Increased glycogen storage in yeast results in less branched glycogen

Wilson, Wayne A.; Hughes, William E.; Tomamichel, Wendy J.; Roach, Peter J.

doi:10.1016/j.bbrc.2004.05.180

Cited by 21 publications

(13 citation statements)

References 43 publications

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“…This could be ascribed to the fact that glycogen granule architecture is the result of the highly orchestrated actions of GlgB and other glycogen enzymes, which may collapse under GlgB overproduction conditions. 44 …”

Section: Resultsmentioning

confidence: 99%

Genome-Wide Screening of Genes Whose Enhanced Expression Affects Glycogen Accumulation in Escherichia coli

et al. 2010

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Using a systematic and comprehensive gene expression library (the ASKA library), we have carried out a genome-wide screening of the genes whose increased plasmid-directed expression affected glycogen metabolism in Escherichia coli. Of the 4123 clones of the collection, 28 displayed a glycogen-excess phenotype, whereas 58 displayed a glycogen-deficient phenotype. The genes whose enhanced expression affected glycogen accumulation were classified into various functional categories including carbon sensing, transport and metabolism, general stress and stringent responses, factors determining intercellular communication, aggregative and social behaviour, nitrogen metabolism and energy status. Noteworthy, one-third of them were genes about which little or nothing is known. We propose an integrated metabolic model wherein E. coli glycogen metabolism is highly interconnected with a wide variety of cellular processes and is tightly adjusted to the nutritional and energetic status of the cell. Furthermore, we provide clues about possible biological roles of genes of still unknown functions.

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Section: Resultsmentioning

confidence: 99%

Genome-Wide Screening of Genes Whose Enhanced Expression Affects Glycogen Accumulation in Escherichia coli

et al. 2010

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“…; Wilson et al . ). In glycogen accumulation and usage inhibition assays, normal growth of cells was affected in the presence of vulpinic acid because of its inhibitory effect on glycogen metabolism (Fig.…”

Section: Discussionmentioning

confidence: 97%

A chemogenomic approach to understand the antifungal action of Lichen-derived vulpinic acid

et al. 2016

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“…As with glycogen synthase, transcription of GLC3 is induced at the approach to the stationary phase, concomitant with the onset of glycogen storage (Rowen et al, 1992;DeRisi et al, 1997). There is no evidence for post-translational control of Glc3p and a key regulator of glycogen branching appears to be the balance between the activity of glycogen synthase and that of the branching enzyme (Raben et al, 2001;Pederson et al, 2003;Wilson et al, 2004).…”

Section: Branching Enzymementioning

confidence: 99%

Regulation of glycogen metabolism in yeast and bacteria

et al. 2010

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Microorganisms have the capacity to utilize a variety of nutrients and adapt to continuously changing environmental conditions. Many microorganisms, including yeast and bacteria, accumulate carbon and energy reserves to cope with starvation conditions temporarily present in the environment. Glycogen biosynthesis is a main strategy for such metabolic storage and a variety of sensing and signaling mechanisms have evolved in evolutionarily distant species to guarantee the production of this homopolysaccharide. At the most fundamental level, the processes of glycogen synthesis and degradation in yeast and bacteria share certain broad similarities. However, the regulation of these processes is sometimes quite distinct, indicating that they have evolved separately to respond optimally to the habitat conditions of each species. This review aims to highlight the mechanisms, both at the transcriptional and post-transcriptional levels, which regulate glycogen metabolism in yeast and bacteria, focusing on selected areas where the greatest increase in knowledge has occurred during the last few years. In the yeast system, we focus particularly on the various signaling pathways that control the activity of the enzymes of glycogen storage. We also discuss our recent understanding of the important role played by the vacuole in glycogen metabolism. In the case of bacterial glycogen, especial emphasis is given to aspects related with genetic regulation of glycogen metabolism and its connection with other biological processes.

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Increased glycogen storage in yeast results in less branched glycogen

Cited by 21 publications

References 43 publications

Genome-Wide Screening of Genes Whose Enhanced Expression Affects Glycogen Accumulation in Escherichia coli

Genome-Wide Screening of Genes Whose Enhanced Expression Affects Glycogen Accumulation in Escherichia coli

A chemogenomic approach to understand the antifungal action of Lichen-derived vulpinic acid

Regulation of glycogen metabolism in yeast and bacteria

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