Increased level of HLA-B27 expression in ankylosing spondylitis patients compared with healthy HLA-B27-positive subjects: a possible further susceptibility factor for the development of disease

Abstract

We found greater expression of HLA-B27 molecules in patients with AS than in healthy subjects. This phenomenon was not accompanied by general up-regulation of HLA class I molecules or by greater expression of classical T-cell activation markers. On this basis we propose that the higher expression of the HLA-B27 molecules is a further predisposing factor for the development of AS.

Two HLA–B27 alleles differently associated with spondylarthritis, B*2709 and B*2705, display similar intracellular trafficking and oligomer formation

“…Indeed, BRET values were significantly lowered by the C67S mutation in HLA-B*2705 (for fractions 5-10, P Ͻ 0.001; for fractions 11-17, P Ͻ 0.01) while BRET values appeared to be increased by the Y67C mutation in HLA-B*0702 (for fractions 5-8 and 13-16, P Ͻ 0.05). Nevertheless, BRET signaling observed with HLA-B*0702Y67C remained lower than that observed with HLA-B*2705 (for fractions 5-10, P Ͻ 0.01; for fractions [15][16][17] (Figures 4Ab-d). The results provided evidence that for each HLA-B subspecies, HC-10 labeling was much higher in the population that expressed high levels of HLA-B YFP than in the population that expressed lower levels.…”

“…Because the BRET technique is not well adapted to perform such studies in intact cells by microscopy, we performed BRET measurements on ER and plasma membrane fractions obtained by subcellular fractionation, using discontinuous sucrose gradients as described previously (31,32 , which is expressed at the plasma membrane, were used as controls for plasma membrane labeling and BRET signaling specificity. Calnexin and CD55 were used as markers for the ER (fractions 5-11) and the plasma membrane (fractions [13][14][15][16][17][18][19][20], respectively ( Figure 3A).…”

“…Indeed, only transgenic rats that carry a high HLA-B*2705 gene copy number develop a variety of pathologic effects closely resembling those associated with SpA (15). Moreover, the expression of HLA-B*2705 was shown to be increased on peripheral blood mononuclear cells from patients with SpA, as compared with healthy controls (16). Last, recent findings showed that the expression levels of ␤ 2 m might play an important role in the pathogenic effects of HLA-B27 (17).…”

The Stress Response and Its Functional Implications in the Immune Response After Surgery in Patients with Chronic Inflammation Undergoing Arthroplasty

“…The MHC complex includes the most polymorphic genomes in the human genomes with many consequences related to the transplantation and the autoimmune disease. It turned out that the human leukocyte antigen HLA-B27 is tightly correlated with the frequency of the spondylarthritis (Cauli et al, 2002;Jin & Wang, 2003). The adhesion molecules CD4+ and CD8+ are necessary to enhance the binding of the T cells and the APC.…”

HLA-B27 positivity: associated health implications

“…A diagnosis of HLA-B27 positivity is the commonest known aetiology associated with a number of systemic autoimmune inflammatory diseases (Jones, 2001;Cauli et al, 2002;Monnet et al, 2004;Levinson, 2005;Andrews and Lightman, 2008;Di Lorenzo, 2008;Cox et al, 2008. HLA-B27 positivity increases the likelihood of developing inflammatory bowel disease, Crohn's disease, ulcerative colitis, ankylosing spoindylitis, Reiter's syndrome, psoriasis and psoriatic arthropathy (Cauli et al, 2002;Di Lorenzo, 2008).…”