Increased expression of HSP70, HSP90 and P53 in tissue of patients with periodontal and gingivitis deseases (59.6)

Abstract

Periodontal disease (PD) is a degenerative and progressive inflammatory disease induced by supragingival plaque bacteria, which affects the tooth supporting tissue such as periodontal ligament, cementum and alveolar bone. The host response to infection by periodontal bacteria may be related to a process of acute humoral response or cellular response. The expression of heat shock proteins (Hsp) and P53 is important in apoptosis, cell cycle control in cellular homeostasis and stress are overexpressed. The baseline of this study was to determine the expression of Hsp70, Hsp90 and P53 in the tissue of patients with chronic periodontal disease and gingivitis. The oral tissues were obtained by surgery, and quantified the amount of protein and characterized by SDS-PAGE, Western blot. To detect the expression of Hsp70, Hsp90 and p53 were using monoclonal antibodies. Hsp 70 detecction by RT-PCR were performed using Super ScriptTM One-Step and densitometric analysis. Results: There was an increase of Hsp70 and 90, and a slight increase in p53 levels. By PCR Hsp70 expression was increased in initial and progresive periodontitis and in a low level in advanced.