Increased detection of prolylaminopeptidase in Neisseria meningitidis by Identicult-Neisseria

Sperry, J. F.; Cohenford, Menashi A.; Campognone, Paul; Lawton, William D.; Chee, D O

doi:10.1128/jcm.24.1.145-.1986

Cited by 11 publications

(3 citation statements)

References 6 publications

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“…We found that 26.3'X of the N . meningitidis strains were positive for both GAP and PAP; this is considerably lower than the 92.5% reported by Sperry et al (1986) who advocated that N . meningitidis strains be regarded as PAP-positive.…”

Section: Discussioncontrasting

confidence: 60%

Rapid identification of pathogenic neisserias using the Identicult-Neisseria test

Moyes

Chronas

Young³

1994

Lett Appl Microbiol

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A rapid enzymatic method using chromogenic substrates for the rapid identification of pathogenic neisseria (Identicult-Neisseria, Scott Laboratories Inc., CA, USA) was tested in parallel with the rapid carbohydrate utilization test (RCUT) and the Phadebact Monoclonal GC Test against 198 consecutive clinical isolates of oxidase-positive Gram-negative diplococci (118 Neisseria gonorrhoeae, 76 N. meningitidis and four N. lactamica). On initial testing the Identicult-Neisseria gave a 95% overall concordance (97.5% N. gonorrhoeae, 90.8% N. meningitidis) with the RCUT and Phadebact tests; the corresponding figures after repeat testing were 98% overall concordance (98.3% N. gonorrhoeae, 97.4% N. meningitidis). Two of the three strains of N. gonorrhoeae mis-identified as N. meningitidis on primary testing were also mis-identified on repeat testing. Seven strains of N. meningitidis were mis-identified on initial testing (six as Moraxella catarrhalis and one as N. lactamica) and two on repeat testing (both as Mor. catarrhalis). We conclude that the Identicult-Neisseria is not sufficiently reliable for the culture confirmation of gonococci and meningococci.

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Section: Discussioncontrasting

confidence: 60%

Rapid identification of pathogenic neisserias using the Identicult-Neisseria test

Moyes

Chronas

Young³

1994

Lett Appl Microbiol

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“…Methods for the identification of Neisseria spp. include carbohydrate degradation tests (8,13), chromogenic enzyme substrate tests (6,7,16,17), and combinations of these two approaches (14,15). Conventional procedures use cystinetryptic digest agar (CTA) base medium with added carbohydrates and may require prolonged incubation for some isolates before results are available (13).…”

mentioning

confidence: 99%

“…Most recently, identification methods with specific chromogenic enzyme substrates have become available for rapid identification of Neisseria spp. that grow on selective medium (i.e., N. gonorrhoeae, N. meningitidis, and N. lactamica) (6,7,14,16,17). These systems also provide a presumptive identification of Branhamella catarrhalis, an organism of recognized clinical importance that can also be occasionally recovered on selective medium (e.g., modified Thayer-Martin [MTMI and Martin-Lewis agars) (4; G. V. Doern, Clin.…”

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confidence: 99%

API QuadFERM+ with rapid DNase for identification of Neisseria spp. and Branhamella catarrhalis

Janda¹,

Zigler²,

Bradna³

1987

J Clin Microbiol

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The QuadFERM+ system (Analytab Products, Plainview, N.Y.), a 2-h carbohydrate degradation method for the identification of Neisseria spp., was evaluated along with a rapid DNase test for confirmation of Branhamella catarrhalis. QuadFERM+ identified 100% of 82 N. gonorrhoeae and 96% of 54 N. meningitidis strains. The two misidentified meningococcal strains were biochemically atypical and were also misidentified by the conventional method. Of 26 N. lactamica strains, 25 (96%) were correctly identified. Of 21 Neisseria spp., 14 (67%) produced carbohydrate reactions in agreement with the conventional procedure, and 7 strains produced detectable acid in the QuadFERM + from maltose and sucrose but not glucose. All 9 N. cinerea and 30 B. catarrhalis strains were asaccharolytic by QuadFERM+. The rapid DNase test was positive for all B. catarrhalis strains and negative for all other organisms. Two beta-lactamase-positive N. gonorrhoeae strains and 25 (93%) of 27 beta-lactamase-positive B. catarrhalis strains were detected by the 2-h acidometric beta-lactamase test on the strip. QuadFERM+ with rapid DNase is a simple and easily interpretable method for identification of these organisms in the clinical laboratory.

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Evaluation of a ten-minute chromogenic substrate test for identification of pathogenicNeisseria species andBranhamella catarrhalis

Janda

Sobieski²

1988

Eur. J. Clin. Microbiol. Infect. Dis.

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A ten-minute chromogenic substrate test was evaluated for its ability to rapidly identify pathogenic Neisseria spp. and Branhamella catarrhalis. Identifications obtained with this system were compared to those obtained using conventional procedures. The test correctly identified 98.9% of 90 Neisseria gonorrhoeae, 98.3% of 60 Neisseria meningitidis, 96.2% of 26 Neisseria lactamica, and 100% of 36 Branhamella catarrhalis strains. Eight Neisseria subflava strains that grew on modified Thayer-Martin agar were prolyl aminopeptidase positive and were misidentified as Neisseria gonorrhoeae. Other strains of saprophytic Neisseria spp. also reacted with the chromogenic substrates. The system was accurate and reliable for identifying the commonly encountered pathogenic species. In light of recent reports describing new species and atypical Neisseria strains, however, careful attention to the salient features of both common and atypical organisms is necessary for proper use of rapid enzymatic identification tests.

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Increased detection of prolylaminopeptidase in Neisseria meningitidis by Identicult-Neisseria

Cited by 11 publications

References 6 publications

Rapid identification of pathogenic neisserias using the Identicult-Neisseria test

Rapid identification of pathogenic neisserias using the Identicult-Neisseria test

API QuadFERM+ with rapid DNase for identification of Neisseria spp. and Branhamella catarrhalis

Evaluation of a ten-minute chromogenic substrate test for identification of pathogenicNeisseria species andBranhamella catarrhalis

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