1992
DOI: 10.1007/bf01245357
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Increased binding of3H-L-deprenyI in spinal cords from patients with amyotrophic lateral sclerosis as demonstrated by autoradiography

Abstract: The present investigation has applied quantitative autoradiography and histochemistry to study the regional distribution of MAO-B and its relation to the number of cells in respective regions. L-deprenyl binds irreversibly and quantitatively to the B-form of monoamine oxidase, MAO, and is an ideal 3H-ligand to measure the MAO-B enzyme protein in tissues by means of in vitro autoradiography. The investigation is performed on spinal sections from five controls and five cases with amyotrophic lateral sclerosis (A… Show more

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Cited by 33 publications
(14 citation statements)
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“…The increased [ 11 C]-DED binding rate in white matter in the patients accords well with previous neuropathological findings of widespread astrocytosis in the subcortical white matter in ALS [2]. Astrocytosis in ALS has also been demonstrated post mortem in other parts of the corticospinal tract but pons has not been included [3,13]. Since we lack neuropathological support in this region, we must interpret the increased binding rate in pons cautiously.…”
Section: Discussionsupporting
confidence: 84%
See 1 more Smart Citation
“…The increased [ 11 C]-DED binding rate in white matter in the patients accords well with previous neuropathological findings of widespread astrocytosis in the subcortical white matter in ALS [2]. Astrocytosis in ALS has also been demonstrated post mortem in other parts of the corticospinal tract but pons has not been included [3,13]. Since we lack neuropathological support in this region, we must interpret the increased binding rate in pons cautiously.…”
Section: Discussionsupporting
confidence: 84%
“…Using this technique we have demonstrated increased [ 3 H]-L-deprenyl binding in regions of neurodegeneration in ALS, e.g. motor neuron laminae and corticospinal tracts [13,14]. We have also found a high correlation between [ 3 H]-L-deprenyl binding and glial cell count supporting astrocytic localization of MAO-B within these areas.…”
Section: Introductionmentioning
confidence: 81%
“…11,12) Both forms appear to be important for neurotransmitter regulation, and fluctuations in functional MAO activity may be associated with human diseases such as Parkinson's disease, depression and certain psychiatric disorders. [13][14][15][16][17] In the human brain, MAO-B predominates (MAO-B : MAO-Aϭ4 : 1) and is associated mainly with glial cells. 18) Unlike most enzyme or neurotransmitter receptors and transporters, MAO-B activity increases with normal aging and in neurodegenerative disease in glial cells in response to age-related or disease-associated neuron loss and gliosis.…”
mentioning
confidence: 99%
“…As neurotrophic factors are also produced by and released from glial cells, the increased levels of neurotrophic factors observed locally in the CSF could be a secondary event due to gliosis, which has been shown to occur both in ALS brain [55] and spinal cord [56]. Both astrocyte [57] and microglial cell [58] counts are increased in ALS spinal cord. Activated astrocytes [59][60][61] and microglia [62] have the capacity to produce and release increased amounts of trophic agents into the surrounding tissue.…”
Section: Neurotrophic Factor Production Release and Transportmentioning
confidence: 99%