Increased frequency of <scp>CD45</scp> negative T cells (T zone cells) in older Golden retriever dogs

Hughes, Ken; Labadie, Julia D.; Yoshimoto, Janna; Dossey, Jeremy; Burnett, Robert; Avery, Anne C.

doi:10.1111/vco.12343

Cited by 15 publications

(12 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The PCR products in the 420 range (black) were amplified with V gamma 3 primer. 8 Approximately 10 d after aspirates were acquired, biopsies of the cranial mediastinal mass, liver, gastrointestinal tract, mesenteric and prescapular lymph nodes, and spleen were obtained for evaluation and fixed in 10% neutral-buffered formalin. Tissues were routinely processed and paraffin-embedded, and tissue sections of 5-μm thickness were cut and stained with hematoxylin and eosin.…”

mentioning

confidence: 99%

Metastatic thymoma in the liver of a dog

et al. 2018

View full text Add to dashboard Cite

A 12-y-old neutered male Portuguese Water dog was presented because of a 1-y history of persistent hyporexia, diarrhea, and recurrent pyelonephritis. Abdominal ultrasound revealed hepatic nodules and diffuse splenomegaly, and radiographs revealed a mediastinal mass. Fine-needle aspirates of the liver, spleen, and mediastinal mass were suspicious for lymphoma. Flow cytometry identified small T cells that co-expressed CD4 and CD8 at all sites, most suspicious for thymoma, but lymphoma could not be ruled out. PCR for antigen receptor rearrangements analysis identified polyclonal amplification of the T-cell receptor genes, more consistent with thymoma than lymphoma. Histopathology of the liver and thymic mass confirmed thymoma with hepatic metastasis.

show abstract

mentioning

confidence: 99%

Metastatic thymoma in the liver of a dog

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Clinicians should combine data from the physical examination, clinical course, and biological behavior of the tumor, as well as results of flow cytometry and histopathology before making a final diagnosis of TZL. A recent study also suggested that PARR could be more sensitive than flow cytometry when the clonal cells still account for a minority of the lymphocyte population . In this study, a clonal T‐cell receptor gamma locus rearrangement was found in 34% of “healthy” Golden Retrievers with circulating T‐zone cells, and no clinical evidence of lymphoma or another lymphoproliferative disease .…”

Section: Discussionmentioning

confidence: 48%

“…A recent study also suggested that PARR could be more sensitive than flow cytometry when the clonal cells still account for a minority of the lymphocyte population . In this study, a clonal T‐cell receptor gamma locus rearrangement was found in 34% of “healthy” Golden Retrievers with circulating T‐zone cells, and no clinical evidence of lymphoma or another lymphoproliferative disease . Since the Golden Retriever breed is predisposed to develop TZL, Hughes et al.…”

Section: Discussionmentioning

confidence: 52%

See 1 more Smart Citation

A case of canine high‐grade T‐cell lymphoma immunophenotypically consistent with T‐zone lymphoma

Parachini‐Winter

Curran

Russell

et al. 2018

Veterinary Clinical Pathol

View full text Add to dashboard Cite

A 9-year-old spayed female Curly Coated Retriever was referred for evaluation of generalized peripheral lymphadenomegaly. The dog was clinically healthy on presentation with no anomalies detected on complete blood count, serum biochemistry, urinalysis, or three-view thoracic radiographs. Fine-needle aspiration (FNA) and cytology of the peripheral lymph nodes were consistent with lymphoma with an intermediate-sized lymphoid population. Flow cytometry of peripheral lymph nodes was consistent with a homogeneous population of CD4 T cells that had lost expression of the pan-leukocyte antigen CD45. Variable expression of CD21, CD25, and class II major histocompatibility complex (MHC) were also noted. This was considered consistent with T-zone lymphoma (TZL), although the T cells were noted to be larger than usual based on flow cytometry. Due to the suspected indolent nature of this patient's disease and clinical progression, a careful monitoring approach was initially discussed with the owner. However, additional diagnostic testing was performed to confirm the diagnosis. Bone marrow cytology did not show any significant anomalies. The largest lymph node (left mandibular) was extirpated and submitted for histopathology. Based on the lymph node architecture, cellular features, and high mitotic activity, an unexpected diagnosis of peripheral T-cell lymphoma not otherwise specified (PTCL-NOS) was made. The dog was started on CHOP (cyclophosphamide, doxorubicin, vincristine, and prednisone) chemotherapy. This case illustrates the limitations of using flow cytometry as the sole means of diagnosing TZL and highlights the importance of using complementary tests when subtyping canine lymphoma, which is significant when considering a patient's treatment plan and prognosis.

show abstract

“…4 Hughes et al also enlightened that the immunophenotype of TZL is unique in that "the neoplastic T cells do not express the pan-leukocyte antigen CD45, allowing for a reliable diagnosis by immunophenotyping." 8 Our goal with this case report was to emphasize the necessity to dig deeper and combine diagnostics such as flow cytometry and histopathology to reach an accurate diagnosis in puzzling cases. We acknowledge that a lymph node biopsy can sometimes be both a technical and financial hurdle, and this procedure is not offered as part of our standard workup for more straightforward lymphoma patients.…”

mentioning

confidence: 99%