2005
DOI: 10.1002/art.21130
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Increased levels of serum protein oxidation and correlation with disease activity in systemic lupus erythematosus

Abstract: Objective. To examine protein oxidation in systemic lupus erythematosus (SLE) and to correlate levels of protein oxidation products with disease activity.Methods. Serum was collected from SLE patients and healthy control subjects. Protein-bound carbonyls and the pro-oxidant enzyme myeloperoxidase (MPO) were quantified by enzyme-linked immunosorbent assay. Protein thiols were quantified using 5,5-dithionitrobenzoic acid. Protein-bound amino acids and methionine, tyrosine, and phenylalanine oxidation products we… Show more

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Cited by 121 publications
(112 citation statements)
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“…indicating decreased blood sulfhydryl or GSH concentrations in T-cells (10) of patients with SLE (9). They also agree with literature data suggesting a defective antioxidant system in MRL/lpr mice (19).…”
Section: Discussionsupporting
confidence: 89%
See 1 more Smart Citation
“…indicating decreased blood sulfhydryl or GSH concentrations in T-cells (10) of patients with SLE (9). They also agree with literature data suggesting a defective antioxidant system in MRL/lpr mice (19).…”
Section: Discussionsupporting
confidence: 89%
“…Oxidative stress has also been implicated in the pathogenesis of several degenerative diseases, such as Alzheimer disease, Parkinson disease, systemic lupus erythematosus, and cancer. In particular, its involvement in autoimmune disease has been supported by the increase of oxidative stress markers (9,10) and by the preventive effect played by the dietary intake of antioxidants (11).…”
Section: Introductionmentioning
confidence: 99%
“…Thiol oxidation analysis was quantified spectrophotometrically using the DTNB method described by Philip E. Morgan et al with some improvements (Morgan et al, 2005). Protein samples were extracted from hippocampal slices of adult or aging rats.…”
Section: Biochemical Analysismentioning
confidence: 99%
“…Amino acid analysis was performed by HPLC analysis using OPA derivatisation after delipidation and methanesulphonic acid hydrolysis [26]. LDL samples (250 μg protein) were prepared for hydrolysis by addition of sodium borohydride (10 μl, 10 mg/ml), deoxycholic acid (50 μl, 0.3% w/v) and trichloroacetic acid (100 μl, 50% w/v), followed by centrifugation at 4,300 g for 2 min.…”
Section: Characterisation Of Ldl Modificationsmentioning
confidence: 99%