Increased concentration of T‐cell receptor rearrangement excision circles (<scp>TREC</scp>) in peripheral blood in Graves' disease

Strawa, Katarzyna; Markowska, Anna; Miśkiewicz, Piotr; Kuś, Aleksander; Ambroziak, Urszula; Szymański, Konrad M.; Zbiec, Renata; Spólnicka, Magdalena; Krajewski, Paweł; Bednarczuk, Tomasz; Płoski, Rafał

doi:10.1111/cen.12492

Cited by 4 publications

(8 citation statements)

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“…Examination of the decrease in the number of sjTREC molecules caused by thymus involution occurring in the course of human life has a predictive capacity and has been implemented in forensic age prediction [ 33 ]. Our investigation of age predictive DNA methylation markers does not support a conclusion that can be drawn from the study performed by Strawa about younger age of GD patients [ 32 ]. A hypomethylation of the FHL2 gene predicts younger age of patients, but two other markers (TRIM59 and KLF14) have increased methylation comparing to the controls, while ELOVL2 and C1orf132 are unchanged.…”

Section: Discussioncontrasting

confidence: 93%

“…Antibody-driven activation of the thyrotropin receptor leads to hyperfunction of the thyroid gland and thyroid enlargement and in consequence increased production of the thyroid hormone [ 31 ]. Previous study investigated T cell receptor rearrangement excision circle (sjTREC) concentration in Graves’ disease patients and reported that although generally decreasing with age, it was significantly higher in GD group compared with controls [ 32 ]. Examination of the decrease in the number of sjTREC molecules caused by thymus involution occurring in the course of human life has a predictive capacity and has been implemented in forensic age prediction [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

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DNA methylation in ELOVL2 and C1orf132 correctly predicted chronological age of individuals from three disease groups

et al. 2017

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Improving accuracy of the available predictive DNA methods is important for their wider use in routine forensic work. Information on age in the process of identification of an unknown individual may provide important hints that can speed up the process of investigation. DNA methylation markers have been demonstrated to provide accurate age estimation in forensics, but there is growing evidence that DNA methylation can be modified by various factors including diseases. We analyzed DNA methylation profile in five markers from five different genes (ELOVL2, C1orf132, KLF14, FHL2, and TRIM59) used for forensic age prediction in three groups of individuals with diagnosed medical conditions. The obtained results showed that the selected age-related CpG sites have unchanged age prediction capacity in the group of late onset Alzheimer’s disease patients. Aberrant hypermethylation and decreased prediction accuracy were found for TRIM59 and KLF14 markers in the group of early onset Alzheimer’s disease suggesting accelerated aging of patients. In the Graves’ disease patients, altered DNA methylation profile and modified age prediction accuracy were noted for TRIM59 and FHL2 with aberrant hypermethylation observed for the former and aberrant hypomethylation for the latter. Our work emphasizes high utility of the ELOVL2 and C1orf132 markers for prediction of chronological age in forensics by showing unchanged prediction accuracy in individuals affected by three diseases. The study also demonstrates that artificial neural networks could be a convenient alternative for the forensic predictive DNA analyses.

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Section: Discussioncontrasting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

DNA methylation in ELOVL2 and C1orf132 correctly predicted chronological age of individuals from three disease groups

et al. 2017

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“…Impaired central tolerance in autoimmune endocrine disorders is a relatively new concept. However, up to this date, only a few studies have investigated circulating TRECs in patients with T1D [15], Hashimoto thyroiditis [36], and Graves disease [16, 37], and there are no data regarding KREC measurement in any autoimmune endocrine disorders. Hofer et al [15] observed increased circulating TRECs in 38 patients with T1D with mean age of 10 to 15 years.…”

Section: Discussionmentioning

confidence: 99%

“…The primer sequences for TREC assay were designed by Primer-3 software (National Center for Biotechnology Information, Bethesda, MD), whereas the probe was described earlier by Douek et al [28]. Primers and probe for KRECs were described earlier by Sottini et al [29] and those for albumin gene by Strawa et al [16]. The sequences of the primers, fluorescent probes, and amplicon sizes are given in Table 1.…”

Section: Methodsmentioning

confidence: 99%

“…The six dilution points were put in triplicate in the q-PCR assay to generate the standard curve. As previously reported [16], three standard curves were put, during the whole study, at the beginning, in the middle, and at the end of the q-PCR assay and are used to generate a composite standard curve and a regression equation for absolute and relative quantification. The interassay and intra-assay coefficients of variation for TRECs and KRECs for absolute copies were 26.1% and 21.0% and 6.2% and 12.6%, respectively.…”

Section: Methodsmentioning

confidence: 99%

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Central Immune Tolerance of T and B Cells in Patients With Idiopathic Hypoparathyroidism, T1D, and Autoimmune Thyroiditis

Mahtab

Kar

Saha

et al. 2019

Journal of the Endocrine Society

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Context Pathogenesis of idiopathic hypoparathyroidism (IH) is under investigation. Abnormalities in central immune tolerance have yet not been investigated in this condition. T-cell receptor excision circles (TRECs) and kappa-deleting recombination excision circles (KRECs), formed during receptor gene rearrangements, are tools to assess central T- and B-cell output. Objective We assessed the number of circulating TRECs and KRECs in patients with IH, autoimmune type 1 diabetes (T1D), and autoimmune thyroiditis (ATs) and healthy controls (HCs). Design Comparative case-control at tertiary care center. Subjects and Methods Absolute and relative TRECs and KRECs were measured in DNA extracted from whole blood of patients with IH (n = 181, 22 of whom were reassessed after a decade of follow-up) and T1D (n = 133), AT (n = 53), and HC (n = 135) using a quantitative real-time PCR/TaqMan ® probe technique. Results Absolute and relative means of TRECs and KRECs in IH were comparable to HCs, and no differences were found between IH with and without calcium-sensing receptor antibodies or class I HLA-A*26:01 association. TRECs and KRECs did not change after a decade of follow-up. T1D had significantly higher absolute TRECs than IH, AT, and HCs, whereas AT patients showed lower TRECs and the highest KRECs; these levels showed no noteworthy correlation with thyroid dysfunctions. Conclusion Patients with IH showed TRECs and KRECs comparable to HCs, indicating an intact mechanism of T- and B-cell central immune tolerance. Interestingly, absolute TRECs were significantly higher in T1D than HCs, suggesting impaired central immune tolerance in T1D.

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Juvenile myasthenia gravis in Norway: A nationwide epidemiological study

Popperud

Boldingh

Brunborg

et al. 2017

European Journal of Paediatric Neurology

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Increased concentration of T‐cell receptor rearrangement excision circles (TREC) in peripheral blood in Graves' disease

Abstract: Patients with GD, particularly those with hyperthyroidism, have increased concentration of TREC which may suggest increased rather than decreased thymic activity. Thus, GD does not follow the paradigm suggested for other autoimmune disorders which links autoimmunity with thymic senescence.

Cited by 4 publications

References 33 publications

DNA methylation in ELOVL2 and C1orf132 correctly predicted chronological age of individuals from three disease groups

DNA methylation in ELOVL2 and C1orf132 correctly predicted chronological age of individuals from three disease groups

Central Immune Tolerance of T and B Cells in Patients With Idiopathic Hypoparathyroidism, T1D, and Autoimmune Thyroiditis

Juvenile myasthenia gravis in Norway: A nationwide epidemiological study

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