Increased frequency of X-bearing sperm in males from an infertility clinic: analysis by  two-color fluorescence in situ hybridization

Johannisson, R.; Leuschner, Elke; Hüppe, M; Hinrichs, Frauke; Al-Hasani, S.; Diedrich, K.; Schwinger, E.; Mennicke, Kirsten

doi:10.1159/000071041

Cited by 18 publications

(11 citation statements)

References 28 publications

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“…Image processing and scoring were performed using custom software developed in MATLAB (Mathworks Inc., Natick, MA), using classification and scoring algorithms based on the scoring criteria described by Baumgartner et al (1999). The overall hybridization efficiency was 97%, which is consistent with the hybridization efficiency reported by other groups (Johannisson et al 2002; Martin et al 1996; Tiido et al 2005). These methods have been described in detail elsewhere (Perry et al 2011).…”

Section: Methodssupporting

confidence: 77%

Environmental Exposure to Polychlorinated Biphenyls and p,p ´-DDE and Sperm Sex-Chromosome Disomy

McAuliffe

Williams

Korrick

et al. 2012

Environ Health Perspect

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Background: Chromosomal abnormalities contribute substantially to reproductive problems, but the role of environmental risk factors has received little attention.Objectives: We evaluated the association of polychlorinated biphenyl (PCB) and dichlorodiphenyldichloroethylene (p,p´-DDE) exposures with sperm sex-chromosome disomy.Methods: We conducted a cross-sectional study of 192 men from subfertile couples. We used multiprobe fluorescence in situ hybridization (FISH) for chromosomes X, Y, and 18 to determine XX, YY, XY, and total sex-chromosome disomy in sperm nuclei. Serum was analyzed for concentrations of 57 PCB congeners and p,p´-DDE. Poisson regression models were used to calculate incidence rate ratios (IRRs) for disomy by exposure quartiles, controlling for demographic characteristics and semen parameters.Results: The median percent disomy was 0.3 for XX and YY, 0.9 for XY, and 1.6 for total sex-chromosome disomy. We observed a significant trend of increasing IRRs for increasing quartiles of p,p´-DDE in XX, XY, and total sex-chromosome disomy, and a significant trend of increasing IRRs for increasing quartiles of PCBs for XY and total sex-chromosome disomy; however, there was a significant inverse association for XX disomy.Conclusions: Our findings suggest that exposure to p,p´-DDE may be associated with increased rates of XX, XY, and total sex-chromosome disomy, whereas exposure to PCBs may be associated with increased rates of YY, XY, and total sex-chromosome disomy. In addition, we observed an inverse association between increased exposure to PCBs and XX disomy. Further work is needed to confirm these findings.

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Section: Methodssupporting

confidence: 77%

Environmental Exposure to Polychlorinated Biphenyls and p,p ´-DDE and Sperm Sex-Chromosome Disomy

McAuliffe

Williams

Korrick

et al. 2012

Environ Health Perspect

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“…Also the overall hybridization efficiency was from 98.91 to 99.36% which is higher with the hybridization efficiency (97%) reported by other groups (Johannisson et al, 2002;Tiido et al, 2005;Martin, 2006).…”

Section: Tablecontrasting

confidence: 65%

The association between environmental exposure to pyrethroids and sperm aneuploidy

Radwan¹,

Jurewicz

Wielgomas

et al. 2015

Chemosphere

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“…However, a hybridization efficiency of 95% or more is in good accordance with the hybridization efficiency previously reported [67,68].…”

Section: Discussionsupporting

confidence: 91%

Serum levels of perfluorinated compounds and sperm Y:X chromosome ratio in two European populations and in Inuit from Greenland

Kvist

Giwercman

Jönsson

et al. 2012

Reproductive Toxicology

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This study investigated whether perfluorooctanoic acid (PFOA) and perfluorooctanesulfonate (PFOS), which exhibit reproductive toxicity in experimental animals, affect sperm sex chromosome ratio.The Y:X ratio was determined by fluorescence in situ hybridization. Serum concentrations of PFOA and PFOS were measured in 607 men from Greenland, Poland and Ukraine using liquid chromatography-tandem mass spectrometry. Data was analyzed by linear and nonlinear regression.We observed no associations between PFOA and Y:X ratio (p=0.845 in a linear model, p=0.296 in a nonlinear model). A positive nonlinear association between PFOS and Y:X ratio was observed (p=0.016), with no association in a linear model (p=0.118). Analyzing the populations separately, a negative trend between categorized PFOS exposure and Y:X ratio was observed for the Inuit (B=-0.002, p=0.044).In conclusion, there was a negative trend between Y:X ratio and PFOS in the Inuit, while there was no association between PFOA and the Y:X ratio in adult men.

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Increased frequency of X-bearing sperm in males from an infertility clinic: analysis by two-color fluorescence in situ hybridization

Cited by 18 publications

References 28 publications

Environmental Exposure to Polychlorinated Biphenyls and p,p ´-DDE and Sperm Sex-Chromosome Disomy

Environmental Exposure to Polychlorinated Biphenyls and p,p ´-DDE and Sperm Sex-Chromosome Disomy

The association between environmental exposure to pyrethroids and sperm aneuploidy

Serum levels of perfluorinated compounds and sperm Y:X chromosome ratio in two European populations and in Inuit from Greenland

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