Increased expression and altered intracellular distribution of adhesion/growth‐regulatory lectins galectins‐1 and ‐7 during tumour progression in hypopharyngeal and laryngeal squamous cell carcinomas

Saussez, Sven; Decaestecker, Christine; Lorfevre, Francois; Chevalier, Dominique; Mortuaire, G.; Kaltner, Herbert; André, Sabine; Toubeau, Gérard; Gabius, Hans‐Joachim; Leroy, Xavier

doi:10.1111/j.1365-2559.2008.02973.x

Cited by 60 publications

(71 citation statements)

References 34 publications

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“…Its overexpression has been correlated with several aspects of cancer biology, including modulation of cancer cells apoptosis, migration and adhesion (21). Gal-1 was found overexpressed in hypopharyngeal and laryngeal squamous cell carcinomas (9). It also increased the adhesion of prostate cancer cells and ovarian cancer cells to extracellular matrix (22).…”

Section: Discussionmentioning

confidence: 95%

Increased expression of galectin-1 is associated with human oral squamous cell carcinoma development

Ding

Dong²,

Chen³

et al. 2009

Oncol Rep

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Abstract. Galectin-1 (Gal-1) is a newly found immunoregulatory carbohydrate-binding protein in cancer biology. The purpose of this study was to evaluate the effects of Gal-1 on oral squamous cell carcinoma (OSCC) development. Immuno-histochemistry, Western blotting and RT-PCR were carried out in 62 primary OSCC, 38 oral leukoplakia (OPL) tissues to detect the Gal-1 expression in both protein and mRNA levels. Ten normal oral mucosa (NOM) tissues were used as control. Gal-1 protein was significantly overexpressed in OSCC cancer cells and OPL prickle cells compared to NOM (P<0.05). In accordance with Gal-1 protein, Gal-1 mRNA was also up-regulated in OSCC tissues and OPL tissues. Furthermore, both the Gal-1 protein and mRNA in OSCC tissues were higher than in OPL tissues (P<0.05). Our data supports the important roles of Gal-1 in OSCC development and suggests that Gal-1 upregulation in the OSCC and OPL tissues might be a predictor of early oral carcinogenesis.

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Section: Discussionmentioning

confidence: 95%

Increased expression of galectin-1 is associated with human oral squamous cell carcinoma development

Ding

Dong²,

Chen³

et al. 2009

Oncol Rep

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“…Moreover, its presence is also seen in the cytosol of neuroblastoma and small cell lung carcinoma tissues, testicular interstitial and cervical carcinoma cell lines (MA-10 and HeLa), hypopharyngeal (HSCCs) and laryngeal (LSCCs) squamous cell carcinoma tissues, human melanoma cell lines (A375 and A2058) and colorectal cancer tissues including adenomas, carcinomas and metastases from patients (9,(11)(12)(13)(14)(15). Although fewer studies have been conducted on galectin-2, the available data indicate its presence in the nucleus of genetically engineered human colon cancer cells that have ectopic stable expression (16) in addition to gastric carcinoma tissues, epidermoid carcinoma, osteosarcoma and glioblastoma cell lines (A-431, U-2 OS and U-251MG) (17,18).…”

Section: Where Do We Find Galectins Inside the Cells?mentioning

confidence: 99%

“…Galectin-4 is detected in the cytosol of human breast ductal carcinoma tissues (28,29) and pancreatic adenocarcinoma cell line (Pa-Tu-8988S) (29) as well as inside the basal plasma membrane of human colon adenocarcinoma cells (T84) (27). Galectin-7, which has recently attracted more interest in cancer because its preferential expression in epithelial tissues and carcinomas, is seen in the nucleus of many cancer cells, including hypopharyngeal (HSCCs) and laryngeal (LSCCs) squamous cell carcinomas tissues, colon carcinoma cells (DLD-1), cervical adenocarcinoma (HeLa), epithelial ovarian cancer tissues and oral epithelial dysplasia tissues (13,(30)(31)(32). Galectin-7 is also observed in the cytosol of the colon carcinoma cell line DLD-1, cervical adenocarcinoma cells (HeLa), epithelial ovarian cancer and oral epithelial dysplasia tissues (17,(30)(31)(32).…”

Section: Where Do We Find Galectins Inside the Cells?mentioning

confidence: 99%

Intracellular galectins in cancer cells: Potential new targets for therapy

Vladoiu

Labrie

St‐Pierre

2014

International Journal of Oncology

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Abstract. Dysregulation of galectin expression is frequently observed in cancer tissues. Such an abnormal expression pattern often correlates with aggressiveness and relapse in many types of cancer. Because galectins have the ability to modulate functions that are important for cell survival, migration and metastasis, they also represent attractive targets for cancer therapy. This has been well-exploited for extracellular galectins, which bind glycoconjugates expressed on the surface of cancer cells. Although the existence of intracellular functions of galectins has been known for many years, an increasing number of studies indicate that these proteins can also alter tumor progression through their interaction with intracellular ligands. In fact, in some instances, the interactions of galectins with their intracellular ligands seem to occur independently of their carbohydrate recognition domain. Such findings call for a change in the basic assumptions, or paradigms, concerning the activity of galectins in cancer and may force us to revisit our strategies to develop galectin antagonists for the treatment of cancer.

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“…All tumor samples were fixed in 4% buffered formaldehyde for 24 h, dehydrated and embedded in paraffin. Immunohistochemistry was performed on 5-µm thick sections mounted on silane-coated glass slides (25). Before starting the immunohistochemistry protocol, deparaffinized tissue sections were placed in a 0.01 M citrate buffer (pH 6.0) and briefly pre-treated in a 900-W microwave for 2x5 min.…”

Section: Realmentioning

confidence: 99%

High incidence of high-risk HPV in benign and malignant lesions of the larynx

Duray

Descamps²,

Arafa³

et al. 2011

Int J Oncol

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Abstract. The aim of this study was to determine the prevalence of human papillomavirus (HPV) in patients with laryngeal benign lesions (LBLs) and laryngeal squamous cell carcinomas (LSCCs) using a sensitive E6/E7 type-specific PCR. Paraffinembedded samples from LBL (n=39) and LSCC patients (n=67) were evaluated for the presence of HPV DNA by GP5 + /GP6 + consensus PCR and E6/E7 type-specific PCR for HPV types 6,11,16,18,31,33,35, 39, 45, 51, 52, 53, 56, 58, 59, 66 and 68. In LSCCs, immunohistochemical staining of p16, p53 and EGFR was also assessed. The E6/E7 type-specific PCR showed that 44 out of 59 LSCC patients (i.e., 75%) had high-risk (hr) HPV types and that 27 out of 35 LBL patients (i.e., 77%) had hrHPV types. HPV-16 viral load was significantly higher in LSCC than in LBL patients (p<10 -6 ). The presence of hrHPV DNA did not correlate with the proportion of disease-free patients. Comparable levels of p16, p53 and EGFR expression were observed in the hrHPV + tumor group (100% p16 + , 56% p53 + and 97% EGFR + ) and in the HPV -or low-risk (lr) HPV + tumor group (92% p16 + , 66% p53 + and 100% EGFR + ). A very high prevalence of oncogenic HPV-16 was found in a series of benign and malignant laryngeal lesions. LSCC appears to be characterized by an active hrHPV infection. In LSCCs, the hrHPV + subgroup had a similar prognosis (in terms of risk of recurrence) as the HPV -subgroup.

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Increased expression and altered intracellular distribution of adhesion/growth‐regulatory lectins galectins‐1 and ‐7 during tumour progression in hypopharyngeal and laryngeal squamous cell carcinomas

Cited by 60 publications

References 34 publications

Increased expression of galectin-1 is associated with human oral squamous cell carcinoma development

Increased expression of galectin-1 is associated with human oral squamous cell carcinoma development

Intracellular galectins in cancer cells: Potential new targets for therapy

High incidence of high-risk HPV in benign and malignant lesions of the larynx

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