Increased ethanol production by deletion of <i>HAP4</i> in recombinant xylose-assimilating <i>Saccharomyces cerevisiae</i>

Matsushika, Akinori; Hashizume, Tamotsu

doi:10.1007/s10295-015-1695-5

Cited by 8 publications

(2 citation statements)

References 29 publications

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“…To improve the production of ethanol by a xylose-utilizing strain of S. cerevisiae, the HAP4 gene was knocked out. 159 This gene encodes a transcription activator that controls expression of genes involved in mitochondrial respiration and reductive pathways. By knocking out the HAP4 gene, the following were increased: maximal ethanol concentration, ethanol production rate and ethanol yield.…”

Section: Biofuelsmentioning

confidence: 99%

Production of valuable compounds by molds and yeasts

Demain

Martens

2016

J Antibiot

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where he has contributed in a major way to the reputation of this department for many years. He also served as an Adjunct Professor at the University of Geneva. Among Julian's areas of study and accomplishment are fungal toxins including α-sarcin, chemical synthesis of triterpenes, mode of action of streptomycin and other aminoglycoside antibiotics, biochemical mechanisms of antibiotic resistance in clinical isolates of bacteria harboring resistance plasmids, their origins and evolution, secondary metabolism of microorganisms, structure and function of bacterial ribosomes, antibiotic resistance mutations in yeast ribosomes, cloning of resistance genes from an antibiotic-producing microbe, gene cloning for industrial purposes, engineering of herbicide resistance in useful crops, bleomycin-resistance gene in clinical isolates of Staphylococcus aureus and many other topics. He has been an excellent teacher, lecturing in both English and French around the world, and has organized international courses. Julian has also served on the NIH study sections, as Editor for several international journals, and was one of the founders of the journal Plasmid. We expect the impact of Julian's accomplishments to continue into the future.

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Section: Biofuelsmentioning

confidence: 99%

Production of valuable compounds by molds and yeasts

Demain

Martens

2016

J Antibiot

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“…Finally, the hydrolysate is used by microorganisms as a carbon source during fermentation. With this process, biofuels, such as 2,3-butanediol [12], ethanol [13] and isobutanol [14], are being produced by wild-type or genetically engineered microorganisms. However, a number of challenges remain, including the need to reduce production costs, which have made it difficult to put these methods to industrial use.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Saccharification and Fermentation for Isobutanol Production from Banana Peel

Akita,

Shibata,

Komoriya

et al. 2024

Fermentation

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Each year, near 40 million tons of banana peels are discarded around the world. This plant biomass could potentially be utilized for energy production. Simultaneous saccharification and fermentation (SSF) is an effective method for producing biofuels from plant biomasses. Since SSF with enzymatic hydrolysis and fermentation are performed simultaneously in the same reactor, the production process is simpler than most existing methods. Here, we describe isobutanol production using SSF with hydrothermally treated banana peel samples and an Escherichia coli strain able to utilize glucose and xylose to produce isobutanol. To enhance the glucose and xylose concentrations, the reaction conditions for the enzymatic hydrolysis of plant biomass using two kinds of saccharification enzymes were optimized, including the enzyme unit ratio, reaction temperature and sample gram. When the optimized conditions for enzymatic hydrolysis were applied to SSF, the glucose and xylose produced from the hydrothermally treated samples were consumed, producing isobutanol. Moreover, the isobutanol concentration increased with an increasing initial culture pH, reaching 1.27 g/L at pH 6.5, which was consistent with the optimal initial culture pH for isobutanol production by this E. coli strain. Taken together, these results indicate that the established method is potentially useful for industrial isobutanol production.

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