Increased IL-6 gene expression and production in patients with common variable immunodeficiency

Abstract

SUMMARYWe have studied IL-6 gene expression and production by in vitro stimulated peripheral blood mononuelear cells (PBMC) isolated from common variable immunodeficieney (CVI) patients, A strong hybridization signal for the lL-6 probe was observed in mRNA extraeted from phytohaemaggiutinin (PHA)-and PHA/phorbol myristate acetate (PMA)-stimulated PBMC from most of 12 CVI patients analysed, IL-6 production by PHA-stimuIated PBMC from 28 CVI patients was evaluated in FLISA and found to be significantly (/'<0()()0]) higher than in normal controls, IL-6 production, however, did not correlate with the lymphocyte populations examined, nor with the absolute number of monocytes. We have also showed that IL-6 was able to inerease IgM secretion by several Epstein-Barr virus (EBV)-transformed cell lines derived from both normal donors and CVI patients, but it failed to modify substantially the amounts of IgM and IgG produced in vitro by PBMC derived from CVI patients and activated with pokcweed miVogen (PWM) or anti-lgM, Our data indicate that lL-6 gene expression and production is increased in CVI, but CVI cells do not respond to IL-6 with increased production of immunoglobulin.

Adiponectin Receptors and Pro-inflammatory Cytokines Are Modulated in Common Variable Immunodeficiency Patients: Correlation With Ig Replacement Therapy

“…Indeed, changes in the cytokine expression are evident only after several Ig administrations, as observed in patients on maintenance therapy. IL-6 and IL-8 are mainly produced and secreted by macrophages thereby enhancing the proliferation and the differentiation of B cells into memory or plasma cells (33, 34), while IL-2 and IL-4 are produced in most part by T lymphocytes (35). The up-regulation of IL-6 and IL-8 in CVID maintenance therapy may indicate a primary involvement of macrophages instead of T lymphocytes.…”

Seeking Relevant Biomarkers in Common Variable Immunodeficiency

“…However, in a larger cohort, we had found abundant serum IFN-γ in CVID+ patients, along with an IFN mRNA signature that distinguished CVID+ from CVID patients and healthy volunteers (further discussed below) ( 27 , 28 ). A number of other cytokines had also been examined in CVID, though the results often varied between studies: IL-4 (elevated serum levels in most studies, but reduced in some) ( 29 , 30 ), IL-5 (potentially reduced) ( 31 ), IL-6 (increased production in some studies) ( 32 , 33 ), IL-7 (varied) ( 34 ), IL-10 (varied) ( 35 , 36 ), and IL-12 (varied) ( 37 , 38 ).…”