Increased levels of circulating Annexin A5 in Familial Mediterranean fever

Boyajyan, Anna; Mkrtchyan, G.; Hovhannisyan, Lilit; Hovsepyan, Tigran J

doi:10.1186/1476-9255-7-55

Cited by 6 publications

(5 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For locally advanced rectal cancer, an increased mRNA expression of AnxA5, but not AnxA4 was found in tumor tissue after radiochemotherapy [121]. Elevated serum AnxA5 level have been found under inflammatory conditions that are associated with higher IL-1 beta concentrations [122] and it will be of interest to elucidate whether tumor cells can secrete AnxA5 during therapy and thereby mediate their recognition by DCs and subsequent killing via CTLs. The modulation of inflammation and immunity by dying cells and the influence of the PS ligand AnxA5 has been considered in more detail elsewhere [112,123].…”

Section: Interference With Tumor Cell Clearance -Blocking Of Ps With mentioning

confidence: 98%

Induction of Abscopal Anti-Tumor Immunity and Immunogenic Tumor Cell Death by Ionizing Irradiation - Implications for Cancer Therapies

Frey¹,

Rubner²,

Wunderlich³

et al. 2012

CMC

126

View full text Add to dashboard Cite

Although cancer progression is primarily driven by the expansion of tumor cells, the tumor microenvironment and anti-tumor immunity also play important roles. Herein, we consider how tumors can become established by escaping immune surveillance and also how cancer cells can be rendered visible to the immune system by standard therapies such as radiotherapy or chemotherapy, either alone or in combination with additional immune stimulators. Although local radiotherapy results in DNA damage (targeted effects), it is also capable of inducing immunogenic forms of tumor cell death which are associated with a release of immune activating danger signals (non-targeted effects), such as necrosis. Necrotic tumor cells may result from continued exposure to death stimuli and/or an impaired phosphatidylserine (PS) dependent clearance of the dying tumor cells. In such circumstances, mature dendritic cells take up tumor antigen and mediate the induction of adaptive and innate anti-tumor immunity. Locally-triggered, systemic immune activation can also lead to a spontaneous regression of tumors or metastases that are outside the radiation field - an effect which is termed abscopal. Preclinical studies have demonstrated that combining radiotherapy with immune stimulation can induce anti-tumor immunity. Given that it takes time for immunity to develop following exposure to immunogenic tumor cells, we propose practical combination therapies that should be considered as a basis for future research and clinical practice. It is essential that radiation oncologists become more aware of the importance of the immune system to the success of cancer therapy.

show abstract

Section: Interference With Tumor Cell Clearance -Blocking Of Ps With mentioning

confidence: 98%

Induction of Abscopal Anti-Tumor Immunity and Immunogenic Tumor Cell Death by Ionizing Irradiation - Implications for Cancer Therapies

Frey¹,

Rubner²,

Wunderlich³

et al. 2012

CMC

126

View full text Add to dashboard Cite

show abstract

“…In contrast, an endogenous concentration of annexin V in BAL fluid obtained on day 15 was much lower and similarly detectable among treatment groups at 61-143 pg/ml (determined by ELISA, data not shown). Annexin V is an intracellular protein and its serum concentration was reported to range between 1.8 and 2.5 ng/ml in healthy subjects and 5.7 and 11 ng/ml in subjects with various diseases (4,23,36). No data is currently available about endogenous level of annexin V in human COPD.…”

Section: Inhibition Of Efferocytosis By Annexin Vmentioning

confidence: 98%

“…Furthermore, mutant annexin V, which lost binding affinity to PS, did not affect macrophage efferocytosis (19), clearly showing that an inhibitory effect of annexin V was PS-mediated. Notably, an inhibitory effect of annexin V on efferocytosis was concentration-dependent and a relatively higher concentration was needed to exert the effect in these studies, compared with a concentration present in human serum (4,23,36). The extracellular concentration of endogenous annexin V is presumably too low to affect macrophage efferocytosis.…”

mentioning

confidence: 95%

Annexin V decreases PS-mediated macrophage efferocytosis and deteriorates elastase-induced pulmonary emphysema in mice

Yoshida

Minematsu

Chubachi

et al. 2012

American Journal of Physiology-Lung Cellular and Molecular Phys

View full text Add to dashboard Cite

Efferocytosis is believed to be a key regulator for lung inflammation in chronic obstructive pulmonary disease. In this study we pharmacologically inhibited efferocytosis with annexin V and attempted to determine its impact on the progression of pulmonary emphysema in mouse. We first demonstrated in vitro and in vivo efferocytosis experiments using annexin V, an inhibitor for phosphatidylserine-mediated efferocytosis. We then inhibited efferocytosis in porcine pancreatic elastase (PPE)-treated mice. PPE-treated mice were instilled annexin V intranasally starting from day 8 until day 20. Mean linear intercept (Lm) was measured, and cell apoptosis was assessed in lung specimen obtained on day 21. Cell profile, apoptosis, and mRNA expression of matrix metalloproteinases (MMPs) and growth factors were evaluated in bronchoalveolar lavage (BAL) cells on day 15. Annexin V attenuated macrophage efferocytosis both in vitro and in vivo. PPE-treated mice had a significant higher Lm, and annexin V further increased that by 32%. More number of macrophages was found in BAL fluid in this group. Interestingly, cell apoptosis was not increased by annexin V treatment both in lung specimens and BAL fluid, but macrophages from mice treated with both PPE and annexin V expressed higher MMP-2 mRNA levels and had a trend for higher MMP-12 mRNA expression. mRNA expression of keratinocyte growth factor tended to be downregulated. We showed that inhibited efferocytosis with annexin V worsened elastase-induced pulmonary emphysema in mice, which was, at least partly, attributed to a lack of phenotypic change in macrophages toward anti-inflammatory one.

show abstract

“…6,10 Moreover, animal studies demonstrated diminished macrophages apoptosis in pyrin-truncation mice through an IL-1 independent pathway. 11,12 These results may reflect impaired apoptosis regulation in FMF-patients. However, there are some reports which do not support these results; Gomucio et al found that there is no difference between the effect of wild-type pyrin and mutated pyrin regarding both interaction with the ASC protein and effect on cell death.…”

Section: Discussionmentioning

confidence: 86%

“…Some studies reported that colchicine which could be inhibited apoptosis add to anti-inflammatory effect, reduced the surface expression of Fas. 12,15,16 However, there is no sufficient data about the effect of colchicine on apoptosis in the FMF patients. Boyajyan et al demonstrated less circulating annexin A5 levels which are an apoptotic marker in using regular colchicine patients.…”

Section: Discussionmentioning

confidence: 99%

Serum soluble fas ligand levels in familial Mediterranean fever

Çeri¹,

Unverdi²,

Şeneş³

et al. 2013

Renal Failure

View full text Add to dashboard Cite

Introduction: Fas/FasL system plays an important role in the regulation of cell life and death, and circulating levels of sFasL have been shown to increase in some inflammatory conditions. However, there is no sufficient information about the levels of sFasL in patients with FMF. This study was designed to evaluate the serum sFasL levels in patients with FMF during attack and attack-free periods. Methods: Twenty-five FMF patients in attack and forty-four in free-attack period, and 20 age-, sex-, and BMI-matched healthy controls were included in this study. Participants with any chronic diseases were excluded. Blood samples were obtained within the first 24 h of the attack period and between febrile attacks, and levels of WBC, ESR, Fibrinogen, hsCRP and sFasL were determined. Results: The levels of traditional acute phase reactants during the attack were significantly higher than the attack-free and controls (p50.05). The serum sFasL levels in the FMF study groups did not differ from the control group (0.70 AE 0.08 vs. 0.73 AE 0.12; 0.70 AE 0.08 vs. 0.83 AE 0.14; 0.73 AE 0.12 vs. 0.83 AE 0.14, respectively, p40.05). Moreover, the sFasL levels during the attack were not significantly different from those in attack-free patients (0.70 AE 0.08 vs. 0.83 AE 0.14, p40.05). Conclusion: In this study, we demonstrated that serum sFasL levels were not markedly affected in FMF and cannot be used as a supportive marker to differentiate attacks from attack-free periods. However, further studies are needed to determine its usefulness as a marker in clinical practice.

show abstract

Increased levels of circulating Annexin A5 in Familial Mediterranean fever

Cited by 6 publications

References 13 publications

Induction of Abscopal Anti-Tumor Immunity and Immunogenic Tumor Cell Death by Ionizing Irradiation - Implications for Cancer Therapies

Induction of Abscopal Anti-Tumor Immunity and Immunogenic Tumor Cell Death by Ionizing Irradiation - Implications for Cancer Therapies

Annexin V decreases PS-mediated macrophage efferocytosis and deteriorates elastase-induced pulmonary emphysema in mice

Serum soluble fas ligand levels in familial Mediterranean fever

Contact Info

Product

Resources

About