Increased interleukin-6 concentrations in cervical secretions are associated with preterm delivery

Lockwood, Charles J.; Ghidini, Alessandro; Wein, Rosemary; Lapinski, Robert; Casal, David; Berkowitz, Richard L.

doi:10.1016/0002-9378(94)90043-4

Cited by 109 publications

(78 citation statements)

References 14 publications

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“…[12][13][14]23 Augmented IL-6 levels in amniotic fluid in combination with evaluation of cervical characteristics by vaginal sonography and elevated levels of fetal fibronectin in cervicovaginal secretions have proven to be the best method of predicting PTD. 24 Previous studies suggested that IL-6 derived from human decidual and chorion laeve cells 25,26 contribute to elevated amniotic fluid IL-6 levels involved in the pathophysiology of infectionrelated PTD.…”

Section: Discussionmentioning

confidence: 99%

“…8 Our laboratory recently demonstrated that decidual cells (DCs), the predominant endometrial cell type throughout pregnancy 9 are a major source of elevated maternal plasma IL-6 levels 10 implicated in inducing systemic endothelial cell activation and vascular damage in preeclampsia. 11 In the current study we hypothesized that DCs could also contribute to the well documented CAM-related increase in IL-6 levels in maternal plasma 12 as well as cervical and amniotic fluid IL-6 levels 13 that reliably predict PPROM and PTD 14 while acting as an autocrine/ paracrine modulator of the local immune cell population. To shed light on these questions, immunohistochemistry was used to localize IL-6 at the maternal-placental interface in DCs of placental sections from patients with CAM and gestational age-matched control placentas.…”

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confidence: 98%

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Regulation of Interleukin-6 Expression in Human Decidual Cells and Its Potential Role in Chorioamnionitis

Lockwood

Murk

Kayisli

et al. 2010

The American Journal of Pathology

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Chorioamnionitis frequently precedes both genital tract and placental inflammation and is both a primary cause of maternal morbidity and a major antecedent of preterm premature rupture of the membranes (PPROM) as well as preterm delivery (PTD). In most cases of chorioamnionitis , neutrophils dominate the decidua. In a subset of these cases , a predominance of monocytes is uniquely associated with both neonatal intraventricular hemorrhage and death. The multifunctional cytokine , interleukin-6 , promotes local monocyte dominance via several mechanisms. In this study , immunostaining of placental sections revealed significantly higher interleukin-6 HSCOREs in decidual cells (DCs) but not in interstitial trophoblasts , in chorioamnionitis versus gestational agematched control placentas (P < 0.05). In confluent leukocyte-free term DCs , secreted interleukin-6 levels in incubations with estradiol-17␤ were increased 2500-fold by IL-1␤ (P < 0.05). This up-regulation was inhibited by more than 50% in parallel incubations that included medroxyprogesterone acetate (n ‫؍‬ 12, P < 0.05). Western blotting data confirmed these enzyme-linked immunosorbent assay results; quantitative RT-PCR findings demonstrated corresponding changes in interleukin-6 mRNA levels. Specific inhibitors of signaling for both nuclear factor-B activation and p38-mitogen-activated protein kinase , but not for protein kinase C , significantly decreased IL-1␤-enhanced interleukin-6 expression levels in cultured DCs. In conclusion , in situ and in vitro results indicate that significantly enhanced interleukin-6 expression levels in DCs during chorioamnionitis could be pivotal in skewing decidual monocyte differentiation to macrophages.

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Section: Discussionmentioning

confidence: 99%

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confidence: 98%

Regulation of Interleukin-6 Expression in Human Decidual Cells and Its Potential Role in Chorioamnionitis

Lockwood

Murk

Kayisli

et al. 2010

The American Journal of Pathology

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“…Specimens with fFN concentrations Ͼ50 g/L are interpreted as positive. After fFN analysis, samples were stored at Ϫ70°C until cytokine measurements were performed (22 ). IL-6, TNF␣, and IL-2R were quantified on the DPC Immulite ® (Diagnostic Products Corporation) by use of sequential, two-site, solid-phase, chemiluminescent enzyme immunometric assays (23)(24)(25).…”

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confidence: 99%

Cervicovaginal Interleukin-6, Tumor Necrosis Factor-α, and Interleukin-2 Receptor as Markers of Preterm Delivery

et al. 2004

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et al. (16 ) and Noone et al. (14 ) found that the 5T allele is always in trans position with ⌬F508, producing male infertility, nonclassic CF, or a normal phenotype. Four of our samples (2.5%) carried only one mutation (⌬F508) without the 5T variant, but no homozygous 5T was detected. There were no homozygous carriers for ⌬F508 or R117H, or carriers of both ⌬F508 and R117H alleles. All of the patients carrying the 5T allele had been diagnosed with azoospermia; one of them had ductus deferens agenesis. For the rest of the cases. no such clinical data were available. The frequency of the 5T genotype (9%) in our patient population was higher than the frequency published (7 ) for the general European Caucasian population (5%).In summary, the proposed real-time PCR using hybridization probes coupled with melting curve analysis is a rapid and sensitive alternative to other known methods for detection of the CFTR 5T/7T/9T polymorphism.The control samples with different genotypes were generously provided by

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“…IL-6 is a key cytokine associated with adverse perinatal health outcomes [39][40][41][42][43][44]. Notably, IL-6 is secreted by adipose tissue [35].…”

Section: Discussionmentioning

confidence: 99%

33. Longitudinal changes in serum proinflammatory markers across pregnancy and postpartum: Effects of maternal body mass index

Christian

Porter

2014

Brain, Behavior, and Immunity

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Increased interleukin-6 concentrations in cervical secretions are associated with preterm delivery

Cited by 109 publications

References 14 publications

Regulation of Interleukin-6 Expression in Human Decidual Cells and Its Potential Role in Chorioamnionitis

Regulation of Interleukin-6 Expression in Human Decidual Cells and Its Potential Role in Chorioamnionitis

Cervicovaginal Interleukin-6, Tumor Necrosis Factor-α, and Interleukin-2 Receptor as Markers of Preterm Delivery

33. Longitudinal changes in serum proinflammatory markers across pregnancy and postpartum: Effects of maternal body mass index

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