Despite the need to analyze sex hormones and prostanoid lipids together, few previous studies have focused on investigating the combination of the two different groups simultaneously in a single run due to their closely-related structures; most methods are limited to analyzing either one or the other [11-13]. Studying either the prostanoid or sex hormone group in isolation neglects the interaction between PGs and sex hormones in the progression of disease, and thus brings only partial evidence to the interpretation of its molecular mechanism. We have developed a method to quickly separate and quantify