Increased CYR61 expression activates CCND1/c-Myc pathway to promote nasal epithelial cells proliferation in chronic rhinosinusitis with nasal polyps

Luo, Chunyu; Zhu, Ying; Zhou, Jiayao; Sun, Xiaochun; Zhang, Shiyao; Tan, Shaolin; Li, Zhipeng; Lin, Hai; Zhang, Weitian

doi:10.1016/j.clim.2023.109235

Cited by 4 publications

(3 citation statements)

References 42 publications

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“…5E). In the nasal epithelium Myc together with Ccn1 and Ccnd1 induce cell proliferation 90 . This suggests that mutant cells are defective in cell-cycle regulation (Fig.…”

Section: Resultsmentioning

confidence: 99%

Postnatal FGFR-signaling establishes gradients of secretory cell identities along the proximal-distal axis of the lung airways

Sountoulidis,

Firsova,

Liontos

et al. 2023

Preprint

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Secretory cells are major structural and functional constituents of the lung airways. Their spatial organization and specification mechanisms are partially understood. Here, we labelled major secretory airway cell types and analysed them at single-cell resolution. We found opposing, partially overlapping gene-expression gradients along the proximal-distal airway axis superimposed on a general gene program encoding detoxification. One graded program is elevated proximally and relates to innate immunity, whereas the other is enriched distally, encoding lipid metabolism and antigen presentation. Intermediately positioned cells express low levels of both graded programs and show increased clonogenic potency in vitro, relating cell-plasticity to location in each branch. Single-cell RNA-sequencing following lineage-tracing revealed the sequential and postnatal establishment of the gradients in common epithelial progenitors. Fgfr2b is distally enriched and its postnatal inactivation reduces distal gene expression and expands proximal genes into distally located cells. This suggests a central role of FGFR-signaling in tissue-scale airway patterning.

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“…5E). In the nasal epithelium Myc together with Ccn1 and Ccnd1 induce cell proliferation 90 . This suggests that mutant cells are defective in cell-cycle regulation (Fig.…”

Section: Resultsmentioning

confidence: 99%

Postnatal FGFR-signaling establishes gradients of secretory cell identities along the proximal-distal axis of the lung airways

Sountoulidis,

Firsova,

Liontos

et al. 2023

Preprint

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show abstract

“…HNEpC, situated on the nasal cavity's inner surface, function as a primary defense against inhaled pathogens, particles, and irritants 7 , 16 . Interaction between LPS and HNEpC in nasal mucosal tissues triggers immune responses and inflammatory reactions.…”

Section: Discussionmentioning

confidence: 99%

“…Nasal epithelial cells, forming the outermost barrier of nasal mucosal tissue, are the primary targets of external pathogenic agents such as bacteria, viruses, and environmental pollutants. This epithelial barrier plays a crucial role in preventing the entry of harmful substances and microorganisms into the lower respiratory tracts, including the bronchi and lungs 7 , 8 . Numerous studies have demonstrated that external bacteria can invade nasal epithelial cells, compromising the epithelial barrier and triggering a cascade of inflammatory and immune responses 9 , 10 .…”

Section: Introductionmentioning

confidence: 99%

Insights from bioinformatics analysis reveal that lipopolysaccharide induces activation of chemokine-related signaling pathways in human nasal epithelial cells

Tan,

Gu,

Zhu

et al. 2024

Sci Rep

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Lipopolysaccharide (LPS) is known to elicit a robust immune response. This study aimed to investigate the impact of LPS on the transcriptome of human nasal epithelial cells (HNEpC). HNEpC were cultured and stimulated with LPS (1 μg/mL) or an equivalent amount of normal culture medium. Subsequently, total RNA was extracted, purified, and sequenced using next-generation RNA sequencing technology. Differentially expressed genes (DEGs) were identified and subjected to functional enrichment analysis. A protein–protein interaction (PPI) network of DEGs was constructed, followed by Ingenuity Pathway Analysis (IPA) to identify molecular pathways influenced by LPS exposure on HNEpC. Validation of key genes was performed using quantitative real-time PCR (qRT-PCR). A total of 97 DEGs, comprising 48 up-regulated genes and 49 down-regulated genes, were identified. Results from functional enrichment analysis, PPI, and IPA indicated that DEGs were predominantly enriched in chemokine-related signaling pathways. Subsequent qRT-PCR validation demonstrated significant upregulation of key genes in these pathways in LPS-treated HNEpC compared to control cells. In conclusion, LPS intervention profoundly altered the transcriptome of HNEpC, potentially exacerbating inflammatory responses through the activation of chemokine-related signaling pathways.

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The mucosal concept in chronic rhinosinusitis: Focus on the epithelial barrier

Yan,

Lan,

et al. 2024

Journal of Allergy and Clinical Immunology

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Increased CYR61 expression activates CCND1/c-Myc pathway to promote nasal epithelial cells proliferation in chronic rhinosinusitis with nasal polyps

Cited by 4 publications

References 42 publications

Postnatal FGFR-signaling establishes gradients of secretory cell identities along the proximal-distal axis of the lung airways

Postnatal FGFR-signaling establishes gradients of secretory cell identities along the proximal-distal axis of the lung airways

Insights from bioinformatics analysis reveal that lipopolysaccharide induces activation of chemokine-related signaling pathways in human nasal epithelial cells

The mucosal concept in chronic rhinosinusitis: Focus on the epithelial barrier

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