Increased accumulation of pathogenesis-related proteins in response of lupine roots to various abiotic stresses

Response of antioxidant enzymes and some metabolic activities in wheat to Fusarium spp. Infections

“…The content of soluble, insoluble and total protein in the shoots showed an increase in wheat plants inoculated with all the Fusarium species (Fig. 5), as also recorded by other authors (Carporale et al, 2004;Przymusinski et al, 2004), who indicated that plants respond to pathogens by defence strategies leading to the synthesis of protective compounds such as pathogenesis-related proteins. Even though 100 different proteins from the PR-10 family have been identified, their biological function has not yet been clarified and most of the roles suggested are highly hypothetical.…”

Transcriptome Analysis of Drought Induced Stress in <i>Chenopodium quinoa</i>

“…Finally, a pathogenesisrelated gene protein (contig comp1469_c0_seq1) was overly expressed in Ollague in the severe drought-like treatment. Kitajima and Sato [57] reported that pathogennesis-related proteins are induced by hormones or ROS in response to biotic stress, while more recently Przymusinski et al [58] showed that an increase of these proteins can be induced as a response to various abiotic stresses.…”

Immunohisto- and cytochemical localization of PR-10 proteins induced by heavy metals in lupine roots

“…Lupine seedlings were incubated in a solution of Cu instead of Pb to avoid electron-dense lead deposits in cells exposed to this metal, which could hinder the analysis of gold particles localization with transmission electron microscopy. However, our earlier studies have indicated that Cu ions increase the synthesis and accumulation of PR-10 proteins in a manner analogous to lead treatment (Przymusiński et al 2004). In control root cells, the labeling was mainly distributed over the cytoplasm in the form of more or less evenly spaced gold particles (Fig.…”

“…The presence or accumulation of pr10 genes expression products, mostly under stress conditions, has been demonstrated in roots and leaves (Bantignies et al 2000;Przymusiński et al 2004;Utriainen et al 1998), hypocotyls (Gzyl et al 1997), tuber (Flores et al 2002), stems, seeds (Iturriaga et al 1994), fruits (Andersen et al 2011), embryos and pollen (Swoboda et al 1994), stigma (Constabel and Brisson 1995), calluses (Moiseyev et al 1997), and cell suspension (Carpin et al 1998). These data suggest that PR-10 proteins might be potentially accumulated in all organs of plants and are in line with our earlier study (Przymusiński et al 2001) in which the localization of PR (L1-L6) proteins with a tissue printing method was investigated in 12-day-old lupine seedlings, but excluding the root tips.…”

“…The vast majority of PR-10 class family proteins represent products of gene expression under bacterial or fungal pathogens stress conditions (Qiaojuan et al 2008;van Loon et al 1994;Walter et al 1996). However, previous studies carried out on lupine seedling root tips showed that heavy metal ions, such as lead, copper, cadmium, zinc (Przymusiński and Gwóźdź 1994;Przymusiński et al 2004) and chromium (unpublished data), strongly induce the synthesis and accumulation of 16 kDa polypeptides. Sequence analysis of 20 N-terminal amino acids indicated that all these polypeptides show a high degree of homology to the 10 class family of pathogenesisrelated proteins (Przymusiński and Gwóźdź 1999).…”