“…Cells were supplemented with one of the following FA for 48h: palmitic acid (PAL, 16:0), oleic acid (OA, 18:1), docosahexaenoic acid (DHA, 22:6 n-3) and arachidonic acid (AA, 20:4 n-6) in three different concentrations (25µM, 50µM, and 100µM). Moreover, 3T3L1 adipocytes were supplemented with FA mix pooled from the FA isolated from serum of CKD stage 5 patients and healthy controls -from randomly selected patients and control serum samples, total lipids were extracted and hydrolyzed as we described previously [2,5].…”