Increased biomass and pigment production from Cassia alata L. callus cultures and their potential as a textile dye

Shah, M. Rauf; George, Indu Anna

doi:10.1016/j.indcrop.2018.11.018

Cited by 12 publications

(5 citation statements)

References 13 publications

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“…It showed that the concentration of 2,4-D 0.1 ppm with the BAP concentration of 2 ppm was the optimal concentration in the formation explant callus of potato sprout stem (Figure 6). As stated by Shah and George, (2019), the combination of auxin and cytokinin produced a better callus induction and biomass than adding only one growth regulator. Cytokinins are hormones involved in cell division.…”

Section: Resultsmentioning

confidence: 89%

Callus Development from Potato (Solanum tuberosum) Stem at Various Concentrations of Benzylaminopurine

Aprilia

Setiari²,

Nurchayati³

2022

J Bio Bio Edu

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Potato has the potential for food diversification.The propagation method in a short period is needed. One of the methods used is plant tissue culture. Benzyl Amino Purine (BAP), determine the success of propagation by tissue culture method. This research aimed to study the use of different BAP concentrations for callus development from stem explants. The explants put on culture medium added by 0, 1, 2, 3 ppm BAP. The development of the stem explant observed every week for a month. The results showed that callus formed in all media. Without BAP treatment, callus were formed after 2 weeks and got browning, then stopped growing. Callus grew and showed differentiation by application of all the BAP concentration. Callus greowth was optimally at 2 ppm BAP treatment. The callus from 1 ppm BAP produced the most number of roots, shoots and leaves than another concentration. This experiment showed that different BAP concentrations affected callus development of S. tuberosum from stem explant. The conclusion was callus growth has obtained by the treatment of 2 ppm BAP, while the development of callus has obtained on addition of 1 ppm BAP. The novelty of this research is the callus induction method from potato sprout stems grown from potato seeds with plant growth regulators Benzyl Amino Purine. Callus induction method from potato sprout stems grown from potato seeds can be a guide for embryogenic callus induction.

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Section: Resultsmentioning

confidence: 89%

Callus Development from Potato (Solanum tuberosum) Stem at Various Concentrations of Benzylaminopurine

Aprilia

Setiari²,

Nurchayati³

2022

J Bio Bio Edu

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“…These dyes are categorized as either fat-soluble or water-soluble. The dye extracted from C. alata flowers contains anthraquinones, natural pigments that produce yellow shades, and excellent light fastness and wash fastness properties [19] , [20] , [21] , [22] . Anthraquinones have biomedical applications, such as natural antimicrobial agents, and the extraction parameters influence their quantity and stability.…”

Section: Resultsmentioning

confidence: 99%

An eco-friendly ultrasound approach to extracting yellow dye from Cassia alata flower petals: Characterization, dyeing, and antibacterial properties

Muruganandham

Sivasubramanian

Velmurugan

et al. 2023

Ultrasonics Sonochemistry

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“…It showed that the concentration of 1 ppm NAA with 1 ppm of BAP was the optimal concentration in the formation callus of tomato sprout. As stated by Shah & George (2019), the combination of auxin and cytokinin produces a better callus induction and biomass than adding only one growth regulator. The highest fresh weight was found in the subculture treatment three times (9.21 g) and the lowest weight was found in the treatment without subculture with a mean weight of 3.22 g ( Table 1).…”

Section: The Fresh Weight Of Callusmentioning

confidence: 97%

Effect of Subculture Frequency Toward Growth And Carotenoid Content from Tomato (Lycopersicon Esculentum Mill.) Callus

et al. 2020

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Tomatoes (Lycopersicon esculentum Mill.) are a source of carotenoids they are easy to find. These compounds function as precursors of vitamin A, antioxidant, and prevent cancer. The extraction of carotenoid compounds for commercial products usually uses fresh plants, which are less efficient and require a lot of raw materials. The supply of these raw materials can be done through tissue culture. The frequency of subculture or supply of nutrients in tissue culture is very influential on the content of callus carotenoids produced. This study aimed to determine the effect of subculture frequency on growth, development and callus carotenoid content and to find out the right frequency of subculture to produce callus with optimal growth, development and carotenoid content. The design this study was a single Completely Randomized Design (CRD) with 4 treatments of subculture frequency and 5 replications. The data obtained were analyzed by ANOVA at the 95% test level followed by DMRT in case a significant different was found The results showed subculture frequency affected growth, development and carotenoid content of callus Lycopersicon esculentum. Mill. The most optimal treatment to induce growth and production of carotenoids in this study was treatment of thrice subculture while the most optimal treatment in inducing development was the twice subculture treatment.

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Increased biomass and pigment production from Cassia alata L. callus cultures and their potential as a textile dye

Cited by 12 publications

References 13 publications

Callus Development from Potato (Solanum tuberosum) Stem at Various Concentrations of Benzylaminopurine

Callus Development from Potato (Solanum tuberosum) Stem at Various Concentrations of Benzylaminopurine

An eco-friendly ultrasound approach to extracting yellow dye from Cassia alata flower petals: Characterization, dyeing, and antibacterial properties

Effect of Subculture Frequency Toward Growth And Carotenoid Content from Tomato (Lycopersicon Esculentum Mill.) Callus

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