Increased expression of clp genes in Lactobacillus delbrueckii UFV H2b20 exposed to acid stress and bile salts

Ferreira, Alessandra Barbosa; Oliveira, Marcelo Nagem Valério de; Freitas, Fernanda; Alfenas‐Zerbini, Poliane; Silva, D.F. Da; Queiroz, Marisa Vieira de; Borges, Arnaldo Chaer; Moraes, Célia Alencar de

doi:10.3920/bm2013.0022

Cited by 23 publications

(14 citation statements)

References 20 publications

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“…Exposure of Lb. delbrueckii to 0.1% bile salts increased the expression of clpP and clpE, which, as we have seen above, are involved in repair/degradation of damaged proteins (466). This is in agreement with the observation that mutations in lr1864 (clpL) in Lb.…”

Section: Stress Associated With Intestinal Transitsupporting

confidence: 80%

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Stress Physiology of Lactic Acid Bacteria

Papadimitriou

Alegría

Bron

et al. 2016

Microbiol Mol Biol Rev

539

404

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SUMMARYLactic acid bacteria (LAB) are important starter, commensal, or pathogenic microorganisms. The stress physiology of LAB has been studied in depth for over 2 decades, fueled mostly by the technological implications of LAB robustness in the food industry. Survival of probiotic LAB in the host and the potential relatedness of LAB virulence to their stress resilience have intensified interest in the field. Thus, a wealth of information concerning stress responses exists today for strains as diverse as starter (e.g.,Lactococcus lactis), probiotic (e.g., severalLactobacillusspp.), and pathogenic (e.g.,EnterococcusandStreptococcusspp.) LAB. Here we present the state of the art for LAB stress behavior. We describe the multitude of stresses that LAB are confronted with, and we present the experimental context used to study the stress responses of LAB, focusing on adaptation, habituation, and cross-protection as well as on self-induced multistress resistance in stationary phase, biofilms, and dormancy. We also consider stress responses at the population and single-cell levels. Subsequently, we concentrate on the stress defense mechanisms that have been reported to date, grouping them according to their direct participation in preserving cell energy, defending macromolecules, and protecting the cell envelope. Stress-induced responses of probiotic LAB and commensal/pathogenic LAB are highlighted separately due to the complexity of the peculiar multistress conditions to which these bacteria are subjected in their hosts. Induction of prophages under environmental stresses is then discussed. Finally, we present systems-based strategies to characterize the “stressome” of LAB and to engineer new food-related and probiotic LAB with improved stress tolerance.

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Section: Stress Associated With Intestinal Transitsupporting

confidence: 80%

“…delbrueckii cells to acidic conditions prevailing in the GIT (pH 3.5 for 60 min) was shown to result in increased expression of the clpP, clpE, clpL, and clpX genes. As detailed above, the Clp chaperones actively promote refolding or degradation of damaged proteins (466).…”

Section: Stress Associated With Intestinal Transitmentioning

confidence: 99%

Stress Physiology of Lactic Acid Bacteria

Papadimitriou

Alegría

Bron

et al. 2016

Microbiol Mol Biol Rev

539

404

View full text Add to dashboard Cite

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“…; Alcántara and Zúñiga ; Ferreira et al . ). Tween 80 and prebiotics were added to Lactococcus and Lactobacillus cultures to enhance bile resistance for application purposes (Kimoto et al .…”

Section: Resultsmentioning

confidence: 97%

“…Previous studies have applied the bile stress method or molecular biological method to reveal the mechanism of enhanced bile resistance (Noriega et al 2004;Whitehead et al 2008;Hamon et al 2011;Alc antara and Z uñiga 2012;Ferreira et al 2013). Tween 80 and prebiotics were added to Lactococcus and Lactobacillus cultures to enhance…”

Section: Effects Of Soy Lecithin On Strain Bile Resistancementioning

confidence: 99%

Enhancement of bile resistance in Lactobacillus plantarum strains by soy lecithin

Tian

Wang

et al. 2015

Lett Appl Microbiol

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In this study, we report for the first time the beneficial effect of adding soy lecithin to an MRS broth on subsequent bile tolerance of Lactobacillus plantarum. Soy lecithin had no inhibitory effect on strain viability but significantly enhanced bile resistance. Surface hydrophobicity and cell integrity increased in strains cultured with soy lecithin. The observed shift in the cell fatty acid composition indicated changes to the cell membrane. As soy lecithin is safe for use in the food industry, its protective effects can be harnessed for the development of bile-sensitive strains with health-benefit functions for use in probiotic products.

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“…The substrate specificity of ClpP is often determined by the regulatory ClpP subunits, and the ClpP subunits alone can function as chaperones. The Clp proteases are found also in several LAB species, and they are important for the survival of some LAB species, including L. casei (33), L. delbrueckii (34), and L. reuteri (35), in acid stress and bile salts. Furthermore, Clp proteases are associated with thermotolerance of the species Strep- (36,37).…”

Section: Discussionmentioning

confidence: 99%

Adaptation of Lactobacillus plantarum to Ampicillin Involves Mechanisms That Maintain Protein Homeostasis

et al. 2020

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The widespread use of antibiotics has caused great concern in the biosafety of probiotics. In this study, we conducted a 12-month adaptive laboratory evolution (ALE) experiment to select for antibiotics-adapted Lactobacillus plantarum P-8, a dairy-originated probiotic bacterium. During the ALE process, the ampicillin MIC for the parental L. plantarum P-8 strain increased gradually and reached the maximum level of bacterial fitness. To elucidate the molecular mechanisms underlying the ampicillin-resistant phenotype, we comparatively analyzed the genomes and proteomes of the parental strain (L. plantarum P-8) and two adapted lines (L. plantarum 400g and L. plantarum 1600g). The adapted lines showed alterations in their carbon, amino acid, and cell surface-associated metabolic pathways. Then, gene disruption mutants were created to determine the role of six highly expressed genes in contributing to the enhanced ampicillin resistance. Inactivation of an ATP-dependent Clp protease/the ATP-binding subunit ClpL, a small heat shock protein, or a hypothetical protein resulted in partial but significant phenotypic reversion, confirming their necessary roles in the bacterial adaptation to ampicillin. Genomic analysis confirmed that none of the ampicillin-specific differential expressed genes were flanked by any mobile genetic elements; thus, even though long-term exposure to ampicillin upregulated their expression, there is low risk of spread of these genes and adapted drug resistance to other bacteria via horizontal gene transfer. Our study has provided evidence of the biosafety of probiotics even when used in the presence of antibiotics. IMPORTANCE Antibiotic resistance acquired by adaptation to certain antibiotics has led to growing public concerns. Here, a long-term evolution experiment was used together with proteomic analysis to identify genes/proteins responsible for the adaptive phenotype. This work has provided novel insights into the biosafety of new probiotics with high tolerance to antibiotics.

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Increased expression of clp genes in Lactobacillus delbrueckii UFV H2b20 exposed to acid stress and bile salts

Cited by 23 publications

References 20 publications

Stress Physiology of Lactic Acid Bacteria

Stress Physiology of Lactic Acid Bacteria

Enhancement of bile resistance in Lactobacillus plantarum strains by soy lecithin

Adaptation of Lactobacillus plantarum to Ampicillin Involves Mechanisms That Maintain Protein Homeostasis

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