Increased levels of IgA antibodies against CRA and FRA recombinant antigens of Trypanosoma cruzi differentiate digestive forms of Chagas disease

Vasconcelos, Romero Henrique Teixeira; Amaral, Fábio N.; Cavalcanti, Maria G.A.M.; Silva, Edimilson D.; Ferreira, Antônio G. P.; Morais, Clarice Neuenschwander Lins de; Gomes, Yara M.

doi:10.1016/j.humimm.2010.07.004

Cited by 10 publications

(5 citation statements)

References 16 publications

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“…Thus, despite the fact that a few studies suggest an association of the IND form with a high production of anti-inflammatory and regulatory cytokines such as IL-4 and IL-10 and TGF-β, and of the CARD form with high levels of pro-inflammatory cytokines such as IFN-γ and TNF-α [29,30]) here we found results similar to other studies, where no relationship was observed between the production profile of a certain cytokine and Chagas disease clinical symptoms [17,20,31].…”

Section: Discussionsupporting

confidence: 90%

“…Several studies have used specific, specific antigens of T. cruzi, known as Cytoplasmatic Repetitive Antigen (CRA) and Flagellar Repetitive Antigen (FRA) [13,14] with aim to help clarifying the immune pathology of Chagas disease [15]. The role of CRA and FRA in the immune response of patients with chronic Chagas disease has been studied by our research group and promising results have been generated [16][17][18]. Here, the immune response of patients with chronic Chagas disease was assessed after in vitro stimulation of Peripheral Blood Mononuclear Cells (PBMCs) with CRA and FRA.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of Gene Expression of TGF-B and FOXP3 in Individuals Chronically Infected with Trypanosoma Cruzi

Lorena¹

2016

INID

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Chagas diseases till poses a serious endemic problem despite the fact that it was discovered over 100 years ago. The mechanisms that lead to the development of clinical manifestations may be attributed to the host immune response against the parasite. Individuals with the in Determinate (IND) form have regulatory mechanisms that limit pathology development. The aim of the present study was to assess the gene expression of TGF-β, a cytokine with an immuno regulatory profile, and FOXP3, a transcription factor, in patients with chronic Chagas disease after in vitro stimulation of Peripheral Blood Mononuclear Cells (PBMCs) with the recombinant antigens Cytoplasmatic Repetitive Antigen (CRA) and the Flagellar Repetitive Antigen (FRA) from Trypanosoma cruzi. We selected 37 patients with Chagas disease

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Evaluation of Gene Expression of TGF-B and FOXP3 in Individuals Chronically Infected with Trypanosoma Cruzi

Lorena¹

2016

INID

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“…It differs from its counterparts in mammalian cells in several structural, biochemical and immunological respects, suggesting that its components may be potential targets for the development of new anti-parasitic drugs. Several T. cruzi flagellar and cytoskeletal proteins are potent immunogens in humans and have been used as specific diagnostic and prognostic antigens in the serodiagnosis of Chagas' disease [1], [2], [3]. Immunization of mice with purified or semi-purified fractions of T. cruzi cytoskeleton induced high levels of specific humoral and cellular immune responses that protected the mice against a fatal challenge [4], [5], [6].…”

Section: Introductionmentioning

confidence: 99%

The Repetitive Cytoskeletal Protein H49 of Trypanosoma cruzi Is a Calpain-Like Protein Located at the Flagellum Attachment Zone

Galetovic

Souza²,

Santos³

et al. 2011

PLoS ONE

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Background Trypanosoma cruzi has a single flagellum attached to the cell body by a network of specialized cytoskeletal and membranous connections called the flagellum attachment zone. Previously, we isolated a DNA fragment (clone H49) which encodes tandemly arranged repeats of 68 amino acids associated with a high molecular weight cytoskeletal protein. In the current study, the genomic complexity of H49 and its relationships to the T. cruzi calpain-like cysteine peptidase family, comprising active calpains and calpain-like proteins, is addressed. Immunofluorescence analysis and biochemical fractionation were used to demonstrate the cellular location of H49 proteins.Methods and FindingsAll of H49 repeats are associated with calpain-like sequences. Sequence analysis demonstrated that this protein, now termed H49/calpain, consists of an amino-terminal catalytic cysteine protease domain II, followed by a large region of 68-amino acid repeats tandemly arranged and a carboxy-terminal segment carrying the protease domains II and III. The H49/calpains can be classified as calpain-like proteins as the cysteine protease catalytic triad has been partially conserved in these proteins. The H49/calpains repeats share less than 60% identity with other calpain-like proteins in Leishmania and T. brucei, and there is no immunological cross reaction among them. It is suggested that the expansion of H49/calpain repeats only occurred in T. cruzi after separation of a T. cruzi ancestor from other trypanosomatid lineages. Immunofluorescence and immunoblotting experiments demonstrated that H49/calpain is located along the flagellum attachment zone adjacent to the cell body.ConclusionsH49/calpain contains large central region composed of 68-amino acid repeats tandemly arranged. They can be classified as calpain-like proteins as the cysteine protease catalytic triad is partially conserved in these proteins. H49/calpains could have a structural role, namely that of ensuring that the cell body remains attached to the flagellum by connecting the subpellicular microtubule array to it.

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“…In previous works of our research group, the IgG2 isotype against the FRA antigen was associated with the CARD form [14] and the IgA isotype against both antigens was associated with the DIG and CD forms [15]. In the present work we continue to study the humoral immune response in chronic Chagas disease, evaluating IgM serum levels against the CRA and FRA recombinant antigens of T. cruzi.…”

Section: Discussionmentioning

confidence: 58%

“…Recently, Vasconcelos et al [15] studied the serum levels of IgA antibodies against these recombinant antigens and demonstrated that the digestive forms of the disease present high levels of this isotype of immunoglobulin.…”

Section: Introductionmentioning

confidence: 99%

Immunoglobulin M antibodies against CRA and FRA recombinant antigens of Trypanosoma cruzi in chronic chagasic patients

et al. 2011

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Increased levels of IgA antibodies against CRA and FRA recombinant antigens of Trypanosoma cruzi differentiate digestive forms of Chagas disease

Cited by 10 publications

References 16 publications

Evaluation of Gene Expression of TGF-B and FOXP3 in Individuals Chronically Infected with Trypanosoma Cruzi

Evaluation of Gene Expression of TGF-B and FOXP3 in Individuals Chronically Infected with Trypanosoma Cruzi

The Repetitive Cytoskeletal Protein H49 of Trypanosoma cruzi Is a Calpain-Like Protein Located at the Flagellum Attachment Zone

Immunoglobulin M antibodies against CRA and FRA recombinant antigens of Trypanosoma cruzi in chronic chagasic patients

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