Increased food intake after starvation enhances sleep in Drosophila melanogaster

Regalado, Josue M.; Cortez, McKenna B.; Grubbs, Jeremy J.; Link, Jared A.; Linden, Alexander M. van der; Zhang, Yong

doi:10.1016/j.jgg.2017.05.006

Cited by 23 publications

(22 citation statements)

References 31 publications

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“…A period of starvation in flies increases their consumption of liquid food media 10 , 11 , 39 , 40 . To determine whether starvation increases consumption of solid media, we compared 4 h consumption-excretion in fully fed and starved flies.…”

Section: Resultsmentioning

confidence: 99%

“…The capillary feeding (CAFE) assay has been used extensively to measure consumption of liquid diets in flies 10 , 11 , 31 , 39 , 41 . We coupled CAFE and Con-Ex methods in a single experimental design to address whether the volume of Blue 1 excreted might reflect the amount of Blue 1 consumed.…”

Section: Resultsmentioning

confidence: 99%

“…The fruit fly ( Drosophila melanogaster ) has emerged as a powerful model for investigating the effects of diet on both physiological and disease-like states. Studies in flies have revealed that diet has substantial effects on lifespan 1 – 5 , egg-laying 6 , metabolism 7 , fat deposition 8 , perceived nutritional value of food 9 , food choice 10 , sleep 11 and a host of other phenotypes 4 , 5 , 12 – 29 . Flies are housed on solid, agar-based media for days at a time in most laboratory studies.…”

Section: Introductionmentioning

confidence: 99%

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Measurement of solid food intake in Drosophila via consumption-excretion of a dye tracer

Shell

Schmitt

Lee

et al. 2018

Sci Rep

119

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Although the Drosophila melanogaster (fly) model is a popular platform for investigating diet-related phenomena, it can be challenging to measure the volume of agar-based food media flies consume. We addressed this challenge by developing a dye-based method called Consumption-Excretion (Con-Ex). In Con-Ex studies, flies consume solid food labeled with dye, and the volume of food consumed is reflected by the sum of the dye inside of and excreted by flies. Flies consumed-excreted measurable amounts of FD&C Blue No. 1 (Blue 1) and other dyes in Con-Ex studies, but only Blue 1 was readily detectable at concentrations that had no discernable effect on consumption-excretion. In studies with Blue 1, consumption-excretion (i) increased linearly with feeding duration out to 24 h at two different laboratory sites, (ii) was sensitive to starvation, mating status and strain, and (iii) changed in response to alteration of media composition as expected. Additionally, the volume of liquid Blue 1 consumed from capillary tubes was indistinguishable from the volume of Blue 1 excreted by flies, indicating that excreted Blue 1 reflects consumed Blue 1. Our results demonstrate that Con-Ex with Blue 1 as a food tracer is a useful method for assessing ingestion of agar-based food media in adult flies.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Measurement of solid food intake in Drosophila via consumption-excretion of a dye tracer

Shell

Schmitt

Lee

et al. 2018

Sci Rep

119

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“…Fruit flies are an important genetic model organism for investigating the molecular basis of a plethora of physiological outputs including alcohol‐related behaviors, food consumption, and responses to diet . To the best of our knowledge, our studies are the first to integrate these three areas of biology in the fly.…”

Section: Discussionmentioning

confidence: 99%

Dietary yeast influences ethanol sedation in Drosophila via serotonergic neuron function

et al. 2019

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Abuse of alcohol is a major clinical problem with far‐reaching health consequences. Understanding the environmental and genetic factors that contribute to alcohol‐related behaviors is a potential gateway for developing novel therapeutic approaches for patients that abuse the drug. To this end, we have used Drosophila melanogaster as a model to investigate the effect of diet, an environmental factor, on ethanol sedation. Providing flies with diets high in yeast, a routinely used component of fly media, increased their resistance to ethanol sedation. The yeast‐induced resistance to ethanol sedation occurred in several different genetic backgrounds, was observed in males and females, was elicited by yeast from different sources, was readily reversible, and was associated with increased nutrient intake as well as decreased internal ethanol levels. Inhibition of serotonergic neuron function using multiple independent genetic manipulations blocked the effect of yeast supplementation on ethanol sedation, nutrient intake, and internal ethanol levels. Our results demonstrate that yeast is a critical dietary component that influences ethanol sedation in flies and that serotonergic signaling is required for the effect of dietary yeast on nutrient intake, ethanol uptake/elimination, and ethanol sedation. Our studies establish the fly as a model for diet‐induced changes in ethanol sedation and raise the possibility that serotonin might mediate the effect of diet on alcohol‐related behavior in other species.

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“…AKH signalling in turn is required for the carbohydrate-dependent release of DILP3 [74], and represses expression of DILP2,3 and 5 [71]. Moreover, feeding and diet as well as gustatory perception also influence activity and sleep [75][76][77], and AKHR is expressed by gustatory neurons including those that express the sugar-sensing receptor Gr5a [16]. AKH production or release is also modulated by diurnal myokine (unpaired 2)-secretion from muscles [78].…”

Section: Discussionmentioning

confidence: 99%

Endocrine fine-tuning of daily locomotor activity patterns under non-starving conditions in Drosophila

Pauls

Selcho

Raederscheidt

et al. 2020

Preprint

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body ATP-sensitive potassium channels [6]. This triggers AKH release in a calcium-and AMP-activated protein kinase-dependent fashion [13]. Similar to glucagon, AKH acts on energy stores in the fat body by mobilising carbohydrates in form of the haemolymph sugar trehalose (hypertrehalosaemic effect), and by mobilising lipid depots (adipokinetic effect) [8,[14][15][16].OA, an analogue to vertebrate noradrenaline, is considered to act as an insect stress or arousal hormone [17]. OA is produced by octopaminergic neurons (OANs) located mostly along the midline in the brain and ventral nerve cord [18]. Among other functions, OA exerts myotropic effects on skeletal muscles [19] and mediates the adaptation of muscle metabolism to the physiological demands of locomotion [20][21][22].Both AKH and OA are crucial for starvation-induced hyperactivity, as this behaviour is absent in flies with impaired AKH or OA signalling [4,5,[7][8][9]. AKH activates a subset of OANs which are required for starvation-induced hyperactivity [5,9]. These neurons express receptors for AKH and Drosophila insulin-like peptides (DILPs). DILPs act antagonistically to AKH, and suppress starvation-induced hyperactivity via the OANs [9]. Yu and colleagues (2016) showed that the AKH-dependent increase in locomotor activity upon starvation represents a neuromodulatory and behavioural role of AKH and is not an indirect consequence of altered energy metabolism.Remarkably, studies in various insect species suggest that AKH and also OA play a role in the regulation of locomotor activity when the insects have free access to food (reviewed in [23]). For example, the circulating haemolymph titres of AKH correlate positively with daily rhythms in walking activity in the linden bug Pyrrhocoris apterus [24,25]. In Pyrrhocoris [26,27], crickets [28] and the cockroach Periplaneta americana [29,30], injection into the haemolymph or topical application of AKH induces a strong increase in locomotor activity in ad libitum fed animals, independent of the time of day [27,29]. Also in Drosophila, genetic overexpression of AKH enhances locomotor activity when food is freely available [31]. Unlike for starvation-induced hyperactivity, however, it is unclear whether and how AKH:OAN signalling contributes to shape daily activity under ad libitum feeding conditions, or whether increased AKH signalling is a consequence of a locomotor-induced increase in energy demands. Cockroach AKHs are known to increase the spontaneous neuronal activity of octopaminergic dorsal unpaired median neurons in the central nervous system, which express a

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Increased food intake after starvation enhances sleep in Drosophila melanogaster

Cited by 23 publications

References 31 publications

Measurement of solid food intake in Drosophila via consumption-excretion of a dye tracer

Measurement of solid food intake in Drosophila via consumption-excretion of a dye tracer

Dietary yeast influences ethanol sedation in Drosophila via serotonergic neuron function

Endocrine fine-tuning of daily locomotor activity patterns under non-starving conditions in Drosophila

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