Increased copper levels inhibit denitrification in urban soils

Li, Shun; Yang, Xiao-Ru; Buchner, Daniel; Wang, Haitao; Xu, Huijuan; Haderlein, Stefan B.; Zhu, Yan

doi:10.1017/s1755691018000592

Cited by 7 publications

(2 citation statements)

References 43 publications

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“…As a key component of Earth’s critical zone, the urban ecosystem has received growing concerns for studying N biogeochemistry. To date, studies documenting urban microbial N cycling predominantly focus on soils − and waters. , In contrast, the plant phyllosphere is an understudied microbial habitat for evaluating the interactions between residing N-metabolizing microbes and urban environment changes.…”

Section: Introductionmentioning

confidence: 99%

Hydrological Perturbations Facilitated Phyllosphere Denitrification of an Urban Greening Tree

Yi-fang

Huang

et al. 2022

ACS Earth Space Chem.

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In urban ecosystems, nutrient stocks, such as carbon (C), nitrogen (N), and phosphorus (P), are anthropogenically being enriched for material and energy supply. The overloaded nutrients have adverse ecological consequences, such as causing eutrophication of waters and soil in urban areas. Studying the ecophysiology of nutrient-cycling microbes in urban areas is the foundation to explore strategies for removing such excessive nutrients. The phyllosphere is an understudied microbial habitat for examining how the urban microbiome responds to common environmental changes, such as hydrological perturbations. Here, we investigated how successive rainy–sunny cycles within a season affect the genetic potential (gene abundances) for leaf nutrient cycling and particularly the functional potential (enzyme activities) for leaf denitrification of the greening tree Photinia fraseri. Of 41 detected C, N, P, and sulfur (S) cycling genes using high-throughput quantitative polymerase chain reaction, rainfalls only significantly (p < 0.05) increased the abundances of denitrification marker genes nirK and nirS and one C-fixation gene on the phyllosphere while having no significant impacts on other nutrient-cycling genes. The nirK and nirS genes encode nitrite reductases, which catalyze the hallmark step of the denitrification process. Further, a denitrification enzyme activity assay of phyllosphere microbiota showed that, in comparison to sunny weather, rainfalls significantly promoted nitrate reduction (5.48 μmol of NO3 – g–1 h–1; p < 0.001) and N2O production (2.07 nmol of N2O g–1 h–1; p < 0.05) rates, respectively. Together, this study revealed that hydrological perturbations can affect tree phyllosphere denitrification. Understating the ecophysiology of urban phyllosphere denitrifying microbes might be important for developing suitable phylloremediation strategies to attenuate urban N inputs.

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Section: Introductionmentioning

confidence: 99%

Hydrological Perturbations Facilitated Phyllosphere Denitrification of an Urban Greening Tree

Yi-fang

Huang

et al. 2022

ACS Earth Space Chem.

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“…Cu accumulation in urban soils, resulting from urbanization, may generally influence denitrification in urban ecosystems [13]. Copper ions significantly reduce the quality of ornamental plants.…”

Section: Introductionmentioning

confidence: 99%

Increasing the tolerance to copper of ornamental plants and lawn grasses in urban ecosystems

Gladkov¹,

Tashlieva²,

Dolgikh³

et al. 2021

Bull. Soc. Roy. Sc. de Liège

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The plants are an important part of the urban ecosystem. Plants help to reduce the level of pollution and the effect of adverse environmental factors on urban ecosystems. Among the most dangerous pollutants for plants are copper ions. Copper is one of the main pollutants of urban soil. Copper ions significantly reduce the quality of ornamental plants. One approach to solve this problem is to create plants resistant to adverse environmental factors using cell selection. This method is hardly ever used in urban greening. The aim of the work is to produce plants resistant to copper ions. It was developed technologies to obtain ornamental plants resistant to copper. These plants have substantially increased their resistance to copper. Plants Festuca rubra were resistant to high levels of copper contamination and can grow on the basis of the level of pollution along roads, in residential areas. Plants Brachycome iberidifolia proved to be resistant to the average level of pollution of copper and can grow in parks and squares.

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Ensifer meliloti denitrification is involved in infection effectiveness and N2O emissions from alfalfa root nodules

et al. 2023

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Purpose Alfalfa is one of the most valuable forage crops in temperate climate zones. Ensifer meliloti, the endosymbiont of alfalfa, contains all the denitrification genes but the capacity of alfalfa root nodules to produce nitrous oxide (N2O) is not known. In this work, N2O emissions as well as the influence of bacteroidal denitrification on nodulation competitiveness and N2O release from alfalfa nodules has been investigated. Methods Medicago sativa cv. Victoria plants were inoculated with E. meliloti 1021, a periplasmic nitrate reductase (Nap) defective mutant, a Nap overexpressing strain and a nitrous oxide reductase defective mutant. Plants were grown in the presence of different nitrate and copper treatments and subjected to flooding during one week before harvesting. N2O production by the nodules was analysed by using gas chromatography. Methyl viologen-dependent nitrate reductase (MV+-NR), nitrite reductase (MV+-NIR) and nitrous oxide reductase (N2OR) enzymatic activities were measured in isolated bacteroids. Results Alfalfa root nodules produce N2O in response to nitrate and flooding. Overexpression of Nap improved nodulation competitiveness and induced N2O emissions from nodules. Copper is required for an effective symbiosis as well as triggered a reduction of N2O production due to the induction of the N2OR and a reduction of NIR activities in the bacteroids. Conclusion Alfalfa root nodules emit N2O. Nap is involved in nodulation competitiveness and in N2O emissions by the nodules. Bacteroidal N2OR and NIR activities are modulated by Cu and may be considered as effective targets for the mitigation strategies of N2O emissions derived from alfalfa crops.

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Increased copper levels inhibit denitrification in urban soils

Cited by 7 publications

References 43 publications

Hydrological Perturbations Facilitated Phyllosphere Denitrification of an Urban Greening Tree

Hydrological Perturbations Facilitated Phyllosphere Denitrification of an Urban Greening Tree

Increasing the tolerance to copper of ornamental plants and lawn grasses in urban ecosystems

Ensifer meliloti denitrification is involved in infection effectiveness and N2O emissions from alfalfa root nodules

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