Increased Inhibition of Angiotensin Converting Enzyme (ACE) Obtained from Indonesian Buffalo Meat Protein Using SEP-PAK Plus C18

Jamhari,; S, Rio Olympias; Suryanto, Edi; Rusman,; Setiyono,; Triyannant, Endy; Erwanto, Y.; Yuliatmo, Ragil

doi:10.3923/pjn.2018.434.440

Cited by 2 publications

(2 citation statements)

References 26 publications

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“…The enzymes used for hydrolysis include pepsin, trypsin, and chymotrypsin (Sujarwanto et al, 2018;Winarti et al, 2019). The hydrolysis using various digestive enzymes have shown to be able to produce bioactive peptide compounds as antihypertensive agents from the animal products such as meat of buffalo (Sujarwanto et al, 2018), foot of chicken (Bravo et al, 2019), milk of goat (Widodo et al, 2019), the meat of rabbit (Permadi et al, 2019), and meat of duck (Winarti et al, 2019). The bioactive peptide is able to inhibit the activity of angiotensin converting enzyme in the RAAS system (Renin-Angiotensin-Aldosterone System), which causes hypertension due to the constriction of blood vessels (Hsueh and Wyne, 2011).…”

Section: Extraction Of Collagen From the Skin Of Kacang Goat And Production Of Itsmentioning

confidence: 99%

Extraction of Collagen from the Skin of Kacang Goat and Production of Its Hydrolysate as an Inhibitor of Angiotensin Converting Enzyme

Hakim

Pratiwi

Jamhari

et al. 2021

Trop. Anim. Sci. J.

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The study was designed to determine the potential of collagen hydrolysate produced from the skin of Kacang goat through chymotrypsin hydrolysis to be used as an inhibitor of angiotensin converting enzyme (ACE). This research was conducted in three replications, with the measured parameters include ACE inhibitory potential and collagen hydrolysate fractionation. The results showed that collagen extraction of Kacang goat skin by chymotrypsin hydrolysis yielded 9.74% (dry matter, v/v) collagen, with pH at 6.6. The extracted collagen contained α1, α2, and β collagen chains with molecular weights of 151 kDa, 141 kDa, and 240 kDa, respectively. Furthermore, the collagen hydrolysis produced protein peptides confirmed at molecular weights of 43 to 107 kDa. The hydrolysate fractionation at molecular weights of <3 kDa, 3-5 kDa, and >5 kDa showed proteins concentrations of 2.33 mg/mL, 3.81 mg/mL, and 3.93 mg/mL, respectively. The hydrolysate fractionation with molecular weight <3 kDa showed to have ACE inhibition activity with the IC50 value of 0.47 mg/mL. The study concluded that collagen hydrolysate extracted from the skin of Kacang goat had a promising potential as a source of antihypertensive agent.

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Section: Extraction Of Collagen From the Skin Of Kacang Goat And Production Of Itsmentioning

confidence: 99%

Extraction of Collagen from the Skin of Kacang Goat and Production of Its Hydrolysate as an Inhibitor of Angiotensin Converting Enzyme

Hakim

Pratiwi

Jamhari

et al. 2021

Trop. Anim. Sci. J.

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“…Various biological activities of bioactive peptides derived from enzymatic hydrolysis of bubaline casein have been described, especially in the beta fraction, including inhibition of angiotensin-converting enzyme (Abdel-Hamid, et al, 2017;Sujarwanta, et al, 2018) and antioxidant (Shazly, et al, 2017;Shazly, et al, 2019) and antimicrobial activities (Nielsen, et al, 2017;Zhao, et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant activity, inhibition of angiotensin I converting enzyme (ACE) and antibacterial activity of buffalo caseinate protein hydrolysates and their fractions

Santos

Silva²,

Nascimento

et al. 2020

RSD

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In the present study, buffalo milk caseinate hydrolysates produced by bromelain, neutrase, papain and trypsin were ultra-filtered and different fractions were assessed for antioxidant, inhibition of angiotensin converting enzyme and antimicrobial activity. Biological potential was assessed by a number of metrics: ability to remove radicals of 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid), 2,2'-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyls; copper and iron chelation; antidiabetic properties; antihypertensive assay; and antibacterial activity against Escherichia coli ATCC 25922, Listeria monocytogenes ATCC 19114, Salmonella typhimurium ATCC 14028 and Staphylococcus aureus ATCC 25923 strains. The tests for scavenging of hydroxyl radicals and DPPH revealed a greater potential in the 3–10 kDa fractions. Iron chelation activity >70% was observed in all the fractions, including <3 kDa. Copper chelation was >60% in fractions >10 kDa. α-Amylase inhibition and antihypertensive activity was optimal in the <3 kDa fraction. Antibacterial activity ranged between 3.28 and 100% inhibition against microorganisms tested, the fraction <3 kDa showed a greater inhibitory potential. The antihypertensive activity of fractions ranged between 39.35 and 89.58%. All treatments were able to produce hydrolysates and fractions with biological potential and, so the ultrafiltration method proved to be effective in the separation of peptides with different molar masses and potential use in the food or pharmaceutical industry.

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Increased Inhibition of Angiotensin Converting Enzyme (ACE) Obtained from Indonesian Buffalo Meat Protein Using SEP-PAK Plus C18

Cited by 2 publications

References 26 publications

Extraction of Collagen from the Skin of Kacang Goat and Production of Its Hydrolysate as an Inhibitor of Angiotensin Converting Enzyme

Extraction of Collagen from the Skin of Kacang Goat and Production of Its Hydrolysate as an Inhibitor of Angiotensin Converting Enzyme

Antioxidant activity, inhibition of angiotensin I converting enzyme (ACE) and antibacterial activity of buffalo caseinate protein hydrolysates and their fractions

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