminase activity, and the consequent lowering of deleterious plant ethylene levels, is the mechanism responsible for P. putida UW4 plant growth promotion activity as well as its ability to protect plants against biotic as well as abiotic stress (Grichko et al 2000;Wang et al 2000;Cheng et al 2007;Glick et al 2007;Hao et al 2007;Gamalero et al 2010;Toklikishvili et al 2010). In addition, it has been previously demonstrated that transgenic plants that express a bacterial acdS gene under the control of a root specific promoter are more resistant to pathogen induced stress as well as abiotic stress caused by salt, flooding and metals (Robison et al 2001a, b;Grichko and Glick 2001;Grichko et al 2000;Stearns et al 2005;Sergeeva et al 2006).…”

“…This enzyme cleaves the ethylene precursor, ACC, into ammonia and a-ketobutyrate (Honma and Shimomura 1978) and thereby prevents ethylene levels in plants from rising to deleterious levels (Glick et al 1998. The ACC deaminase-producing bacterium Pseudomonas putida UW4 has been shown to be an effective PGPB protecting several different plant hosts from a variety of stress conditions including flooding, salt, pathogens and metals (Grichko et al 2000;Wang et al 2000;Grichko and Glick 2001;Cheng et al 2007;Hao et al 2007;Gamalero et al 2010;Toklikishvili et al 2010).…”

Rhizosphere colonization and arsenic translocation in sunflower (Helianthus annuus L.) by arsenate reducing Alcaligenes sp. strain Dhal-L

“…Bacterial cell suspension was prepared by growing Alcaligenes sp. strain Dhal-L in 200 mL DF medium containing 3 mmol L -1 ACC, as the ACCD is an inducible enzyme (Grichko et al 2000). The cells were harvested by centrifugation (11,0009g for 30 min) and suspended in CaSO 4 until final OD (600 nm) of 0.35 was achieved (i.e., about 10 8 CFU mL -1 ).…”

“…Some plants respond to biological-and environmental-stresses by synthesizing ''stress'' ethylene from the precursor 1-amino-cyclopropane-1-carboxylic acid (ACC) (Morgan and Drew 1997). Plant growth-promoting (PGP) bacteria that produce the enzyme ACC deaminase cleave ACC and lower the level of stress-induced ethylene, facilitating the formation of longer roots in plants growing in the presence of heavy metals (Grichko et al 2000), thus causing an enhanced uptake of inorganic contaminants through modification of root architecture and of root uptake system of plant (Arshad et al 2007). Recently it was demonstrated that the presence of arsenic-resistant bacteria (ARB) in rhizosphere influence arsenic content in plants (Xiong et al 2010).…”

New advances in plant growth-promoting rhizobacteria for bioremediation

“…Besides transition of gene between bacteria, transgenic plants have been constructed for higher remediation efficiency (Grckho et al, 2000;Nie et al, 2002;Stearns et al, 2005). The expression of ACC deaminase in the plant exhibits several advantages against in the bacteria: (1) during the initial stages of seed germination, the bacterial ACC deaminase activity is likely to be much lower than the activity in transgenic plants (Nie et al, 2002); (2) it can constantly stimulate plant growth, which leads to a higher metal accumulation; (3) in some cases an increase in the shoot/root ratio (Grckho et al, 2000); (4) prompting metal uptake of certain fast-growing plants for the substitution of slow-growing hyperaccumulators (Stearns et al, 2005).…”