Increased incidence of DNA amplification in follicular than in uterine and blood samples indicates possible tropism of Neospora caninum to the ovarian follicle

Silva, Andressa F.; Rangel, Lucía; Ortiz, Carlos García; Salinas, Elizabeth Morales; Zanella, Eraldo Lourenso; Castillo-Velázquez, Uziel; Gutiérrez, C. M.

doi:10.1016/j.vetpar.2012.02.013

Cited by 10 publications

(6 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Despite suggestions of tropism of N. caninum for ovarian follicles (Silva et al, 2012), in our experimental conditions, N. caninum infection had no clear effects on the immune response taking place in the corpus luteum. However, the significant positive correlation between the luteal expression of Treg (IL10) and the expression of Th1 (IFNγ) and Th2…”

Section: Discussioncontrasting

confidence: 87%

“…Despite suggestions of tropism of N. caninum for ovarian follicles (Silva et al., ), in our experimental conditions, N. caninum infection had no clear effects on the immune response taking place in the corpus luteum. However, the significant positive correlation between the luteal expression of Treg ( IL10 ) and the expression of Th1 ( IFN γ ) and Th2 ( IL4) in both luteal and placental tissues suggests that the placenta can act as an immune organ that can sense changes in the environment and then signal to other cells to elicit a response (Schminkey & Groer, ).…”

Section: Discussioncontrasting

confidence: 85%

See 1 more Smart Citation

Uterine serpin (SERPINA 14) correlates negatively with cytokine production at the foetal–maternal interface but not in the corpus luteum in pregnant dairy heifers experimentally infected with Neospora caninum

Serrano-Pérez

Almerı́a

Mur-Novales

et al. 2017

Reprod Domestic Animals

View full text Add to dashboard Cite

This study examines gene expression patterns in dairy heifers experimentally infected with N. caninum during on Day 110 of pregnancy with live foetuses at euthanasia, 42 days later. The study population was constituted of four non-infected controls and three infected dams. Gene expression was determined on gamma interferon (IFNγ), (Th1 pro-inflammatory cytokine), interleukin-4 (IL4) (Th2 pro-gestation cytokine) or interleukin-10 (IL10) (T regulatory cytokine) and the serine peptidase inhibitor SERPINA14 in intercaruncular, placental, uterine lymph node (UTLN) and luteal tissue samples. Intercaruncular SERPINA14 expression was negatively correlated with IFNγ expression in cotyledon samples and with IL4 expression in UTLN. No relationships were detected between cytokine gene expression at the foetal-maternal interface and SERPINA14 expression in the luteal samples. Our findings suggest that gene expression of the uterine serpin SERPINA14 correlates negatively with the expression of Th1 and Th2 cytokines at the foetal-maternal interface but not in the corpus luteum.

show abstract

Section: Discussioncontrasting

confidence: 87%

Section: Discussioncontrasting

confidence: 85%

Uterine serpin (SERPINA 14) correlates negatively with cytokine production at the foetal–maternal interface but not in the corpus luteum in pregnant dairy heifers experimentally infected with Neospora caninum

Serrano-Pérez

Almerı́a

Mur-Novales

et al. 2017

Reprod Domestic Animals

View full text Add to dashboard Cite

show abstract

“…Tritrichomonas foetus has been reported to damage oocytes (Benchimol et al, 2007). Also, N. caninum has been detected in bovine follicles during assisted reproduction manipulations (Silva et al, 2012), as well as in bovine foetuses (Marques et al, 2011), although there are reports suggesting that embryo transfer is a safe method to avoid vertical transmission of these protozoon (Landmann et al, 2002;Moskwa et al, 2008). These implications should be borne in mind, as assisted reproductive technologies for sheep are developing and being applied in larger numbers of animals (Amiridis and Cseh, 2012).…”

Section: Number Of Ovulations and Conceptionmentioning

confidence: 99%

Interactions between parasitic infections and reproductive efficiency in sheep

Fthenakis

Mavrogianni

Gallidis

et al. 2015

Veterinary Parasitology

View full text Add to dashboard Cite

This review article summarises the many reports in the literature, confirming that, in sheep, parasitic infections can adversely affect reproductive efficiency; examples, which refer to all parts of the reproductive cycle of sheep, are as follows: trichostrongylosis in ewe-lambs (which can lead to delayed attainment of puberty), myiosis of the prepuce (which can cause impediment of mating), chorioptic mange or trypanosomosis in rams (which can lead to testicular degeneration or azoospermia, respectively), trypanosomosis or sarcoptic mange in pre-conceptual ewes (which can lead to poor conception rates or reduced number of ovulations, respectively), toxoplasmosis or neosporosis in pregnant ewes (which are causes of abortion), trichostrongylosis or trematode infections in lactating ewes (which can cause reduction of milk yield and can be a risk factor for mastitis, respectively), cryptosporidiosis in newborn lambs (which can be a cause of deaths), coccidiosis in growing pre-weaned lambs (which can cause suboptimal growth rate). In other cases, the reproductive status of the animal can influence the parasitic infection; examples are as follows: the increase in faecal parasitic output during the peri-parturient period (as a consequence of the peri-parturient relaxation of immunity), the heavier trichostrongylid infections of twin lambs compared to lambs from single parities (as a consequence of developmental origin issues in twin lambs). All the above examples support the idea of presence of interactions between parasitic infections and reproductive efficiency in sheep.

show abstract

“…The endometrium is particularly susceptible to microbial infections, increasing the risk of adverse pregnancy outcomes [31]. Neospora caninum tachyzoites have been detected in the uterus of naturally infected animals, and N. caninum tissue cysts have also been found in the endometrium and the maternal-fetal interface (crypt) [32][33][34]. The objectives of the present study were to investigate the expression profiles of lncRNAs and messenger RNAs (mRNAs) in caprine endometrial epithelial cells (EECs) during N. caninum infection.…”

Section: Introductionmentioning

confidence: 99%

RNA sequencing reveals dynamic expression of lncRNAs and mRNAs in caprine endometrial epithelial cells induced by Neospora caninum infection

et al. 2022

View full text Add to dashboard Cite

Background The effective transmission mode of Neospora caninum, with infection leading to reproductive failure in ruminants, is vertical transmission. The uterus is an important reproductive organ that forms the maternal–fetal interface. Neospora caninum can successfully invade and proliferate in the uterus, but the molecular mechanisms underlying epithelial-pathogen interactions remain unclear. Accumulating evidence suggests that host long noncoding RNAs (lncRNAs) play important roles in cellular molecular regulatory networks, with reports that these RNA molecules are closely related to the pathogenesis of apicomplexan parasites. However, the expression profiles of host lncRNAs during N. caninum infection has not been reported. Methods RNA sequencing (RNA-seq) analysis was used to investigate the expression profiles of messenger RNAs (mRNAs) and lncRNAs in caprine endometrial epithelial cells (EECs) infected with N. caninum for 24 h (TZ_24h) and 48 h (TZ_48 h), and the potential functions of differentially expressed (DE) lncRNAs were predicted by using Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of their mRNA targets. Results RNA-seq analysis identified 1280.15 M clean reads in 12 RNA samples, including six samples infected with N. caninum for 24 h (TZ1_24h-TZ3_24h) and 48 h (TZ1_48h-TZ3_48h), and six corresponding control samples (C1_24h-C3_24h and C1_48h-C3_48h). Within the categories TZ_24h-vs-C_24h, TZ_48h-vs-C_48h and TZ_48h-vs-TZ_24h, there were 934 (665 upregulated and 269 downregulated), 1238 (785 upregulated and 453 downregulated) and 489 (252 upregulated and 237 downregulated) DEmRNAs, respectively. GO enrichment and KEGG analysis revealed that these DEmRNAs were mainly involved in the regulation of host immune response (e.g. TNF signaling pathway, MAPK signaling pathway, transforming growth factor beta signaling pathway, AMPK signaling pathway, Toll-like receptor signaling pathway, NOD-like receptor signaling pathway), signaling molecules and interaction (e.g. cytokine-cytokine receptor interaction, cell adhesion molecules and ECM-receptor interaction). A total of 88 (59 upregulated and 29 downregulated), 129 (80 upregulated and 49 downregulated) and 32 (20 upregulated and 12 downregulated) DElncRNAs were found within the categories TZ_24h-vs-C_24h, TZ_48h-vs-C_48h and TZ_48h-vs-TZ_24h, respectively. Functional prediction indicated that these DElncRNAs would be involved in signal transduction (e.g. MAPK signaling pathway, PPAR signaling pathway, ErbB signaling pathway, calcium signaling pathway), neural transmission (e.g. GABAergic synapse, serotonergic synapse, cholinergic synapse), metabolism processes (e.g. glycosphingolipid biosynthesis-lacto and neolacto series, glycosaminoglycan biosynthesis-heparan sulfate/heparin) and signaling molecules and interaction (e.g. cytokine-cytokine receptor interaction, cell adhesion molecules and ECM-receptor interaction). Conclusions This is the first investigation of global gene expression profiles of lncRNAs during N. caninum infection. The results provide valuable information for further studies of the roles of lncRNAs during N. caninum infection. Graphical Abstract

show abstract

Increased incidence of DNA amplification in follicular than in uterine and blood samples indicates possible tropism of Neospora caninum to the ovarian follicle

Cited by 10 publications

References 31 publications

Uterine serpin (SERPINA 14) correlates negatively with cytokine production at the foetal–maternal interface but not in the corpus luteum in pregnant dairy heifers experimentally infected with Neospora caninum

Uterine serpin (SERPINA 14) correlates negatively with cytokine production at the foetal–maternal interface but not in the corpus luteum in pregnant dairy heifers experimentally infected with Neospora caninum

Interactions between parasitic infections and reproductive efficiency in sheep

RNA sequencing reveals dynamic expression of lncRNAs and mRNAs in caprine endometrial epithelial cells induced by Neospora caninum infection

Contact Info

Product

Resources

About