Increased accumulation of paclitaxel and doxorubicin in proliferating capillary cells and prostate cancer cells following ultrasound exposure

Jackson, John K.; Pirmoradi, Fatemeh Nazly; Wan, Chung-Ping Leon; Siu, Tung; Chiao, Mu; Burt, Helen M.

doi:10.1016/j.ultras.2011.05.008

Cited by 22 publications

(12 citation statements)

References 35 publications

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“…In the present study, different concentrations of SonoVue™ microbubbles were used combined with 45 kHz 0.3 Wcm 2 ultrasound to examine the effects on apoptosis of VSMCs. Clinically, ultrasound is described as being of low, medium or high intensity at values of 0-0.5, 0.5-3 and >3 Wcm 2 , respectively (20). As a result of the high energies involved in ultrasound treatment, cell lysis is the major result and it is possible that other effects on the surviving cells are masked (21,22).…”

Section: Discussionmentioning

confidence: 99%

“…Different settings of frequency and energy density in ultrasonic treatment procedures lead to different impacts on cell proliferation, morphology and differentiation, and may be used to stimulate or inhibit the growth of individual cell types (23). In previous studies, ultrasound of 1 MHz or >1 MHz was usually used by a combination of microbubbles, which induced apoptosis (20,24). Ultrasound frequencies markedly <1 MHz, in particular ~45 kHz, have not been widely studied (11).…”

Section: Discussionmentioning

confidence: 99%

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Low-frequency low energy ultrasound combined with microbubbles induces distinct apoptosis of A7r5 cells

Zhang

Zhou

Cheng

et al. 2014

Molecular Medicine Reports

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The present study aimed to investigate whether low frequency low energy ultrasound combined with microbubbles induces apoptotic cell death of A7r5 rat aortic vascular smooth muscle cells, and to identify the possible mechanisms underlying this effect. Ultrasonic waves (45 kHz with 0.3 Wcm2 of intensity for 0, 10, 20 and 30 sec) were used together with different dosages of SonoVue™ microbubbles (0, 14, 28, 42 and 56 µl), respectively. The cell viability and apoptotic rate were determined by trypan blue staining immediately following treatment and flow cytometry 24 h thereafter. The treatment conditions resulting in the lowest amount of necrosis, highest apoptotic rate and lowest microbubble dosage was selected for the US+MB group, which was treated with ultrasound combined with microbubbles. The cell proliferation 24 h following treatment was determined and western blot analysis was applied to examine the expression of apoptosis‑associated proteins, B-cell lymphoma 2 (Bcl‑2) and Bcl-2-associated X (Bax). The harmonic acoustic pressure amplitude was measured to obtain the cavitation intensity. The combination of 20 sec ultrasound irradiation and 14 µl SonoVue™ was selected as the treatment conditions for the US+MB group. The results demonstrated that both ultrasound alone (the US group) and in combination with microbubbles significantly inhibited the proliferation of A7r5 cells compared with that of the control (P<0.01), and the suppression in the US+MB group was significantly greater (P<0.01). The apoptotic rate in A7r5 cells induced by this combination treatment (16.62±0.93%) was significantly higher than that in the control (3.93±0.39%; P<0.01) and US (6.88±1.87%; P<0.01) groups. Treatment with ultrasound combined with microbubbles increased the expression of Bax and decreased the ratio of Bcl‑2/Bax compared with those in the control and US groups. The cavitation induced by ultrasound combined with microbubble treatment was more intense than that by ultrasound alone. The results demonstrated that the cell death and apoptosis of A7r5 cells were associated with ultrasound duration and microbubble dosage. Low frequency ultrasound combined with microbubbles induced apoptosis in A7r5 cells through the upregulation of Bax and the downregulation of the Bcl‑2/Bax ratio, where the cavitation effect may have an important role.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Low-frequency low energy ultrasound combined with microbubbles induces distinct apoptosis of A7r5 cells

Zhang

Zhou

Cheng

et al. 2014

Molecular Medicine Reports

View full text Add to dashboard Cite

show abstract

“…Moreover, the apoptosis effect induced in tumor by ultrasound can be enhanced by porphyrins, anticancer drugs and microbubbles [12,13]. Clinically, ultrasound is described as being of low, me-dium or high intensity at values of 0-0.5, 0.5-3 and above 3 W/cm 2 , respectively [14]. In the past, in studies involving ultrasound combined with microbubbles that demonstrated induced apoptosis in cancer cells, the authors usually used ultrasound frequencies between 1 and 4 MHz [7,14].…”

mentioning

confidence: 99%

“…Clinically, ultrasound is described as being of low, me-dium or high intensity at values of 0-0.5, 0.5-3 and above 3 W/cm 2 , respectively [14]. In the past, in studies involving ultrasound combined with microbubbles that demonstrated induced apoptosis in cancer cells, the authors usually used ultrasound frequencies between 1 and 4 MHz [7,14]. As a result of the high energies involved in ultrasound treatment, cell lysis is the major phenomenon, and probably masks other effects on the surviving cells [15,16].…”

mentioning

confidence: 99%

Treatment of PC-3 cells with ultrasound combined with microbubbles induces distinct alterations in the expression of Bcl-2 and Bax

et al. 2013

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“…Na terapia anticâncer, a administração sistêmica de drogas quimioterápicas resulta na exposição de todos os tecidos em diferentes concentrações de drogas citotóxicas. Portanto, há uma necessidade de fornecer regimes de dose suficiente, constantes e em faixas aceitáveis para matar as células tumorais, mas que induzam apenas um mínimo de toxicidade para os 9 Vizentini-Silva, C. tecidos não cancerosos (JACKSON et al, 2011) o que vem de encontro aos sistemas nanométricos de liberação de fármacos.…”

Section: Lipossomas Como Sistemas De Liberação De Fármacosunclassified

Sistema lipossomal de ftalocianina de cloro-alumínio, contendo ácido fólico, aplicada à terapia fotodinâmica

Silva¹

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À minha amada e dedicada mãe. À todos que acreditam e torcem por mim.AGRADECIMENTOS À Deus pela minha vida e saúde. "Porque dEle, por Ele, e para Ele, são todas as coisas; glória, pois, a Ele eternamente." (Romanos 11:36) Ao meu orientador, Prof. Dr. Antonio Claudio Tedesco, pela oportunidade de aprendizado e crescimento, pela confiança e dedicação ao longo dos anos.À minha mãe, Francisca, por seu amor incondicional e sem limites; por toda atenção, afeto e suporte sem os quais eu jamais teria conseguido chegar até aqui.Ao meu pai, João Carlos, pelo amor e carinho, que mesmo longe fisicamente, estava sempre presente em meu coração.Às minhas irmãs, Flávia e Mirela, por me ensinarem a compartilhar, por caminharem ao meu lado nos momentos difíceis e por fazerem da minha família a melhor do mundo.Ao meu amor, amigo, companheiro e namorado, Weverton, por me ajudar a superar os desafios e tornar os meus dias mais felizes e à sua família pelo apoio e carinho.À toda a minha família, por serem meu alicerce e meu porto seguro. Pelos incentivos, mensagens de amor e fé e pelos almoços de domingo que me davam força para continuar.Aos amigos do Laboratório de Fotobiologia e Fotomedicina, mesmo os que não fazem mais parte do grupo, pelo dia-a-dia de trabalho e descontração e por partilharem seus conhecimentos. Em especial às amigas Dani, Gi e Paty Goto pela paciência, ensinamentos e companheirismo.Aos amigos de Ribeirão, por compartilharem comigo maus e ótimos momentos dos melhores anos da minha vida. Em especial às queridas e divertidas Bulma, Gorda, Daninha e Bruna.Aos amigos de Catanduva, pela amizade duradoura e sincera e por tornarem meus finais de semana alegres e inusitados. À Prof. Dra. Paula Rahal e à todos do Laboratório de Estudos Genômicos, em especial à Marília pela atenção e colaboração. À Universidade de São Paulo e aos docentes e funcionários da FFCLRP-USP.À CAPES pela concessão da bolsa de mestrado e pelo apoio financeiro para a realização desta pesquisa. . Palavras Terapia FotodinâmicaA Terapia Fotodinâmica (TFD) é um procedimento clinicamente aprovado desde 1993 no Canadá, 1996 no Japão e em 2001 na Europa (KAWASE e ISEKI, 2013;TRIESSCHEIJN et al., 2006), pois envolve uma terapêutica minimamente invasiva que pode induzir atividades citotóxicas seletivas para células cancerosas localizadas em regiões específicas (ALLISON e MOGHISSI, 2013;LIU et al., 2010). Na ultima década houve grande aumento de pesquisas envolvendo o uso da TFD para aplicação em diversas patologias não neoplásicas (SIQUEIRA-MOURA et al., 2013), principalmente após a aprovação do uso do medicamento A TFD é composta por três componentes essenciais: FF, luz e oxigênio molecular.Nenhum desses agentes é individualmente tóxico, mas juntos eles iniciam uma sequência de reações fotoquímicas e fotobiológicas que geram oxigênio singlete ( 1 O 2 ) e outras espécies reativas (AGOSTINIS et al., 2011;DOUGHERTY et al., 1998). Este é, portanto, um fator essencial na minimização de efeitos colaterais, já que, somente o FF que for ativado pela luz vi...

show abstract

Increased accumulation of paclitaxel and doxorubicin in proliferating capillary cells and prostate cancer cells following ultrasound exposure

Cited by 22 publications

References 35 publications

Low-frequency low energy ultrasound combined with microbubbles induces distinct apoptosis of A7r5 cells

Low-frequency low energy ultrasound combined with microbubbles induces distinct apoptosis of A7r5 cells

Treatment of PC-3 cells with ultrasound combined with microbubbles induces distinct alterations in the expression of Bcl-2 and Bax

Sistema lipossomal de ftalocianina de cloro-alumínio, contendo ácido fólico, aplicada à terapia fotodinâmica

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