Increased Incidence of Urethane Induced Lung Adenomata by Autosensitized Lymphocytes

Levo, Y; Carnaud, Claude; Cohen, Irun R.; Trainin, Nathan

doi:10.1038/bjc.1974.72

Cited by 6 publications

(2 citation statements)

References 15 publications

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“…Since autosensitized lymphocytes augmented the number of urethane-induced lung adenomas, Levo et al (1974) suggested that the host immune system was inadequately responsive, the effect being systemic rather than local. The possibility that immunosuppression by urethane could be counteracted by a-irradiation, and so lead to reduced adenoma formation, was considered by Brightwell and Heppleston (1973).…”

Section: Discussionmentioning

confidence: 99%

Effect of inhaled plutonium dioxide on development of urethane-induced pulmonary adenomas

Brightwell¹,

Heppleston²

1977

Br J Cancer

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Summary.-Mice were exposed to plutonium dioxide (PuO 2) aerosols 2 weeks before or after urethane injection. Both exposures reduced the number and size of adenomas. The incidence of arrested metaphases showed no consistently significant differences between plutonium-exposed and mock-exposed animals.The results are discussed in relation to recent electron microscopic evidence of degenerative changes in the type II epithelial cells of the mouse lung following PuO2 inhalation. It is concluded that damage at the cellular level may account for the observed reduction in growth of pulmonary adenomas in mice whose lungs contained plutonium particles.THE INCIDENCE of pulmonary adenomas in urethane-treated mice is decreased by large doses of whole-body X-irradiation (Foley and Cole, 1963;Bartlett, 1970). Inhalation of 239PuO 2 particles 2 weeks before urethane injection led to a significant decrease in tumour incidence (Brightwell and Heppleston, 1973). The proliferative response in the tumours has now been examined as well as the effect of Pu inhalation after uretbane administration. MATERIALS AND METHODSRandom-bred, male A2G mice 4 to 7 mnonths old were used. Animals were divided into 4 age-matched groups, each of wrhich contained 57 animals in Experiment 1 and 26 in Experiment 2, the treatments being as follows: Experiment 1.-(a) Plutonium inhalation followed by urethane (PU); (b) plutonium inhalation followed by saline (PS); (c) mock inhalation followed by urethane (MU); (d) mock inhalation followed by saline (MS). Experiment 2.-(a) Urethane followed by plutonium inhalation (UP); (b) saline followed by plutonium inhalation (SP); (c) urethane followed by mock inhalation (UM); (d) saline followed by mock inhalation (SM).An aerosol of 239PuO2 was generated by an exploding-wire technique, and inhalation took place in a specially devised chamber with the mice partially enclosed in glass tubes (Brightwell and Carter, 1976). The average lung deposit was estimated to be 925 Bq (25 nCi). Mock exposure of inice similarly enclosed in glass tubes -was carried out in the laboratory atmosphere. Urethane (1 mg/g body weight) or saline was injected i.p. 2 weeks before or after inhalation exposure. Mice also received an i.p. injection of vinblastine sulphate (4 ,tg/g body weight) 4 h and [3H] thymidine (1-85 x 104 Bq [0-5 jrCil/g body weight) 1 h before death.Groups of 6 or 7 mice were killed by cervical dislocation at intervals up to 37 weeks after urethane or saline injection, the animals remaining healthy throughout. The lungs were fixed in Carnoy's fluid and tumours on the surfaces of both lungs were counted and measured using a dissecting microscope. From the left lung, 5-,tm paraffin sections were either stained by the periodic acid-Schiff/haematoxylin method or used for autoradiography with Kodak AR 10 stripping film. The mitotic incidence (MI) following metaphase arrest for a nominal 4 h and the labelling incidence (LI) after 1 h were estimated in individual tumours. Unless stated otherwise, statistical significance was determ...

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Section: Discussionmentioning

confidence: 99%

Effect of inhaled plutonium dioxide on development of urethane-induced pulmonary adenomas

Brightwell¹,

Heppleston²

1977

Br J Cancer

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“…With in vitro sensitization, animal immunotherapy trials showed that when these sensitized cells were mixed with live tumor cells and injected locally, they sometimes had an enhancing effect on the tumor growth [22,7,17,11], but if given IV they were effective in preventing the tumor growth [21,8]. The in vitro-sensitized lymphocytes were able to prevent recurrence of tumors when the primary tumor was removed, and this improved the survival by 40%.…”

Section: Introductionmentioning

confidence: 99%

In vitro autologous blast cell sensitization as immunotherapy in acute non-lymphocytic leukemia

Denegri

Grossman

Morgan

et al. 1980

Cancer Immunol Immunother

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Kinetics of the response of spleen cells from tumor‐bearing animals in an in vivo tumor neutralization assay

Gabizón

Small

Trainin

1976

Intl Journal of Cancer

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The behavior of spleen cells from tumor-bearing mice, vis-à-vis isologous tumor cells, was investigated by means of an in vivo adoptive neutralization test. C3H/eB mice were challenged with tumor cells from a chemically induced fibrosarcoma. Spleens from these animals were removed at weekly intervals following tumor inoculation, mixed with tumor cells, and tested for their influence on tumor growth in syngeneic recipient mice. Two phases in the reactivity of spleen cells from tumor-bearing mice were clearly distinguishable. In a first stage of tumor growth, these mice yielded specific tumor-inhibitory cells conferring protection. Subsequently, the protective activity declined leading to a second phase characterized by tumor enhancement. Both protective and enhancing activities were shown to be mainly dependent on the presence of T cells.

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Increased Incidence of Urethane Induced Lung Adenomata by Autosensitized Lymphocytes

Cited by 6 publications

References 15 publications

Effect of inhaled plutonium dioxide on development of urethane-induced pulmonary adenomas

Effect of inhaled plutonium dioxide on development of urethane-induced pulmonary adenomas

In vitro autologous blast cell sensitization as immunotherapy in acute non-lymphocytic leukemia

Kinetics of the response of spleen cells from tumor‐bearing animals in an in vivo tumor neutralization assay

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