Increased interleukin-10 associated with low IL-6 concentration correlated with greater survival rates in mice infected by rabies virus vaccinated against it and immunomodulated with P. acnes

Megid, Jane; Kaneno, Ramon; Nozaki, Cristiane Nakada; Brito, Cjc; Almeida, Marilene Fernandes de

doi:10.1016/j.cimid.2004.01.002

Cited by 7 publications

(6 citation statements)

References 25 publications

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“…in only rabies infected group as well as infected-vaccinated group as compared to control mice. However, lower levels of IL-6 were observed, together with greater survival rates in the group infected, vaccinated and treated with an immunomodulator such as Propionibacterium acnes [18,19]. It is evident that though viral infection and in some cases vaccination too, both by themselves may be capable of stimulating the cytokine response, introduction of an immunomodulator in combination can significantly alter the gene expression, at times leading to even downregulation or inhibition that may prove beneficial to the host.…”

Section: Discussionmentioning

confidence: 99%

Exogenous interferon prolongs survival of rabies infected mice

et al. 2015

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Rabies is an acute viral infection that causes encephalomyelitis in almost all warm blooded animals and is invariably fatal once the clinical signs appear. The present study was carried out to assess the effect of recombinant human interferon alpha (rhIFN a-2A) treatment on the survival of rabies infected mice and its correlation with cytokines expression. The gene expression of TNF-a and IL-6 was measured by SYBR Green Real Time PCR for two groups-''Pre-exposure'' (mice were inoculated with rhIFN a-2A prior to rabies infection) and ''Post-exposure'' (mice were inoculated with rhIFN a-2A post rabies virus infection). Delayed mortality was observed in interferon treated infected groups. In addition, statistically significant decrease (P \ 0.0001) in the expression of TNF-a and IL-6 was observed, both in the pre-exposure and post-exposure groups. These findings indicate that modulation of cytokine secretion using exogenous biologicals such as rhIFN may offer novel therapeutic approaches to treat diseases such as rabies.

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Section: Discussionmentioning

confidence: 99%

Exogenous interferon prolongs survival of rabies infected mice

et al. 2015

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“…Higher levels of IFN-γ production have been observed in rabies vaccinated mice when given inactivated P. acnes compared to mice without P. acnes or other potential immunstimulants including avridine and BCG [25]. The mechanism of action of P. acnes appears to be both on the macrophages and natural killer cells [25,26]. Similar immune stimulation is required for immunity to tuberculosis, and leads to a potential role of Propionibacterium sp.…”

Section: Introductionmentioning

confidence: 97%

“…as an adjuvant for such a vaccine. P. acnes is however, a potential pathogen, and efficacy to date has been demonstrated by inactivated organism given via either the intra-venous or intra-peritoneal route [15,25,26].…”

Section: Introductionmentioning

confidence: 99%

The efficacy of Propionibacterium jensenii 702 to stimulate a cell-mediated response to orally administered soluble Mycobacterium tuberculosis antigens using a mouse model

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Hitchick

et al. 2005

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-Mucosal vaccination using adjuvant protein vaccines may offer a novel approach for tuberculosis. To date, however, development of such a vaccine has been considered unlikely due to the inability to identify a safe adjuvant that stimulates an appropriate immune response. This study was undertaken to determine the potential of a dairy propionibacteria, P. jensenii strain 702 (PJ702), to act as an adjuvant when co-administered orally with soluble tuberculosis protein to mice. The efficacy of the PJ702 to act as an adjuvant was assessed by comparison with cholera toxin. C57 mice were orally immunized with Mycobacterium tuberculosis short-term culture filtrate protein (STCF) (200 µg) with either PJ702 (10 8 cfu) or cholera toxin (10 µg) in a total volume of 100 µL. A control group was given PJ702 10 8 cfu alone. Each mouse (eight per group) was vaccinated weekly over a 21-day period. At day 25 the mice were sacrificed, spleens were collected and lymphocyte cultures prepared. After stimulation with STCF (2.5 µg), cell proliferation was measured by 3 H thymidine uptake, and the cytokines, IL-4 and IFN-γ , by ELISA. A significantly higher T-cell proliferation was observed for the group given the vaccine containing PJ702, compared to both the control group and the group given the vaccine containing cholera toxin (P < 0.05). The predominant cytokine produced from all groups was IFN-γ . These results indicate potential for future development of an oral tuberculosis vaccine, and also identify PJ702 as a potential living vaccine vector that could be applied to a number of mucosally transmitted diseases. PJ702 (10 8 ufc) ou avec la toxine cholérique (10 µg), dans un volume total de 100 µL. Un groupe témoin a reçu PJ702 seul (10 8 ufc). Chaque souris (8 par groupe) a été vaccinée hebdomadairement sur une période de 21 jours. Le 25 e jour, les souris ont été sacrifiées, la rate prélevée et les lymphocytes mis en culture. Après stimulation par STCF (2,5 µg), la prolifération cellulaire a été évaluée par mesure de l'incorporation de la thymidine 3 H ; la production des cytokines, IL-4 et IFN-γ , a été évalué par ELISA. Une prolifération des cellules T significativement plus élevée a été observée dans le groupe recevant le vaccin contenant PJ702, par rapport au groupe témoin, ou au groupe recevant le vaccin contenant la toxine cholérique (P < 0,05). La cytokine majoritairement produite dans tous les groupes était IFN-γ . Ces résultats montrent le potentiel de développement d'une stratégie de vaccination orale contre la tuberculose. PJ702 est identifié comme un vecteur potentiel pour délivrer des vaccins vivants, applicable aux différents types de maladies d'origine mucosale.Propionibacterium / tuberculose / vaccin / réponse immunitaire / adjuvant

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“…P. acnes is a potent stimulator of the reticuloendothelial system (RES) (Megid & Kaneno 2000, Megid et al 2004, Tizard 2009) that enhanced both macrophage and lymphocyte function, natural killer cytotoxicity and cytokine production in laboratory animals. It has been recognised that P. acnes contribute the induction of pro-inflammatory cytokines IL-1α, IL-1β, IL-8 and TNF-α (Perry & Lambert 2006) and increase IL-10 in mice infected with street rabies virus (Megid et al 2004). P. acnes is able to modulate the late phase reaction of the type I hypersensitivity in rodents and it has capacity to potentiate or suppress the Th2 response to OVA challenge, depending on the treatment protocol used (Braga et al 2003).…”

Section: Effect Of Oral Administration Ofmentioning

confidence: 99%

“…Propionibacterium acnes, formerly known as Corynebacterium parvum, is a non-spore Gram positive bacteria and is considered an opportunistic pathogens (Perry & Lambert 2006). P. acnes is a potent stimulator of the reticuloendothelial system (RES) (Megid & Kaneno 2000, Megid et al 2004, Tizard 2009) that enhanced both macrophage and lymphocyte function, natural killer cytotoxicity and cytokine production in laboratory animals. It has been recognised that P. acnes contribute the induction of pro-inflammatory cytokines IL-1α, IL-1β, IL-8 and TNF-α (Perry & Lambert 2006) and increase IL-10 in mice infected with street rabies virus (Megid et al 2004).…”

Section: Effect Of Oral Administration Ofmentioning

confidence: 99%