Increased calcitonin gene-related peptide- and cholecystokinin-like immunoreactivities in spinal motoneurones after dorsal rhizotomy

Taquet, H.; Plachot, J. J.; Pohl, Michel; Collin, E.; Benoliel, J.J.; Bourgoin, S.; Mauborgne, A.; Meunier, Jean‐Claude; François, Clément; Hamon, Michel

doi:10.1007/bf01244818

Cited by 11 publications

(4 citation statements)

References 45 publications

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“…The present results indicate that unilateral lesioning of the dorsal root sensory input alone has only limited effect on peptide expression in the segmental motoneuron and then only in the acute postlesion survival period. This is in line with a study by Hirakawa and Kawata (1992), but somewhat in contrast to the finding by Taquet et al (1992), who reported a significant increase in the levels of CGW-LI in motoneurons 10 days after dorsal rhizotomy. In our study, CGRP-LI staining in the ventral horn was only increased 1 day postoperatively and this was seen bilaterally, while mRNA expression was up-regulated only ipsilaterally.…”

Section: Role Of Afference To the Rnotoneuronsupporting

confidence: 92%

GAP‐43, aFGF, CCK and α‐ and β‐CGRP in Rat Spinal Motoneurons Subjected to Axotomy and/or Dorsal Root Severance

Piehl

Arvidsson

Johnson

et al. 1993

Eur J of Neuroscience

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The mRNA levels for growth-associated protein 43 (GAP-43), acidic fibroblast growth factor (aFGF), alpha- and beta-calcitonin gene-related peptide (CGRP), cholecystokinin (CCK) and choline acetyltransferase (ChAT) in rat lumbar spinal motoneurons were studied by in situ hybridization 1, 5 and 21 days and 20 weeks following unilateral peripheral nerve sectioning, ventral rhizotomy or dorsal rhizotomy. Furthermore, CGRP- and aFGF-like immunoreactivities in the ventral horn were studied using immunohistochemistry. One to 21 days after axotomy, GAP-43 and alpha-CGRP mRNAs increased in lesioned motoneurons, while the aFGF mRNA levels were marginally higher in motoneurons on the lesion side as compared to the control side. beta-CGRP, CCK and ChAT mRNA levels, on the other hand, decreased during the short-term response (1-21 days) to axotomy. After ventral rhizotomy, but not peripheral axotomy, there was complete disappearance of aFGF-like immunoreactivity in the ventral root proximal to the lesion. In animals subjected to long-term survival (20 weeks) after peripheral axotomy, the expression of all studied substances had returned to normal levels. Unilateral dorsal rhizotomy did not induce any substantial short- or long-term shifts in the cellular expression of the GAP-43, aFGF, CGRP and CCK peptides or their mRNAs in motoneurons of lesioned segments. These results indicate that peptides/proteins in motoneurons are expressed differentially after axotomy. Whereas alpha-CGRP and GAP-43 are up-regulated, CCK and beta-CGRP become down-regulated and aFGF is largely unaffected.

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Section: Role Of Afference To the Rnotoneuronsupporting

confidence: 92%

GAP‐43, aFGF, CCK and α‐ and β‐CGRP in Rat Spinal Motoneurons Subjected to Axotomy and/or Dorsal Root Severance

Piehl

Arvidsson

Johnson

et al. 1993

Eur J of Neuroscience

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“…For example, increased CGRP-IR in motoneurons located in the ventral horn, the trochlear nucleus, the facial nucleus, and the hypoglossal nucleus have been reported following the transection of the sciatic (Arvidsson et al, 1990), trochlear (Wang et al, 1993), facial (Streit et al, 1989), and hypoglossal (Grothe, 1993) nerves, respectively. Increased CGRP-IR in ventral horn motoneu-Pons has also been reported following both ventral and dorsal root section (Hirakawa and Kawata, 1992) and dorsal root section alone (Taquet et al, 1992).…”

Section: Discussionmentioning

confidence: 74%

Light microscopic localization of calcitonin gene-related peptide in the normal feline trigeminal system and following retrogasserian rhizotomy

et al. 1996

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Calcitonin gene-related peptide (CGRP) is a neuropeptide that has been implicated in the transmission and modulation of primary afferent nociceptive stimuli. In this study, we describe the light microscopic distribution of CGRP immunoreactivity (IR) within the feline trigeminal ganglion and trigeminal nucleus of normal adult subjects and in subjects 10 and 30 days following complete retrogasserian rhizotomy. Within the trigeminal ganglion of normal subjects, cell bodies and fibers showed CGRP-IR, whereas immunoreactive fibers were rare in the central root region. Within the normal spinal trigeminal and main sensory nuclei, CGRP-IR was seen to form a reproducible pattern that varied between the different nuclei. Following rhizotomy, most, but not all, of the CGRP-IR was lost from the spinal trigeminal and main sensory nuclei, except in regions where the upper cervical roots and cranial nerves VII, IX and X project into the trigeminal nucleus. The pattern seen at 10 days contained more CGRP-IR than that seen at 30 days and suggests that degenerating fibers still show CGRP-IR. In contrast to the decrease seen in the nuclei after rhizotomy, examination of the central root that was still attached to the trigeminal ganglion showed an increase in CGRP-IR within fibers, some of which ended in growth conelike enlargements. Rhizotomy induced a dramatic increase in CGRP-IR within trigeminal motoneurons and their fibers, which was strongest 10 days after rhizotomy and weaker at 30 days, which was still stronger than normal. These results indicate that the majority of CGRP-IR found in the trigeminal nucleus originates from trigeminal primary afferents and that an upregulation of CGRP-IR occurs in trigeminal motoneurons and in regenerating fibers in the part of the central root that was still attached to the ganglion. In addition, the persistence of CGRP-IR fibers in the trigeminal nucleus provides one possible explanation for the preservation of pain in humans following trigeminal rhizotomy.

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“…It has never previously been examined whether or not neuropeptide expression in motoneurons changes in response to irradiation, whereas these neurons have been extensively studied with respect to neuropeptide expression after other experimental situations. Thus, substantial changes in the levels of CGRP in spinal cord motoneurons are observed in response to axotomy (Arvidsson et al, 1990;Caldero et al, 1992), dorsal rhizotomy (Taquet et al, 1992), and spinal cord transection (Arvidsson et al, 1989;Marlier et al, 1990). Furthermore, the expression of several peptides is dramatically altered in spinal cord motoneurons after axotomy (Zhang et al, 1993).…”

Section: Discussionmentioning

confidence: 99%

Effects of irradiation on neuropeptide expression in rat salivary gland and spinal cord

et al. 1994

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It is well-known that a large number of factors can influence the expression of neuropeptides in the nervous system. In the present study, the effects of unilateral and bilateral irradiation to the rat head and neck on the expression of neuropeptides in the innervation of the submandibular gland and in the ganglionic cells of the submandibular ganglion was examined ten days and six months after treatment. Antisera directed against enkephalin and bombesin and immunohistochemical methods were used. The effects of bilateral irradiation on the staining pattern of various neuropeptides in the cervical spinal cord were also studied. In the submandibular gland and in the submandibular ganglionic cells, there was a markedly increased neuropeptide expression ten days after bilateral treatment, as seen after staining with both antisera used, while no changes occurred after unilateral treatment. Six months after treatment, the pattern of neuropeptide expression in the submandibular gland/ganglion corresponded to that seen in controls. Irradiation did not lead to any changes in the staining pattern of neuropeptides in the spinal cord. The observations show that there is a great complexity in the susceptibility of nervous tissues to radiotherapy with respect to influences on the expression of neuropeptides.

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Increased calcitonin gene-related peptide- and cholecystokinin-like immunoreactivities in spinal motoneurones after dorsal rhizotomy

Cited by 11 publications

References 45 publications

GAP‐43, aFGF, CCK and α‐ and β‐CGRP in Rat Spinal Motoneurons Subjected to Axotomy and/or Dorsal Root Severance

GAP‐43, aFGF, CCK and α‐ and β‐CGRP in Rat Spinal Motoneurons Subjected to Axotomy and/or Dorsal Root Severance

Light microscopic localization of calcitonin gene-related peptide in the normal feline trigeminal system and following retrogasserian rhizotomy

Effects of irradiation on neuropeptide expression in rat salivary gland and spinal cord

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