Increased grain protein and gluten contents of bread wheat caused by introgression of a T. timopheevii segment into chromosome 2A

Shchukina, L. V.; Симонов, А. В.; Demenkova, M. A.; Klykov, A. G.; Шаманин, Владимир; Pozherukova, Violetta; Лепехов, С. Б.; Chebatareva, M. V.; Petin, V. A.; Börner, Andreas; Пшеничникова, Т. А.

doi:10.1007/s10681-022-03121-w

Cited by 8 publications

(6 citation statements)

References 30 publications

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“…These lines were obtained for various cultivars of bread wheat at the previous cytogenetic stage of studying its polyploid genome. In this and our previous work (Shchukina et al 2022), we used this approach to verify the effects of introgressions carrying high protein and gluten genes from three different cereal species (T. timopheevii, T. durum and Ae. speltoides).…”

Section: Discussionmentioning

confidence: 99%

“…A good way to investigate the phenotypic effect of such a gene with unknown exact intrachromosomal localization and function is to transfer the chromosome with the introgression to another genotype. In this way, the effect of a gene that increases the protein and gluten content in grain, introgressed from T. timopheevii, was proven (Shchukina et al 2022). For this purpose, 2A chromosome with introgression was transferred into the genotype of the spring cultivar Saratovskaya 29 using the corresponding monosomic line.…”

Section: Introductionmentioning

confidence: 99%

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Variability of protein and gluten content in bread wheat lines with introgressions into chromosome 5B from related species

Shchukina,

Klykov,

Murugova

et al. 2024

Preprint

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Numerous samples with introgressions were obtained in bread wheat in result of distant hybridization. However, only a small number of them were used for improvement of modern cultivars for such an important traits as protein and gluten content in grain. The aim of this study is to investigate the phenotypic impact of introgressions transferred to 5B chromosome of bread wheat from the species T. durum and Ae. speltoides on these grain quality traits. To this end, two lines that carried introgressions of different size in 5B chromosome were developed on the genetic background of cv. Saratovskaya 29 (S29). One line had the introgression from Aegilops speltoides in the distal region of the long arm of the chromosome, while another line - the introgression from Triticum durum in the centromeric region. Transfer of introgressions from donors to the recipient was confirmed using microsatellite markers. The lines were studied in remote geographical areas and in greenhouse. They showed a significant increase in protein and gluten content across all environments by 1% and 4%, respectively, with the greatest effect under normal moisture conditions. The lines retained high rheological and mixing dough properties characteristic of S29. The conducted studies made it possible to verify loci QGlc.ipk-5B and QDsa.ipk-5B previously identified in ITMI mapping population, associated with gluten content and dough resistance to mixing. It was also possible to confirm the possible association of introgressions into 5B chromosome with a decrease in thousand kernel weight.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Variability of protein and gluten content in bread wheat lines with introgressions into chromosome 5B from related species

Shchukina,

Klykov,

Murugova

et al. 2024

Preprint

Self Cite

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“…Additionally, introgressions from the 3R chromosome has been shown to contribute positively to baking quality stability across various drought treatments ( 33 ). Major genes with positive effects on functional quality have been transferred to wheat from other species than rye, e.g., Aegilops sharonensis ( 82 ), T. timopheevii ( 84 ), and Aegilops triuncialis ( 104 ). Additionally, introgressions from Aegilops spp., Agropyron spp., Thinopyrum spp., and Leymus spp., contributing major genes or quantitative traits loci (QTL), have been found to contribute positive effects to the functional properties of wheat ( 38 , 105–108 ).…”

Section: Functional and Nutritional Aspects—rooms For Improvement By ...mentioning

confidence: 99%

Alien introgression to wheat for food security: functional and nutritional quality for novel products under climate change

Johansson,

Lan,

Olalekan

et al. 2024

Front. Nutr.

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Crop yield and quality has increased globally during recent decades due to plant breeding, resulting in improved food security. However, climate change and shifts in human dietary habits and preferences display novel pressure on crop production to deliver enough quantity and quality to secure food for future generations. This review paper describes the current state-of-the-art and presents innovative approaches related to alien introgressions into wheat, focusing on aspects related to quality, functional characteristics, nutritional attributes, and development of novel food products. The benefits and opportunities that the novel and traditional plant breeding methods contribute to using alien germplasm in plant breeding are also discussed. In principle, gene introgressions from rye have been the most widely utilized alien gene source for wheat. Furthermore, the incorporation of novel resistance genes toward diseases and pests have been the most transferred type of genes into the wheat genome. The incorporation of novel resistance genes toward diseases and pests into the wheat genome is important in breeding for increased food security. Alien introgressions to wheat from e.g. rye and Aegilops spp. have also contributed to improved nutritional and functional quality. Recent studies have shown that introgressions to wheat of genes from chromosome 3 in rye have an impact on both yield, nutritional and functional quality, and quality stability during drought treatment, another character of high importance for food security under climate change scenarios. Additionally, the introgression of alien genes into wheat has the potential to improve the nutritional profiles of future food products, by contributing higher minerals levels or lower levels of anti-nutritional compounds into e.g., plant-based products substituting animal-based food alternatives. To conclude, the present review paper highlights great opportunities and shows a few examples of how food security and functional-nutritional quality in traditional and novel wheat products can be improved by the use of genes from alien sources, such as rye and other relatives to wheat. Novel and upcoming plant breeding methods such as genome-wide association studies, gene editing, genomic selection and speed breeding, have the potential to complement traditional technologies to keep pace with climate change and consumer eating habits.

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“…For species from the secondary and tertiary gene pools, the genomic composition of which differs from the A, B, and D genomes of common wheat, the published data on their quality traits are scarce, and most of them are devoted mainly to investigating the allelic diversity of storage proteins and their possible impact on improving quality indicators [34][35][36]. Increased content of wet gluten and protein in the grain has been found in the hybrid lines containing alien chromatin from species of Timopheevi group (T. timopheevii, T. araraticum, genomes A t A t GG), Aegilops speltoides (genome S), wheatgrass Thynopyrum intermedium (genomes EEE st E st S t S t ) [24,[37][38][39][40], which makes it possible to assume that the introgression fragments carried over from wheat relatives may contain the genetic factors affecting the GC.…”

Section: Of 15mentioning

confidence: 99%

“…However, the data on the QTLs inherited from T. timopheevii that contributed to the quality traits are very limited. Only Shchukina et al [38], who studied an introgression line with translocation from T. timopheevii in chromosome 2A, showed that the line differed by increased GC from the original bread wheat variety. Thus, the results of the GWAS we performed suggest that the QTL on chromosome 2A is a new locus that originated from the T. timopheevii genome because its localization differs from the previously reported GC-associated genes/QTLs.…”

Section: Comparison Of Putative Qtl Localization With Known Datamentioning

confidence: 99%

Novel Genetic Loci from Triticum timopheevii Associated with Gluten Content Revealed by GWAS in Wheat Breeding Lines

Leonova,

Kiseleva,

Berezhnaya

et al. 2023

IJMS

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The content and quality of gluten in wheat grain is a distinctive characteristic that determines the final properties of wheat flour. In this study, a genome-wide association study (GWAS) was performed on a wheat panel consisting of bread wheat varieties and the introgression lines (ILs) obtained via hybridization with tetraploid wheat relatives. A total of 17 stable quantitative trait nucleotides (QTNs) located on chromosomes 1D, 2A, 2B, 3D, 5A, 6A, 7B, and 7D that explained up to 21% of the phenotypic variation were identified. Among them, the QTLs on chromosomes 2A and 7B were found to contain three and six linked SNP markers, respectively. Comparative analysis of wheat genotypes according to the composition of haplotypes for the three closely linked SNPs of chromosome 2A indicated that haplotype TT/AA/GG was characteristic of ten ILs containing introgressions from T. timopheevii. The gluten content in the plants with TT/AA/GG haplotype was significantly higher than in the varieties with haplotype GG/GG/AA. Having compared the newly obtained data with the previously reported quantitative trait loci (QTLs) we inferred that the locus on chromosome 2A inherited from T. timopheevii is potentially novel. The introgression lines containing the new locus can be used as sources of genetic factors to improve the quality traits of bread wheat.

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Increased grain protein and gluten contents of bread wheat caused by introgression of a T. timopheevii segment into chromosome 2A

Cited by 8 publications

References 30 publications

Variability of protein and gluten content in bread wheat lines with introgressions into chromosome 5B from related species

Variability of protein and gluten content in bread wheat lines with introgressions into chromosome 5B from related species

Alien introgression to wheat for food security: functional and nutritional quality for novel products under climate change

Novel Genetic Loci from Triticum timopheevii Associated with Gluten Content Revealed by GWAS in Wheat Breeding Lines

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