Increased adherence to human epithelial cells of resistant Pseudomonas aeruginosa strains

Outer surface changes of Pseudomonas aeruginosa in relation to resistance to gentamicin and carbenicillin

“…Alterations of OM protein have also been reported for P. aeruginosa mutants resistant to polymyxin, EDTA and gentamicin (Nicas and Hancock, 1980). It has also been shown that variants of P. aeruginosa habituated to increased concentrations of carbenicillin or gentamicin are characterised by increased adherence, evidently due to an altered surface as detected by electronmicroscopy (Lianou et al, 1980). The morphological responses of P. aeruginosa to N-formimidoyl thienamycin, azthreonam, ceftazidime and cefoperazone were studied by transmission electronmicroscopy (Elliot and Greenwood, 1984).…”

Phenotypic factors correlated with the absence of virulence among gentamicin-resistant Pseudomonas aeruginosa strains

“…The clinically derived GRPA strains, when compared with GSPA isolates, demonstrated increased adherence to epithelial cells and reduced lipolytic activity and chemotactic motility. However, unlike in vitro-derived GRPA isolates, the clinical GRPA strains failed to demonstrate auxotrophy or alterations in glucose oxidation or increased sensitivity to membrane-active differential dyes (3,12,20). Previous studies with the burned rat model have demonstrated that chemotactic motility along with proteolytic activity of P. aeriuginosa are important virulence factors in the post-colonization events of burn tissues leading to subsequent invasion with bacteremia and death (2, 7,14,16).…”

“…Experiments with laboratory animals (rats) support this conclusion since GRPA isolates have been shown to be less virulent than GSPA strains (11). In addition, a number of phenotypic properties have been ascribed to laboratory-generated GRPA isolates, including auxotrophy, lack of agglutination in monospecific antisera, changes in phage attachment sites, and increased adherence to human epithelial cells when compared with GSPA progenitor strains (3,12). All of these data suggest fundamental alterations to the outer membrane-cell wall of GRPA isolates.…”

Προσκολλητικότητα Και Λειτουργίες Των Πολυμορφοπύρηνων Λευκών Αιμοσφαιρίων Σε Χρόνιες Ηπατοπάθειες

“…(100) . Επίσης στελέχη ψευδομονάδας ανθεκτικά στη γενταμυκίνη εμφανίζουν μεγαλύτερη προσκολλητικότητα στα επιθηλιακά κύτταρα διαφόρων συστημάτων συγκριτικά με τα ευαίσθητα στελέχη (101) . Τα ανθεκτικά όμως στελέχη παράγουν χημειοτακτικό παράγοντα και είναι ευαίσθητα στη φαγοκυττάρωση και βακτηριοκτόνο δράση των ΠΜΠ (102) (109) , σακχαρώδη διαβήτη, βαριές αναιμίες <111) , και όσοι ελάμβαναν αντιβιοτικά (110 • 116) ή ανοσοκατασταλτικά φάρμακα (117) .…”