Increased collection efficiency of CD34+ cells after mobilization with preemptive use of plerixafor followed by leukocytapheresis on the same day

Abstract

BACKGROUND Plerixafor should be administered 6 to 11 hours before starting leukocytapheresis. However, we have been using plerixafor followed by leukocytapheresis according to different time schedules since 2007. Our objective was to compare the CD34+ cell collection efficiency (CE1) of the first leukocytapheresis performed after using plerixafor at different time intervals. STUDY DESIGN AND METHODS Same‐day schedule refers to the administration of plerixafor at 10:00 AM and starting the leukocytapheresis on the same day at 4:00 PM (6 hours interval). Next‐day schedule refers to the administration of plerixafor at 8:00 PM and starting the leukocytapheresis on the next day (10:00 AM or 4:00 PM; either a 14‐ or 20‐hr interval). Variables that might influence the CE1 of CD34+ cells were analyzed by longitudinal linear regression with a random effects model derived by generalized estimating equations. RESULTS The median CE1 of CD34+ cells was higher in the group of 30 patients who underwent leukocytapheresis on the same day when compared with the group of 62 patients who underwent leukocytapheresis on the next day (65.8% vs. 56.7%; p < 0.01). In the longitudinal linear regression analysis, only the time from plerixafor administration to leukocytapheresis start was associated with a statistically significant decrease in the CE1 of CD34+ cells (CE1 change −0.034%; p < 0.01). CONCLUSION Higher CE1 of CD34+ cells was observed when patients underwent leukocytapheresis on the same day after receiving plerixafor in comparison with administering plerixafor and underwent leukocytapheresis on the next day. Larger studies are necessary to confirm present results.

Use of plerixafor to mobilize haematopoietic progenitor cells in healthy donors

“…Thus, a CD34+ cell count can be performed in the morning, administering plerixafor if the count is poor and performing an apheresis session in the afternoon of the same day. Our group found that in autologous donors, CD34+ cell collection efficiency of same-day processes was higher when compared with next-day apheresis [58]. A Dutch group has shown a good correlation between data obtained with a fully automated haematology analyser and CD34+ cell count by flow cytometry.…”

Use of Plerixafor for Stem Cell Mobilization in the Setting of Autologous and Allogeneic Stem Cell Transplantations: An Update

“…One recent study has shown that the efficacy is higher if apheresis was performed on the same day as the administration of plerixafor. 40 However, there are limited studies regarding the effect of plerixafor on the kinetics of stem cell mobilization.…”

Evaluation of CD34+ Cell Count at Different Time Points Following Plerixafor Administration in Autologous Hematopoietic Stem Cell Transplantation

“…reported an approximately seven-fold increase in circulating CD34+ cells after 6 hours of plerixafor injection (22). In a study conducted in 2020, a comparison between apheresis products of two groups with 6-hour intervals and 14 to 20-hours intervals indicated higher stem cell dose in the first group (23). A study on sickle cell disease patients illustrated a peak above 80 CD34+/µL in two to three hours after drug administration (24).…”