Increased expression of IL-1 receptors in response to IL-1β may produce more IL-6, IL-8, VEGF, and PGE2 in senescent synovial cells induced in vitro than in presenescent cells

Park, Jina; Choi, Hyun Rim; Yang, Haesik; Yoo, Myung Chul; Kim, Kyoung Soo

doi:10.1007/s00296-011-1891-1

Cited by 9 publications

(5 citation statements)

References 14 publications

(16 reference statements)

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“…RA-derived FLS, which undergo replicative senescence induced by serial passage, produce significantly more IL-6, IL-8, vascular endothelial growth factor (VEGF) and prostaglandin E2 (PGE2) in response to IL-1β, when compared to early-passage FLS [202]. Analysis of the molecular mechanisms underlying SASP revealed a critical role of persistent DNA damage response involving increased levels of proteins from the ATM pathway (ATM, NBS1, CHK2) [203, 204].…”

Section: The Nine Hallmarks Of Aging and Ramentioning

confidence: 99%

Rheumatoid Arthritis, Immunosenescence and the Hallmarks of Aging

Chalan¹,

Berg²,

Kroesen

et al. 2015

CAS

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Age is the most important risk factor for the development of infectious diseases, cancer and chronic inflammatory diseases including rheumatoid arthritis (RA). The very act of living causes damage to cells. A network of molecular, cellular and physiological maintenance and repair systems creates a buffering capacity against these damages. Aging leads to progressive shrinkage of the buffering capacity and increases vulnerability. In order to better understand the complex mammalian aging processes, nine hallmarks of aging and their interrelatedness were recently put forward.RA is a chronic autoimmune disease affecting the joints. Although RA may develop at a young age, the incidence of RA increases with age. It has been suggested that RA may develop as a consequence of premature aging (immunosenescence) of the immune system. Alternatively, premature aging may be the consequence of the inflammatory state in RA. In an effort to answer this chicken and egg conundrum, we here outline and discuss the nine hallmarks of aging, their contribution to the pre-aged phenotype and the effects of treatment on the reversibility of immunosenescence in RA.

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Section: The Nine Hallmarks Of Aging and Ramentioning

confidence: 99%

Rheumatoid Arthritis, Immunosenescence and the Hallmarks of Aging

Chalan¹,

Berg²,

Kroesen

et al. 2015

CAS

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“…Interleukin‐1β (IL‐1β) is a cytokine released by activated macrophages and monocytes during the acute phase of the inflammatory response . IL‐1β is a potent stimulator of the HPA axis by releasing CRH, adrenocorticotropic hormone (ACTH), and glucocorticoids . Although there is strong evidence supporting the influence of altered neuroendocrine and immune responses in mood disorders, the neurobiological factors involved in suicidal behavior have been scarcely researched.…”

mentioning

confidence: 99%

Immune dysfunction in bipolar disorder and suicide risk: is there an association between peripheral corticotropin‐releasing hormone and interleukin‐1β?

et al. 2014

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“…TNF-α may directly promote osteoclast maturation and differentiation by inducing the expression of macrophage colony-stimulating factor and receptor activator of nuclear factor κB ligand (RANKL) in osteoblasts (50). Additionally, TNF-α may directly promote RANKL-exposed precursor cells to differentiate into osteoclasts, activate mature osteoclasts and inhibit osteoclast apoptosis (51). According to the present results, CGRP pretreatment inhibited PgLPS-induced osteoblast apoptosis; however, this phenomenon was reversed by the addition of TNF-α.…”

Section: Discussionmentioning

confidence: 99%

Calcitonin gene‑related peptide reduces Porphyromonasï¿½gingivalis LPS‑induced TNF‑α release and apoptosis in osteoblasts

Zhou

Zhang

et al. 2017

Mol Med Report

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Periodontal diseases comprise mixed bacterial infections mainly caused by Gram‑negative anaerobic bacteria. Lipopolysaccharides (LPS) are important virulence factors and periodontal pathogens, which change local cytokine levels and promote osteoblast apoptosis, thereby leading to an imbalance in bone remodeling mechanisms and accelerating bone loss. Calcitonin gene‑related peptide (CGRP) is a vasoactive neuropeptide that is released from sensory nerves and has a positive effect on osteoblast proliferation and differentiation. In addition, this small molecule peptide is an important immune regulator in the inflammatory response. The aim of the present study was to assess the in vitro effects of CGRP on Porphyromonas gingivalis (Pg)LPS‑induced osteoblast apoptosis. Osteoblast cultures were stimulated either with various concentrations of PgLPS (0, 25, 50, 100, 500 and 1,000 ng/ml) for 48 h or with 500 ng/ml PgLPS for various lengths of time (0, 6, 12, 24, 48 and 72 h). The PgLPS‑stimulated cells were pretreated with different concentrations of CGRP (0, 1, 10, 100 and 1,000 nM) and cell viability and apoptotic rates were measured by Cell Counting kit‑8 assays and flow cytometry, respectively. CGRP, cleaved (c)‑Caspase‑8 and c‑Caspase‑3 protein expression levels were analyzed by western blotting. Changes in cytokine expression levels, which included tumor necrosis factor (TNF)‑α, interleukin (IL)‑1β, IL‑6, monocyte chemotactic protein (MCP)‑1 and MCP‑2, were measured by ELISA. PgLPS was demonstrated to inhibit osteoblast viability and promote apoptosis in a time‑ and concentration‑dependent manner. CGRP expression was revealed to reduce PgLPS‑induced cytostatic activity and apoptosis in osteoblasts. CGRP also suppressed the PgLPS‑induced release of TNF‑α and inhibited the activation of c‑Caspase‑3 and c‑Caspase‑8, thus preventing apoptosis in osteoblasts. CGRP may be an important neuropeptide in bone remodeling and may reduce osteoblast apoptosis in inflammatory conditions. These results may provide a solid foundation for CGRP to serve as a new target for the treatment of periodontitis.

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Increased expression of IL-1 receptors in response to IL-1β may produce more IL-6, IL-8, VEGF, and PGE2 in senescent synovial cells induced in vitro than in presenescent cells

Abstract: Primary synovial cells with high passage number demonstrate increased production of proinflam-

Cited by 9 publications

References 14 publications

Rheumatoid Arthritis, Immunosenescence and the Hallmarks of Aging

Rheumatoid Arthritis, Immunosenescence and the Hallmarks of Aging

Immune dysfunction in bipolar disorder and suicide risk: is there an association between peripheral corticotropin‐releasing hormone and interleukin‐1β?

Calcitonin gene‑related peptide reduces Porphyromonasï¿½gingivalis LPS‑induced TNF‑α release and apoptosis in osteoblasts

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