Increased Expression of Metalloproteinase-8 and -13 on Articular Cartilage in a Rat Immobilized Knee Model

Andõ, Akira; Hagiwara, Yoshihiro; Tsuchiya, Masahiro; Onoda, Yoshito; Suda, Hiroshi; Chimoto, E.; Itoi, Eiji

doi:10.1620/tjem.217.271

Cited by 28 publications

(21 citation statements)

References 31 publications

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“…All samples were rapidly frozen and stored at −80 °C until being cut on cryostat. Serial sections were then cryosectioned at 20 μm using a cryostat which were obtained at the medial midcondylar region in sagittal plane [38,39]. …”

Section: Methodsmentioning

confidence: 99%

P2X3 and P2X2/3 Receptors Play a Crucial Role in Articular Hyperalgesia Development Through Inflammatory Mechanisms in the Knee Joint Experimental Synovitis

et al. 2016

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Osteoarthritis (OA) is a degenerative and progressive disease, characterized by cartilage breakdown and by synovial membrane inflammation, which results in disability, joint swelling and pain. The purinergic P2X3 and P2X2/3 receptors contribute to development of inflammatory hyperalgesia, participate in arthritis processes in the knee joint and are expressed in chondrocytes and nociceptive afferent fibers innervating the knee joint. In this study, we hypothesized that P2X3 and P2X2/3 receptors activation by endogenous ATP (adenosine 5′-triphosphate) induces articular hyperalgesia in the knee joint of male and female rats through an indirect sensitization of primary afferent nociceptors dependent on the previous release of pro-inflammatory cytokines and/or on neutrophil migration. We found that the blockade of articular P2X3 and P2X2/3 receptors significantly attenuated carrageenan-induced hyperalgesia in the knee joint of male and estrus female rats in a similar manner. The carrageenan-induced knee joint inflammation increased the expression of P2X3 receptors in chondrocytes of articular cartilage. Further, the blockade of articular P2X3 and P2X2/3 receptors significantly reduced the increased concentration of TNF-α, IL-6 and CINC-1 and the neutrophil migration induced by carrageenan. These findings indicate that P2X3 and P2X2/3 receptors activation by endogenous ATP is essential to hyperalgesia development in the knee joint through an indirect sensitization of primary afferent nociceptors dependent on the previous release of pro-inflammatory cytokines and/or on neutrophil migration.

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Section: Methodsmentioning

confidence: 99%

P2X3 and P2X2/3 Receptors Play a Crucial Role in Articular Hyperalgesia Development Through Inflammatory Mechanisms in the Knee Joint Experimental Synovitis

et al. 2016

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“…The protocol used in the present study has been reported elsewhere 31–33 and is only briefly described as follows. The sections were deparaffinized and washed in PBS, pH 7.4, and then emersed in 0.2 N HCl for 20 min.…”

Section: Methodsmentioning

confidence: 99%

“…The total RNA of the homogenate was purified using an RNeasy lipid tissue mini kit (Qiagen). Complementary DNA was synthesized using a Cloned AMV first‐strand cDNA synthesis kit (Invitrogen) 32, 33…”

Section: Methodsmentioning

confidence: 99%

“…PCR efficiencies and relative expression levels of collagen types I and III as a function of EF1α1 were calculated as previously described 32–34. Primer sequences for expression analysis were Col1α1, upstream primer, 5′AGG GAA CAA CTG ATG GTG CTA CTG 3′, downstream primer, 5′GGA CTG CTG TGC CAA AAT AAG AGA 3′; Col3α1, upstream primer, 5′AGG GAA CAA CTG ATG GTG CTA CTG 3′, downstream primer, 5′GGA CTG CTG TGC CAA AAT AAG AGA 3′; EF1α1, upstream primer, 5′TGA TGC CCC AGG ACA CAG AGA CT 3′, downstream primer, 5′CCC AAC ACC AGC AGC AAC AAT C 3′.…”

Section: Methodsmentioning

confidence: 99%

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Expression patterns of collagen types I and III in the capsule of a rat knee contracture model

Hagiwara

Andõ

Onoda

et al. 2009

Journal Orthopaedic Research

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Our objective was to determine the changes in expression of collagen types I and III in the capsule of a rat knee contracture model. The unilateral knee joints of adult male rats were rigidly immobilized at 150 degrees of flexion using a rigid plastic plate and screws for 3 days, 1, 2, 4, 8, and 16 weeks (immobilized group). Sham-operated animals had holes drilled in the femur and tibia with screws inserted without a plate (control group). The expression patterns of collagen types I and III in the anterior and posterior capsule were evaluated by in situ hybridization (ISH), quantitative real-time polymerase chain reaction (qPCR), immunohistochemistry (IHC), and Western blotting (WB). Expressions of collagen types I and III were decreased after immobilization compared to the control group by ISH and qPCR. The expression was not changed after immobilization compared to the control group by IHC and WB. The expression of mRNA and protein levels of collagen types I and III were not increased after immobilization, which indicated that accumulation of the two types of collagen was not the etiology of joint contracture. Another process, such as capsule and synovial adhesions, may be one possible cause of joint contracture.

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“…), upregulation of destructive factors in the ECM (Ando et al. ; Leong et al. ), and cartilage surface alterations (Nagai et al.…”

Section: Introductionmentioning

confidence: 99%

Remobilization causes site‐specific cyst formation in immobilization‐induced knee cartilage degeneration in an immobilized rat model

et al. 2016

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An understanding of the articular cartilage degenerative process is necessary for the prevention and treatment of joint disease. The present study aimed to examine how long‐term immobilization‐induced cartilage degeneration is aggravated by remobilization. Sixty 8‐week‐old male Wistar rats were used in this study. The unilateral knee joint was immobilized using an external fixator for 8 weeks. The rats were killed at 0 and 3 days, and at 1, 2, 4 and 8 weeks after removing the fixator. After the rats were killed, the maximum knee extension angles were measured. Histological sections at the medial mid‐condylar region (non‐contact, transitional and contact regions of the femur and tibia) were prepared and scored. The cartilage thickness and number of chondrocytes were measured, and CD44 and Col2‐3/4c expression levels were assessed immunohistochemically. The histological assessment revealed progressive aggravation of cartilage degeneration in the transitional region, with a decreased number of chondrocytes and CD44‐positive chondrocytes as well as poor scoring over time, particularly in the tibia. Cyst formation was confirmed in the transitional region of the tibia at 8 weeks post‐remobilization. The cartilage thickness in the transitional region was thicker than that in the contact region, particularly in the tibia. Col2‐3/4c expression was observed in the non‐contact and transitional regions, and the knee extension angle was recovered. In conclusion, immobilization‐induced cartilage degeneration was aggravated by remobilization over time in the transitional region, followed by observations of a decreased number of chondrocytes and morphological disparity between different cartilage regions.

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Increased Expression of Metalloproteinase-8 and -13 on Articular Cartilage in a Rat Immobilized Knee Model

Cited by 28 publications

References 31 publications

P2X3 and P2X2/3 Receptors Play a Crucial Role in Articular Hyperalgesia Development Through Inflammatory Mechanisms in the Knee Joint Experimental Synovitis

P2X3 and P2X2/3 Receptors Play a Crucial Role in Articular Hyperalgesia Development Through Inflammatory Mechanisms in the Knee Joint Experimental Synovitis

Expression patterns of collagen types I and III in the capsule of a rat knee contracture model

Remobilization causes site‐specific cyst formation in immobilization‐induced knee cartilage degeneration in an immobilized rat model

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