Increased degradation of insulin-like growth factor-I in serum from feed-deprived steers

Wu, Mingquan; Wang, Aihua; Bernard, Gregory C.; Hall, J. B.; Beal, W. E.; Akers, R.M.; Boisclair, Yves R.; Jiang, Honglin

doi:10.1016/j.domaniend.2008.07.003

Cited by 8 publications

(10 citation statements)

References 68 publications

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“…These cDNA plasmids are described in detail in a previous paper (Wu et al 2008). The RPA was carried out using the RPA II kit (Ambion, Austin, TX, USA) as described previously (Wu et al 2008). Briefly, 20 mg of total RNA were hybridized with 1!10 5 dpm of the probe for IGF1, ALS, or IGFBP3 mRNA, and with 1!10 5 dpm of the probe for GAPDH mRNA at 42 8C overnight.…”

Section: Ribonuclease Protection Assaymentioning

confidence: 99%

Section: Ribonuclease Protection Assaymentioning

confidence: 99%

“…The serum samples for GH RIA were not extracted. In the IGF1 RIA, 100 ml of each sample in duplicate were used; the IGF1 standard used was recombinant human IGF1 (GroPep, Adelaide, SA, Australia); the primary antibody used was a mouse anti-human IGF1 antibody previously validated for bovine IGF1 (Berry et al 2003, Wu et al 2008. In the GH RIA, 300 ml of each sample in duplicate were used; the GH standard used was a recombinant bovine GH; the primary antibody used was a rabbit anti-ovine GH antibody previously validated for bovine GH (Barnes et al 1985).…”

Section: Riamentioning

confidence: 99%

“…This analysis was used to quantify ALS protein in the serum samples, and was carried out as described previously (Wu et al 2008). Briefly, 0 .…”

Section: Western Blot Analysismentioning

confidence: 99%

“…RIA was used to determine serum concentrations of total IGF1 and GH, and was done as described previously (Berry et al 2003, Wu et al 2008). An acid-ethanol cryoprecipitation extraction was performed using the serum samples for IGF1 RIA to remove the IGFBPs.…”

Section: Riamentioning

confidence: 99%

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Effect of feeding level on serum IGF1 response to GH injection

Hall²,

Akers³

et al. 2010

Journal of Endocrinology

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This study was conducted to further understand the mechanism by which nutrition modulates GH-induced changes in serum IGF1 concentration in cattle. Cows were fed hay only or corn-based concentrates in addition to hay for 8 weeks. At week 8, serum concentrations of IGF1, IGF-binding protein 3 (IGFBP3), and acid-labile subunit (ALS) as well as their mRNA levels in liver were determined immediately before and 7 days after an injection of GH formulated for sustained release. GH injection caused greater increases in both serum IGF1 concentration and liver IGF1 mRNA expression in the cows fed concentrates than in those fed hay. In the cows fed concentrates, the magnitude of the GH-induced increase in serum IGF1 concentration was, however, much greater than that in liver IGF1 mRNA expression. This difference was not due to GH-induced IGF1 mRNA being translated more efficiently. GH injection also induced greater increases in serum IGFBP3 and ALS concentrations in the cows fed concentrates than fed hay. The injection, however, did not induce a greater increase in IGFBP3 or ALS mRNA in the cows fed concentrates than fed hay. These data and the fact that the ternary complex of IGF1/IGFBP3/ALS stabilizes each of the components in the blood suggest that in cows, increased nutrition enhances serum IGF1 response to GH not only by increasing IGF1 mRNA response to GH in the liver, but also by elevating IGFBP3 and ALS concentrations in the blood.

show abstract

Section: Ribonuclease Protection Assaymentioning

confidence: 99%

Section: Ribonuclease Protection Assaymentioning

confidence: 99%

Section: Riamentioning

confidence: 99%

“…This analysis was used to quantify ALS protein in the serum samples, and was carried out as described previously (Wu et al 2008). Briefly, 0 .…”

Section: Western Blot Analysismentioning

confidence: 99%

Section: Riamentioning

confidence: 99%

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Effect of feeding level on serum IGF1 response to GH injection

Hall²,

Akers³

et al. 2010

Journal of Endocrinology

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Effects of road transportation or droving on the weight and metabolism of young bulls

D’Oliveira

Souza

Filho

et al. 2014

Trop Anim Health Prod

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This study evaluated the effects of the mode of transportation on weight and metabolism of young bulls, which are generally transported by two systems: droving and truck. This experiment consisted of two phases: transportation and refeeding and involved transportation by droving and truck. Fifty young Nellore bulls were separated in equal numbers in both groups, droving group and truck group. In the transportation phase, animals were moved, in a 640-km journey. The refeeding phase consisted of 84 days and commenced after the animals arrived at the fattening farm. The effect of the transportation system on animals' weight, metabolic hormones, and serum biochemistry was assessed after 28, 56, and 84 days. During the transportation phase, animals in the droving group lost 10.11 kg and animals in the truck group gained 13.1 kg. The truck group showed 20.5 kg of weight gain at the end of the refeeding phase. The highest triiodothyronine (T3) and thyroxine (T4) concentrations were observed on days 0 and 60 in the transportation phase in both droving and truck groups. The serum cortisol concentration was higher in the droving group than in the truck group on day 56 of the refeeding phase. The IGF-I concentrations were higher during the refeeding phase in the droving group than in the truck group. Our results showed that transportation by droving leads to increased weight loss and reduced further weight gain compared to transportation by truck and that serum concentrations of T4, cortisol, and insulin-like growth factor (IGF-I) hormones are altered by the transportation system.

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Growth hormone stimulation of serum insulin concentration in cattle: Nutritional dependency and potential mechanisms

Feng

et al. 2009

Domestic Animal Endocrinology

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Increased degradation of insulin-like growth factor-I in serum from feed-deprived steers

Cited by 8 publications

References 68 publications

Effect of feeding level on serum IGF1 response to GH injection

Effect of feeding level on serum IGF1 response to GH injection

Effects of road transportation or droving on the weight and metabolism of young bulls

Growth hormone stimulation of serum insulin concentration in cattle: Nutritional dependency and potential mechanisms

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