Increased human immunodeficiency virus (HIV) expression in chronically infected U937 cells upon in vitro differentiation by hydroxyvitamin D3: roles of interferon and tumor necrosis factor in regulation of HIV production

Locardi, C; Petrini, Carlo; Boccoli, Giovanni; Testa, Ugo; Diefenbach, C; Buttò, Stefano; Belardelli, Filippo

doi:10.1128/jvi.65.3.1666-.1991

Cited by 19 publications

(25 citation statements)

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“…A similar adverse effect on the U937 line was shown in an experiment in which vitamin D incubation promoted both macrophage-like differentiation and increased HIV production. 64 1,25(OH) 2 D also increased HIV replication in studies of promyelocytic/ myeloblastic HL-60 cells 65 and in A3.5 cells and peripheral blood monocytes that had been acutely infected with HIV. 66 Vitamin D analogs enhanced HIV-1 replication in proportion to their affinity for the VDR.…”

Section: In Vitro Experimentsmentioning

confidence: 91%

A Potential Role for Vitamin D on HIV Infection?

Villamor¹

2006

Nutrition Reviews

121

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Despite advances in the knowledge of vitamin D's potent immunomodulatory activity, its role on HIV disease progression is unknown. Decreased concentrations of 1␣,or 1,25(OH) 2 D, the active form of vitamin D, have been reported among HIV-infected people and attributed to defects in renal hydroxylation and increased utilization. A few studies also described low levels of 25-hydroxyvitamin D 3 , 25(OH)D, the vitamin obtained from solar synthesis and diet. An inverse association between 1,25(OH) 2 D concentrations and mortality has been reported from a small cohort of HIV-infected adults, and some cross-sectional studies have indicated positive correlations between 1,25(OH) 2 D and CD4 ϩ cell counts. Additional observational studies are needed to confirm the associations between vitamin D status and HIV disease progression. These investigations would provide useful insights on the potential role of vitamin D supplementation to HIV-infected persons and the planning of intervention trials.

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Section: In Vitro Experimentsmentioning

confidence: 91%

A Potential Role for Vitamin D on HIV Infection?

Villamor¹

2006

Nutrition Reviews

121

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“…Of note is the observation that certain cytokines that have been demonstrated to induce HIV expression in monocytic cells in vitro, such as tumor necrosis factor-alpha (TNF-␣) and interleukin-6 (IL-6), have been found at elevated levels in HIV-infected individuals compared with those in healthy controls (3,6,20). Numerous in vitro studies have investigated the role of cellular differentiation in modulating HIV production (9,17,19,26,31,42,44); however, the results have been somewhat conflicting. Possible explanations for these inconsistencies may be the different states of maturation of the monocytic cells under study, as well as the presence or absence of particular HIV-inducing cytokines.…”

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confidence: 99%

Effect of cellular differentiation on cytokine-induced expression of human immunodeficiency virus in chronically infected promonocytic cells: dissociation of cellular differentiation and viral expression

Goletti

Kinter

Biswas

et al. 1995

J Virol

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Cellular differentiation is thought to play an important role in the susceptibility of monocytic lineage cells to human immunodeficiency virus (HIV) infection as well as in their ability to support virus replication. In addition, virus replication in monocytes/macrophages has been demonstrated in vitro to be strongly modulated by several cytokines such as tumor necrosis factor alpha and granulocyte-macrophage colony-stimulating factor. The purpose of the present study was to investigate the interaction between cellular differentiation and cytokines in the regulation of HIV expression from chronically infected monocytic lineage cells. U1, a persistently HIV-infected promonocytic cell line, is characterized by low levels of virus expression which can be modulated by several cytokines. 1␣,-25-Dihydroxyvitamin D3 (Vit.D3), a well-known differentiating agent for myelomonocytic cells which has been previously reported to modulate HIV replication in other in vitro systems, induced maturation of U1 cells toward a macrophage-like phenotype, as demonstrated by the induction of the differentiation-associated cell surface markers CD14 and CD11b. Vit.D3-induced differentiation did not result in induction of HIV expression; however, when U1 cells were stimulated with tumor necrosis factor alpha in the presence of Vit.D3, a synergistic induction of cell differentiation and viral expression was demonstrated. In contrast, Vit.D3 suppressed the induction of HIV expression in U1 cells stimulated with gamma interferon, interleukin-6, and granulocyte-macrophage colony-stimulating factor, although synergy between Vit.D3 and these cytokines was observed in terms of cellular differentiation. These data suggest that differentiation of monocytic cells does not necessarily correlate with increased HIV expression.

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“…Several studies have now examined the relationship among HIV-1 infection of myeloid cells, induction of NF-KB binding activity (2,23,47,50,64), cellular differentiation (1,17,46), and HIV-1 gene expression (38,67). Previously, we established a population of HIV-1-infected myelomonoblastic cells (PLB-IIIB) derived from the PLB-985 cell line (55).…”

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confidence: 99%

Chronic human immunodeficiency virus type 1 infection stimulates distinct NF-kappa B/rel DNA binding activities in myelomonoblastic cells

Roulston

Beauparlant

Rice

et al. 1993

J Virol

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The relationship between human immunodeficiency virus type 1 (HlI-1) infection and the induction of NF-KB binding activity was examined in a myeloid cell model of HIV-1 infection derived from the PLB-985 cell line. Chronic infection of PLB-985 cells led to increased monocyte-specific surface marker expression, increased c-Jins gene transcription, and morphological alterations consistent with differentiation along the monocytic pathway. PLB-IiB cells displayed a constitutive NF-KB-like binding activity that was distinct from that induced by tumor necrosis factor alpha or phorbol 12-myristate 13-acetate treatment of the parental PLB-985 cell line. This unique DNA binding activity consisted of proteins of 70, 90, and 100 kDa with a high degree of binding specificity for the NF-cB site within the PRDII domain of beta interferon. In this report, we

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Increased human immunodeficiency virus (HIV) expression in chronically infected U937 cells upon in vitro differentiation by hydroxyvitamin D3: roles of interferon and tumor necrosis factor in regulation of HIV production

Cited by 19 publications

References 0 publications

A Potential Role for Vitamin D on HIV Infection?

A Potential Role for Vitamin D on HIV Infection?

Effect of cellular differentiation on cytokine-induced expression of human immunodeficiency virus in chronically infected promonocytic cells: dissociation of cellular differentiation and viral expression

Chronic human immunodeficiency virus type 1 infection stimulates distinct NF-kappa B/rel DNA binding activities in myelomonoblastic cells

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