Increased expression of stearoyl-CoA desaturase (SCD1) in the adipose tissue contributes to serum content of monounsaturated fatty acids (MUFA) in patients with chronic kidney disease

Abstract

The aim of the present study was to clarify whether the activity of SCD1 in adipose tissue contributes to the increased content of serum MUFA in patients with CKD. Moreover, gene expression of SREBP-1c in adipose tissue was assessed to get further insight into the mechanisms responsible for the observed alterations in serum MUFA profile in those patients. Methods Plasma samples were collected from 46 patients with CKD stage 5 (predialysis and dialyzed), as well as from 57 controls without CKD. Subcutaneous adipose tissue was taken from 22 of the patients with CKD at the time of kidney transplantation, and from 11 controls during hernia surgery. Serum and adipose tissue MUFA contents were assessed by gas chromatography-mass spectrometry as described previously. 3 SCD1 activity was estimated based on an oleic acid / stearic acid desaturation index (18:1/18:0 DI). SCD1 and SREBP-1c mRNA levels were measured in samples of subcutaneous adipose tissue of patients and controls by real-time reverse transcriptase-polymerase chain reaction. Dietary habits were assessed with the use of the Food Frequency Questionnaire with 6 answers (FFQ6). 5 FFQ6 is the most common dietary assessment tool used in epidemiologic studies, validated for Polish population. It consists of a list of 55 categories, further divided into specific foods or beverages. The major PUFA-rich products assessed included: oils, nuts, seeds, and various fish. The data are presented as mean and SD or median and interquartile range (IQR), as appropriate. The assumption

Increased adiponectin gene expression in adipose tissue may be related to an abnormal serum fatty acid profile in patients with chronic kidney disease

“…A research scheme showing the recruitment of study subjects and collection of biological material from them is presented as a supplementary Figure S1. The characteristics of these patients had been reported previously [2,8]. Blood samples were taken after overnight fasting from all the study subjects.…”

“…FA profiles were analyzed by gas chromatography-mass spectrometry (GC-MS) using QP-2010SE apparatus (Shimadzu, Kyoto, Japan), as described previously [2,8].…”

The Impact of Kidney Transplantation on the Serum Fatty Acid Profile in Patients with End-Stage Kidney Disease

“…CKD promotes FA disorders by metabolic alterations and dietary restrictions resulting from chronic intoxication. Previous research has shown changes in the proportion of individual serum FAs in CKD patients; in particular, the content of serum monounsaturated FAs (MUFAs) is increased [6], and the content of n-3 polyunsaturated FAs (PUFAs) is decreased [7][8][9][10][11].…”