Increased homocysteine, homocysteine-thiolactone, protein homocysteinylation and oxidative stress in the circulation of patients with Eales’ disease

Bharathselvi, Muthuvel; Biswas, Jyothirmay; Selvi, Radhakrishnan; Coral, Karunakaran; Angayarkanni, Narayanasamy; Ramakrishnan, Sivaramakrishnan; Sulochana, K N

doi:10.1177/0004563213492146

Cited by 9 publications

(4 citation statements)

References 42 publications

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“…On individual examination of each of these, four were duplicate publications of the same data set (Nowak et al 2004;Seddon et al 2006b;Javadzadeh et al 2012;Manresa et al 2015b). After removal of duplicates, 31 records were screened; of these, nine were excluded, because four were cohort studies (Robman et al 2004;Christen et al 2009Christen et al , 2015Gopinath et al 2013), two were review articles (McCarty 2000;Wong et al 2015), one was a meta-analysis (Huang et al 2015), one was a cell culture study (Roybal et al 2005) and one was about Eales' disease (Bharathselvi et al 2013). Twenty-two full-text articles were assessed for eligibility; of these, 10 were excluded, because two were commentaries (Evans 2013;Kawada 2013), two provided no data on plasma tHcy concentrations (Parmeggiani et al 2007;Sa a et al 2014) and six did not report suitable or sufficient data for calculation (Heuberger et al 2002;Vine et al 2005;Rochtchina et al 2007;Wu et al 2007;Keles et al 2014;Manresa et al 2015a).…”

Section: Resultsmentioning

confidence: 99%

Homocysteine and risk of age‐related macular degeneration: a systematic review and meta‐analysis

Pinna

Zaccheddu

Boscia

et al. 2016

Acta Ophthalmologica

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There is still no agreement on total plasma homocysteine (tHcy) role in age-related macular degeneration (AMD), the leading cause of new blindness in industrialized countries. We performed a systematic review and meta-analysis of the published data on the correlation between tHcy and AMD. MEDLINE/PubMed and ISI Web of Sciences searches were performed according to MOOSE guidelines. Case-control studies were eligible for inclusion. Participants and controls were AMD patients and subjects without AMD. The main outcome measure was wet AMD. Homocysteine level was the main exposure variable. Data were pooled using a random-effects model. Twelve case-control studies were identified: 10 assessed wet AMD, four dry AMD, one early AMD, one late AMD, and one any AMD. As for wet AMD, there was a total of 453 cases and 514 controls. Mean tHcy was on average 1.1 μmol/l (95% confidence interval [CI] = 0.96-1.25) greater in wet AMD cases, but there was evidence of extreme between-study heterogeneity (p < 0.001, I = 91.8%). In a model homogenous for age, including six wet AMD studies (214 cases, 274 controls), mean tHcy was on average 0.58 μmol/l (95% CI = 0.35-0.73) greater in the case group, a not statistically significant result (p = 0.144) associated with moderate heterogeneity (I = 39.2%). Our meta-analysis indicates that there is some weak evidence that increased tHcy might be associated with wet AMD; however, this result should be interpreted cautiously, because of a marked between-study heterogeneity and the possible effect of publication bias. Future studies, preferably of cohort design, are necessary before any firm conclusions on the putative role of increased tHcy on AMD can be drawn.

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Section: Resultsmentioning

confidence: 99%

Homocysteine and risk of age‐related macular degeneration: a systematic review and meta‐analysis

Pinna

Zaccheddu

Boscia

et al. 2016

Acta Ophthalmologica

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“…Measurement of protein homocysteinylation was performed by the method as described by Bharathselvi et al 16. The plasma levels of protein-Cysteine bound Hcy and protein-Lysine bound Hcy were expressed in μM of Hcy.…”

Section: Methodsmentioning

confidence: 99%

Homocysteine & its metabolite homocysteine-thiolactone & deficiency of copper in patients with age related macular degeneration - A pilot study

Bharathselvi

Biswas

Raman

et al. 2016

Indian Journal of Medical Research

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Background & objectives:Age related macular degeneration (ARMD) is a leading cause of blindness, particularly in persons above 60 yr of age. Homocysteine is implicated in many ocular diseases including ARMD. This study was undertaken to assess the status and relationship between plasma homocysteine, homocysteine - thiolactone, homocysteinylated protein and copper levels in patients with ARMD.Methods:A total of 16 patients with ARMD and 16 age-matched controls were recruited for the study. Plasma glutathione, homocysteine, homocysteine - thiolactone and extent of homocysteine conjugation with proteins, copper and thiobarbituric acid reactive substances were measured.Results:Homocysteine levels were elevated with increase in homocysteine-thiolactone, thiobarbituric acid reactive substances and a decrease of glutathione. The levels of homocysteinylated protein were elevated in ARMD. The elevated homocysteine, homocysteine-thiolactone correlated with the decrease in copper level.Interpretation & conclusions:Elevated homocysteine and its metabolite homocysteine-thiolactone and decreased levels of copper may play an important role in the pathogenesis of ARMD.

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“…In a case-control study carried out in RVO patients, we found a significant association between hemorheologic alterations and the occurrence of RVO [11]; in addition, some articles have reported a beneficial role for hemodilution therapy [12,13]. The role of oxidative stress in RVO patients has been poorly explored [14,15], and its contribution to hemorheologic abnormalities is still unknown. The objective of the present study was to determine whether erythrocytes from RVO patients show signs of oxidative stress, and whether this condition is associated with an altered hemorheologic profile.…”

Section: Introductionmentioning

confidence: 99%

Erythrocyte oxidative stress is associated with cell deformability in patients with retinal vein occlusion

Becatti

Marcucci

Gori

et al. 2016

Journal of Thrombosis and Haemostasis

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Essentials• Retinal vein occlusion (RVO), characterized by blood hyperviscosity, has an unclear pathogenesis.• We aimed to find out if hemorheological profile is altered by oxidative stress in RVO patients.• Red blood cell (RBC) oxidative stress is associated to whole blood viscosity and RBC deformability.• Reactive oxygen species alter RBC membrane rigidity, playing a key role in RVO pathogenesis.Summary. Background: Retinal vein occlusion (RVO) is characterized by vision loss resulting from hypoperfusion and hypoxia of the retina. RVO pathogenesis is not yet fully understood, although blood hyperviscosity has been observed. Erythrocyte deformability plays a key role in determining blood viscosity, and it is critical to microvascular perfusion and oxygen delivery. It has been shown that oxidative stress-induced erythrocyte membrane fluidity alterations are linked to the progression of cardiovascular diseases. Objectives: To determine whether erythrocytes from RVO patients show signs of oxidative stress, and whether this condition can modify the hemorheologic profile in these patients. Patients and Methods: We analyzed the entire hemorheologic profile and erythrocyte oxidative stress -reactive oxygen species (ROS) production and membrane lipid peroxidation -in 128 RVO patients and 128 healthy subjects, matched for age and sex. Fluorescence anisotropy was used to evaluate the fluidity of erythrocyte membranes. Results: In RVO patients, erythrocyte oxidative stress was present and positively correlated with whole blood viscosity and erythrocyte deformability. Multivariate linear regression analysis after adjustment for age, cardiovascular risk factors, medications, leukocyte number and mean corpuscular volume indicated that erythrocyte-derived ROS and erythrocyte lipid peroxidation were significantly and positively correlated with erythrocyte membrane viscosity and deformability. Moreover, in vitro experiments demonstrated that ROS have a key role in erythrocyte membrane fluidity. Conclusions: Our findings indicate that erythrocyte oxidative stress plays a key role in the pathogenesis of RVO, and pave the way to new therapeutic interventions.

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Increased homocysteine, homocysteine-thiolactone, protein homocysteinylation and oxidative stress in the circulation of patients with Eales’ disease

Cited by 9 publications

References 42 publications

Homocysteine and risk of age‐related macular degeneration: a systematic review and meta‐analysis

Homocysteine and risk of age‐related macular degeneration: a systematic review and meta‐analysis

Homocysteine & its metabolite homocysteine-thiolactone & deficiency of copper in patients with age related macular degeneration - A pilot study

Erythrocyte oxidative stress is associated with cell deformability in patients with retinal vein occlusion

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