Increased Expression of E-cadherin, Endothelin-1, and CD68 in Preeclamptic Placentas

İrtegün, Sevgi; Tekin, Mehmet; Alpayci, Rojbin

doi:10.5152/etd.2016.0090

Cited by 5 publications

(6 citation statements)

References 22 publications

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“…Contrary to the result of present study, some studies have reported that placental ET-1 expression was significantly higher in preeclamptic placentae than in normal placentae, 12 13 whereas other studies have reported that ET-1 mRNA expression was significantly lower in preeclamptic placentae than in normal placentae. 14 In these studies, placental ET-1 mRNA expression was determined by northern-blot analysis, 12 real-time polymerase chain reaction (RT-PCR) 14 and western blot.…”

Section: Discussioncontrasting

confidence: 99%

“…11 However, there are still controversial findings concerning the level of placental ET-1 in normal pregnant women and those with PE. Studies have found that the levels of tissue endothelin in placental tissues were higher, 12 13 lower, 14 or not significantly different 8 15 16 in preeclamptic pregnant women compared with normal pregnant women.…”

Section: Introductionmentioning

confidence: 98%

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Expression of Endothelin-1 and Endothelial Nitric Oxide Synthase in Normal and Preeclamptic Placentae

Khaing

Swe

Aung

et al. 2022

Rev Bras Ginecol Obstet

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Objective To investigate the expression of endothelin-1 (ET-1) and endothelial nitric oxide (NO) synthase (eNOS) in normal and preeclamptic (PE) placentae. Methods The present cross-sectional analytical study was performed in normal and PE primigravidae (n = 10 in each group) who were admitted to the North Okkalapa General and Teaching Hospital from February 2019 to February 2020. Serum samples were collected immediately before delivery, and placental tissues were collected immediately after emergency or elective cesarean section. The expression of placental eNOS was measured by western blot, and the levels of ET-1 in placental tissue homogenates and in the serum were measured by enzyme-linked immunosorbent assay (ELISA). Results The PE group had significantly higher serum levels of ET-1 (median: 116.56 pg/mL; IQR: 89.14–159.62 pg/mL) than the normal group (median: 60.02 pg/mL; IQR: 50.89–94.37 pg/mL) (p < 0.05). However, statistically significant differences were not observed in the levels of ET-1 in placental tissue homogenates between normal and PE placentae (median: 0.007 pg/µg of total protein; IQR: 0.002–0.0123 pg/µg of total protein; and median: 0.005 pg/µg of total protein; IQR: 0.003–0.016 pg/µg of total protein respectively). The median and IQR values of relative placental eNOS expression were significantly higher in the PE group than in the normal group (p < 0.05). The serum levels of ET-1 level were not significantly correlated with placental ET-1 expression, and neither there was a significant correlation between placental ET-1 and eNOS expression in any of the groups. Conclusion The serum levels of ET-1 were significantly higher in PE pregnant women compared with normal pregnant women, while the ET-1 levels of placental tissue homogenates were not significantly different. Serum ET-1 rather than placental ET-1 might play a major role in the pathogenesis of PE.

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Section: Discussioncontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Expression of Endothelin-1 and Endothelial Nitric Oxide Synthase in Normal and Preeclamptic Placentae

Khaing

Swe

Aung

et al. 2022

Rev Bras Ginecol Obstet

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“…On the other hand, E-cadherin is a cell-cell adhesion protein that plays a crucial role in controlling the invasive phenotype of placenta accrete extravillous trophoblasts [31]. A previous study confirmed the high expression of E-cadherin in the preeclamptic placenta [32]. N-cadherin is also a key EMT marker involved in trophoblast invasion [33].…”

Section: Discussionmentioning

confidence: 91%

Involvement of WNT2 in trophoblast cell behavior in preeclampsia development

Liu

Huang

et al. 2020

Cell Cycle

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This study aimed to determine the WNT2 expression in patients with severe preeclampsia and to explore the function of WNT2 dysregulation on the biological behaviors of trophoblast cells. The WNT2 and β-catenin expression in the patients with early-onset and late-onset severe preeclampsia and normal controls was determined. Subsequently, WNT2 was overexpressed and knocked down in HTR8 cells and WNT2 signaling pathway in regulating trophoblast cell proliferation, migration, invasion, and apoptosis were evaluated in vitro. The mRNA and protein expression levels of WNT2 and β-catenin were decreased in patients with preeclampsia, especially early-onset severe preeclampsia. Overexpression of WNT2 promoted trophoblast cell proliferation, migration, and invasion and inhibited apoptosis in vitro, whereas knockdown of WNT2 had opposite effects. The findings of this study reveal that WNT2 and β-catenin were decreased expressed in patients with preeclampsia. Decreased expression of WNT2 may inhibit trophoblast cell proliferation, migration, and invasion but induced apoptosis. WNT2 may serve as a promising biomarker for early detection of preeclampsia.

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“…Migration of CD8 + memory cells largely depends on the binding of P-and E-selectins, the expression of which increases in the presence of IL-15 [66]. CD8 + T lymphocytes are characterized by expression of the CXCR6 receptor, which, through interaction with the chemokine CXCL16 secreted by trophoblast, attracts T lymphocytes to the decidua [52]. CD103 expression can be associated with the migration of CD8 + Treg into the inflam mation zone [56,84].…”

Section: Differentiation Of Cd8 + T Lymphocytesmentioning

confidence: 99%

“…Currently, there is no consensus on the persistence of the population of cytotoxic lymphocytes in the decidua during pregnancy. Some authors claim that during the pregnancy, the amount of CD8 + T lymphocytes in the decidua of women does not change [145] and is 45-75% of the T lymphocytes of the decidua (T lymphocytes constitute 10-20% of decidual leukocytes) [34,52,93]. Other authors point out a different content of cytotoxic lymphocytes in the decidua, depending on the period of pregnancy.…”

Section: Differentiation Of Cd8 + T Lymphocytesmentioning

confidence: 99%

The Role of Subpopulations of Cd8+ T Lymphocytes in the Development of Pregnancy

Степанова¹,

Bazhenov²,

Khokhlova³

et al. 2018

Med. immunol.

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At the present time, a broad spectrum of CD8+ T lymphocyte subsets is revealed, including naïve cells, memory cells and regulatory subpopulations. Along with cells with high cytolytic activity, some subsets with marked regulatory activity were found there. Each subpopulation is characterized by a set of produced mediators, surface and intracellular markers allowing to suggest their differential in vivo functional activity. The present review article proposes a classification of CD8+ Т cells which takes into account their morphological and functional features. According to conventional view, the CD8+ Т lymphocytes is a cell population exhibiting high cytotoxic ability which is of critical significance in pregnancy, under the conditions of semi-allogenic fetal cell invasion into the endometrium. The fraction of CD8+ T cells is rather high in decidual structures. The review discusses the known mechanisms of differentiation regulation, selective migration and activity of CD8+ T cells in decidual membrane and placenta in the course of pregnancy. Perforine and granzyme are the main cytotoxicity factors of CD8+ Т cells. IL-2, IL-5, IL-13, IFNγ, IL-17, TGF-β and IL-10 cytokines are considered regulatory mediators of CD8+ cells. To induce the effector properties of CD8+ T cells, an antigenic stimulation is required, which is provided by interactions between the CD8+ Т cells and activated CD4+ Т cells or dendritic cells, cytokine effects. Specific differentiation of the CD8+ T cells is determined by differences in microenvironvent. In the course of pregnancy, accumulation of CD8+ Т cells is observed in decidual membrane, but their phenotype and functional properties differ from CD8+ Т cells in peripheral blood. At present time, the mechanisms of selective CD8+ T cell migration to decidual membrane are studied. These events are suggested to be mediated by means of CXCR3 and CCR5 chemokine receptors, IL-6 and IL-15 cytokines. The features of CD8+ Т cell activities, and production of some cytokines, e.g., CSF2, IFNγ, IL-1β, IL-2, IL-6, IL-8,IL-10, IL-12 and TNFα in decidual membrane and is of critical significance for effective invasion of trophoblast cells. In turn, the trophoblast and placental cells promote development of regulatory CD8+ Т lymphocytes in decidual membrane, being able to induce CD8+ T cell apoptosis in decidual membrane. Hence, interaction between the maternal CD8+ T cells and trophoblast in the area of uterine-placental contact is an important link during development of immunological tolerance in the maternal/fetal system.

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Increased Expression of E-cadherin, Endothelin-1, and CD68 in Preeclamptic Placentas

Cited by 5 publications

References 22 publications

Expression of Endothelin-1 and Endothelial Nitric Oxide Synthase in Normal and Preeclamptic Placentae

Expression of Endothelin-1 and Endothelial Nitric Oxide Synthase in Normal and Preeclamptic Placentae

Involvement of WNT2 in trophoblast cell behavior in preeclampsia development

The Role of Subpopulations of Cd8+ T Lymphocytes in the Development of Pregnancy

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