Increased expression of phospholipase D1 in the spinal cords of rats with experimental autoimmune encephalomyelitis

Ahn, Meejung; Min, Do Sik; Kang, Jongchul; Jung, Kyungsook; Shin, Taekyun

doi:10.1016/s0304-3940(01)02383-7

Cited by 23 publications

(14 citation statements)

References 17 publications

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“…there have been several reports on localization of expression of PLD isoforms in the rodent CNS (Lee et al, 2000a,b;Saito et al, 2000;Ahn et al, 2001) that differ in part from the results we describe here. These discrepancies most likely ensue from the nature of the antibodies that were used previously coupled with a lack of confirmation of cell identity by double immunostaining.…”

Section: Differential Expression Of Pld1 and Pld2 In The Rodent Cnscontrasting

confidence: 91%

Increased expression of two phospholipase D isoforms during experimentally induced hippocampal mossy fiber outgrowth

Zhang

Huang

et al. 2004

Glia

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Mammalian phospholipase D (PLD), a multifunctional signaling enzyme, has been reported to facilitate neurite outgrowth in cultured neurons. However, two mammalian isoforms have been found, PLD1 and PLD2, and it has not been determined which isoform is involved, or whether this in vitro phenomenon is relevant to neurite extension in vivo. Using confocal microscopy, we demonstrate that the PLDs are expressed by different cell types in the mouse brain: PLD1 by neurons, and PLD2 by astrocytes. Moreover, using a model of experimentally induced hippocampal mossy fiber sprouting, both isoforms were observed to increase dramatically in expression level along tracts of mossy fiber spouting, supporting the proposal that PLD plays a role in this process. Given that the two isoforms undertake unique molecular functions in cultured cells, our findings suggest that in vivo PLD1 and PLD2 may modulate neuronal plasticity via different pathways and cell types.

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Section: Differential Expression Of Pld1 and Pld2 In The Rodent Cnscontrasting

confidence: 91%

Increased expression of two phospholipase D isoforms during experimentally induced hippocampal mossy fiber outgrowth

Zhang

Huang

et al. 2004

Glia

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“…Female Lewis rats, weighing 160–200 g and aged 8–12 weeks were used to actively induce EAE as described (Ahn et al . 2001).…”

Section: Experimental Autoimmune Encephalomyelitis (Eae) Inductionmentioning

confidence: 99%

“…Female Lewis rats, weighing 160-200 g and aged 8-12 weeks were used to actively induce EAE as described (Ahn et al 2001). Between thirteen and fourteen days after immunization, severe paralyzed rats were intracardiacally perfused, after which sections were prepared for immunofluorescent staining and electromicroscopic study.…”

Section: Experimental Autoimmune Encephalomyelitis (Eae) Inductionmentioning

confidence: 99%

Recombinant DNA vaccine encoding multiple domains related to inhibition of neurite outgrowth: a potential strategy for axonal regeneration

Nie

Chen

et al. 2004

Journal of Neurochemistry

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Myelin-derived proteins, such as tenascin-R (TN-R), myelin associate glycoprotein (MAG), and Nogo-A, inhibit the CNS regeneration. By targeting specifically the inhibitory epitopes, we have investigated whether vaccination with a recombinant DNA molecule encoding multiple domains of myelin inhibitors may be useful in CNS repair. We show here that the recombinant DNA vaccine is able to activate the immune system but does not induce experimental autoimmune encephalomyelitis (EAE) in Lewis rats.Importantly, it promotes axonal regeneration in a spinal cord injury model. Thus, the application of DNA vaccine, encoding multiple specific domains of major inhibitory proteins and/or their receptors, provides another promising approach to overcome the inhibitory barriers during CNS regeneration.

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“…[8] Several studies have shown that PLD1 is expressed in glial cells, such as presumed astrocytes in the rat central nervous system, [3] and that PLD1 is upregulated in astrocytes in response to transient forebrain ischemia [13] and in spinal cords in experimental autoimmune encephalomyelitis. [1] Little is known about the expression of PLD1 isozyme in peripheral nervous tissue. [7] Experimental autoimmune neuritis (EAN) is an autoimmune disease model of human peripheral demyelinating disease mediated by CD4þ T cells.…”

Section: Introductionmentioning

confidence: 99%

Increased Expression of Phospholipase D1 in the Sciatic Nerve of Rats With Experimental Autoimmune Neuritis

Shin

Min

Ahn

et al. 2002

Immunological Investigations

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Phospholipase D1 (PLD1) expression in the sciatic nerve was studied in induced experimental autoimmune neuritis (EAN) in Lewis rats. PLD1 immunoreactivity was seen in some Schwann cells in the sciatic nerves of normal rats. In parallel with the progression of EAN, PLD1-positive Schwann cells significantly increased in number and showed intense immunoreactivity. PLD1 was also detected in some ED1+ macrophages in EAN lesions. These results suggest that PLD1 in macrophages and Schwann cells plays an important role in the activation of these cells in the pathogenesis of EAN, an animal model of human peripheral demyelinating disease.

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Increased expression of phospholipase D1 in the spinal cords of rats with experimental autoimmune encephalomyelitis

Cited by 23 publications

References 17 publications

Increased expression of two phospholipase D isoforms during experimentally induced hippocampal mossy fiber outgrowth

Increased expression of two phospholipase D isoforms during experimentally induced hippocampal mossy fiber outgrowth

Recombinant DNA vaccine encoding multiple domains related to inhibition of neurite outgrowth: a potential strategy for axonal regeneration

Increased Expression of Phospholipase D1 in the Sciatic Nerve of Rats With Experimental Autoimmune Neuritis

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