2004
DOI: 10.1002/jcp.20259
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Increased expression of cystic fibrosis transmembrane conductance regulator in rat liver after common bile duct ligation

Abstract: Liver disease associated with cystic fibrosis (CF) has been increasingly diagnosed and recognized as one of the major causes of death in CF during recent years. The autosomal-recessive disorder of CF results from mutation of the cystic fibrosis transmembrane conductance regulator (CFTR) that encodes the CFTR protein. Due to its existence and multifunction in biliary epithelial, over- or less-expression of CFTR in the liver may play an important role in the development of CF liver disease (CFLD). The aim of cur… Show more

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Cited by 22 publications
(15 citation statements)
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“…In the patient with biliary atresia, intense ductular reaction was associated with increased hepatic mRNA levels of CFTR, as previously reported in experimental bile duct obstruction. 37 In the patient with PFIC2 who had no ductular reaction, CFTR mRNA levels were unchanged. By contrast, CFTR mRNA levels were profoundly decreased in both patients with PFIC1, irrespective of whether they had or not bile duct proliferation.…”
Section: Discussionmentioning
confidence: 97%
“…In the patient with biliary atresia, intense ductular reaction was associated with increased hepatic mRNA levels of CFTR, as previously reported in experimental bile duct obstruction. 37 In the patient with PFIC2 who had no ductular reaction, CFTR mRNA levels were unchanged. By contrast, CFTR mRNA levels were profoundly decreased in both patients with PFIC1, irrespective of whether they had or not bile duct proliferation.…”
Section: Discussionmentioning
confidence: 97%
“…The cell lysate containing 20 μg of total protein was separated on 10% sodium dodecyl sulfate-polyacrylamide (SDS-polyacrylamide) gel under reducing conditions, and transferred onto polyvinylidene [29]. For equal loading test, the blotted membranes were stripped and then re-probed with the primary antibody against β-actin.…”
Section: Western Blotmentioning
confidence: 99%
“…Anti-IGF-1R monoclonal antibody (1:1,000, 38 kDa; Abcam, Cambridge, Mass., USA), and anti-MRP-1 monoclonal antibody (1:1,000, 190 kDa; Abcam) were used in primary reactions. Bands were visualized by employing the enhanced chemiluminescence method [25]. Human β-actin was used as an internal control [25, 26], As expected, a single band of them was detected (fig.…”
Section: Methodsmentioning
confidence: 99%
“…Bands were visualized by employing the enhanced chemiluminescence method [25]. Human β-actin was used as an internal control [25, 26], As expected, a single band of them was detected (fig. 1).…”
Section: Methodsmentioning
confidence: 99%