Increased acetylcholinesterase activities in specimens of Sparus auratus exposed to sublethal copper concentrations

Romani, Rita; Antognelli, Cinzia; Baldracchini, Francesca; Santis, Alessandra De; Isani, Gloria; Giovannini, Elvio; Rosi, Gabriella

doi:10.1016/s0009-2797(03)00058-9

Cited by 109 publications

(39 citation statements)

References 27 publications

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“…Besides, timely inductions of AChE activity were noted during the study period, especially in late summer where transplanted mussels exhibited cholinesterase activities 2.6-5.9 times higher than those recorded in native mussels. Similar inductions have been reported for zebra mussels from Lake Lugano (Italy) (Binelli et al 2005), probably as a result of Cu exposure as already noted in fish species (Dethloff et al 1999;Romani et al 2003). Enhanced AChE activity might be related to interactions of copper ions with AChE enzyme, substrate and coenzymes, resulting in protection of the enzyme against thermal inactivation, increased enzyme-substrate affinity or reduced energy activation (Jonsson and Aoyama 2010).…”

Section: Site Contaminationsupporting

confidence: 77%

One-year monitoring of core biomarker and digestive enzyme responses in transplanted zebra mussels (Dreissena polymorpha)

et al. 2012

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A 12-month active biomonitoring study was performed in 2008-2009 on the Vesle river basin (Champagne-Ardenne, France) using the freshwater mussel Dreissena polymorpha as a sentinel species; allochthonous mussels originating from a reference site (Commercy) were exposed at four sites (Bouy, Sept-Saulx, Fismes, Ardre) within the Vesle river basin. Selected core biomarkers (acetylcholinesterase (AChE) activity, glutathione-S transferase (GST) activity, metallothionein concentration), along with digestive enzyme activities (amylase, endocellulase) and energy reserve concentrations (glycogen, lipids), were monitored throughout the study in exposed mussels. At the Fismes and Ardre sites (downstream basin), metallic and organic contamination levels were low but still high enough to elicit AChE and GST activity induction in exposed mussels (chemical stress); besides, chemical pollutants had no apparent deleterious effects on mussel condition. At the Bouy and Sept-Saulx sites (upstream basin), mussels obviously suffered from adverse food conditions which seriously impaired individual physiological state and survival (nutritional stress); food scarcity had however no apparent effects on core biomarker responses. Digestive enzyme activities responded to both chemical and nutritional stresses, the increase in energy outputs (general adaptation syndrome-downstream sites) or the decrease in energy inputs (food scarcity-upstream sites) leading to mid- or long-term induction of digestive carbohydrase activities in exposed mussels (energy optimizing strategy). Complex regulation patterns of these activities require nevertheless the use of a multi-marker approach to allow data interpretation. Besides, their sensitivity to natural confounding environmental factors remains to be precised.

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Section: Site Contaminationsupporting

confidence: 77%

One-year monitoring of core biomarker and digestive enzyme responses in transplanted zebra mussels (Dreissena polymorpha)

et al. 2012

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“…*: P < 0.05, statistical significance compared with control. and in a fish species, Sparus aurata, after copper exposure and also in mussel after lead and cadmium exposures (Romani et al, 2003;Bainy et al, 2006;Güngördü et al, 2010). One of the possibilities for the increase of AChE activity by metals may be due to de novo synthesis of this enzyme as a response to an initial inhibition (Bainy et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Effect of nanosized TiO2 particles on the development of Xenopus laevis embryos

Birhanli¹,

Emre

Sayılkan³

et al. 2014

Turk J Biol

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This paper reports the toxic properties of nano-TiO 2 on 2 different early life stages of Xenopus laevis. Synthesis of nano-TiO 2 particles was carried out by the hydrothermal method. Characterization of nanoparticles was performed using spectral techniques including X-ray diffraction, scanning electron microscopy, and a particle-size analyzer. Embryos at the 8th stage and tadpoles at the 46th stage were exposed to 7 concentrations of nano-TiO 2 in the range of 5 to 320 ppm. After 96 h of exposure, the mortality percentage of each exposure concentration was calculated and the activity of enzyme biomarkers acetylcholinesterase, carboxylesterase, glutathione S-transferase, glutathione reductase, lactate dehydrogenase, and aspartate aminotransferase were determined in living embryos and tadpoles. None of the tested concentrations of TiO 2 caused statistically significant mortality or malformation (only for the embryo test) as compared to the control groups. Furthermore, we did not observe any significant changes in enzyme activities in tadpole samples from the 46th stage, although some minor changes not related to the concentrations were observed in embryos.

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“…2) can be attributed to several scenarios. Silver, either in nano or ionic form, could enhance Romani et al (2003) observed for sublethal Cu concentrations in fish. Another possibility for AChE activation by metals may be related to a de novo synthesis of the enzyme as a response to an initial inhibition.…”

Section: Ache Responsementioning

confidence: 98%

“…Literature data on the metal effects on AChE activities revealed some contradictory results. Metals have been described as both AChE inhibitors (Devi and Fingerman 1995;Suresh et al 1992;Beauvais et al 2001;Diamantino et al 2000) and stimulators (Jemec et al 2008;Romani et al 2003;Zatta et al 2002).…”

Section: Ache Responsementioning

confidence: 99%

Response of biochemical biomarkers in the aquatic crustacean Daphnia magna exposed to silver nanoparticles

Ulm

Krivohlavek

Jurašin

et al. 2015

Environ Sci Pollut Res

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The proliferation of silver nanoparticle (AgNP) production and use owing to their antimicrobial properties justifies the need to examine the resulting environmental impacts. The discharge of biocidal nanoparticles to water bodies may pose a threat to aquatic species. This study evaluated the effects of citrate-coated AgNPs on the standardized test organism Daphnia magna Straus clone MBP996 by means of biochemical biomarker response. AgNP toxicity was compared against the toxic effect of Ag(+). The toxicity endpoints were calculated based upon measured Ag concentrations in exposure media. For AgNPs, the NOAEC and LOAEC values at 48 h were 5 and 7 μg Ag/L, respectively, while these values were 0.5 and 1 μg Ag/L, respectively, for Ag(+). The EC50 at 48 h was computed to be 12.4 ± 0.6 and 2.6 ± 0.1 μg Ag/L for AgNPs and Ag(+), respectively, with 95 % confidence intervals of 12.1-12.8 and 2.3-2.8 μg Ag/L, respectively. These results indicate significant less toxicity of AgNP compared to free Ag(+) ions. Five biomarkers were evaluated in Daphnia magna neonates after acute exposure to Ag(+) or AgNPs, including glutathione (GSH) level, reactive oxygen species (ROS) content, and catalase (CAT), acetylcholinesterase (AChE), and superoxide dismutase (SOD) activity. AgNPs induced toxicity and oxidative stress responses in D. magna neonates at tenfold higher concentrations than Ag. Biochemical methods revealed a clear increase in AChE activity, decreased ROS level, increased GSH level and CAT activity, but no significant changes in SOD activity. As Ag(+) may dissolve from AgNPs, these two types of Ag could act synergistically and produce a greater toxic response. The observed remarkably high toxicity of AgNPs (in the parts-per-billion range) to crustaceans indicates that these organisms are a vulnerable link in the aquatic food chain with regard to contamination by nanosilver. Graphical Abstract ᅟ.

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Increased acetylcholinesterase activities in specimens of Sparus auratus exposed to sublethal copper concentrations

Cited by 109 publications

References 27 publications

One-year monitoring of core biomarker and digestive enzyme responses in transplanted zebra mussels (Dreissena polymorpha)

One-year monitoring of core biomarker and digestive enzyme responses in transplanted zebra mussels (Dreissena polymorpha)

Effect of nanosized TiO2 particles on the development of Xenopus laevis embryos

Response of biochemical biomarkers in the aquatic crustacean Daphnia magna exposed to silver nanoparticles

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