Increased Levels of the Long Intergenic Non–Protein Coding RNA POU3F3 Promote DNA Methylation in Esophageal Squamous Cell Carcinoma Cells

Li, Wei; Zheng, Jian; Deng, Jieqiong; You, Yonghe; Wu, Hongchun; Li, Na; Lü, Jiachun; Zhou, Yifeng

doi:10.1053/j.gastro.2014.03.002

Cited by 154 publications

(127 citation statements)

References 43 publications

Supporting

Mentioning

120

Contrasting

Order By: Relevance

“…Since lncRNA AFAP1-AS1 was found to be highly expressed in esophageal adenocarcinoma and silencing its expression significantly inhibited the proliferation, invasion and metastasis of esophageal adenocarcinoma cell lines (17), the research of lncRNAs and ESCC has attracted the attention of many scholars, and a few types of lncRNAs have been identified in ESCC. For example, lncRNA AFAP1-AS1 (9), HOTAIR (10) and POU3F3 (18) are highly expressed in ESCC, and their high expression can promote the development of ESCC and adversely affect the prognosis. Following knockdown of these lncRNAs, the proliferation, invasion and migration ability of ESCC cells was significantly inhibited.…”

Section: Discussionmentioning

confidence: 99%

LncRNA‑ECM is overexpressed in esophageal squamous cell carcinoma and promotes tumor metastasis

Yao

Shen

et al. 2018

Oncol Lett

View full text Add to dashboard Cite

Abstract. The aim of the present study was to investigate the expression of long non-coding(lnc) RNA-extracellular matrix (ECM) in esophageal squamous cell carcinoma (ESCC) and its effect on ESCC metastasis. Using reverse transcription-quantitative polymerase chain reaction (RT-qPCR), the expression of lncRNA-ECM in ESCC tissues was investigated and compared with that in corresponding adjacent tissues. In addition, the expression of lncRNA-ECM in the human ESCC cell lines TE-1, EC9706, KYSE150, Eca109 and KYSE30 was also detected and compared with that in the normal esophageal mucosal epithelial cell line HET-1A. The clinicopathological association between lncRNA-ECM and ESCC was assessed. Silencing and overexpression of lncRNA-ECM in ESCC TE-1 and Eca109 cells determined the correlation between lncRNA-ECM expression and ESCC invasion and metastasis. The possible target genes of lncRNA-ECM were predicted and verified by bioinformatics analysis and experimental results. The expression level of intercellular adhesion molecule 1 (ICAM1) was detected in ESCC tissues by RT-qPCR and the correlation between the expression of ICAM1 and lncRNA-ECM was analyzed. Changes in the expression of ICAM1 in ESCC TE-1 and Eca109 cell lines were evaluated after knocking down lncRNA-ECM and transfection of lncRNA-ECM overexpression plasmids. The expression level of lncRNA-ECM in the tissues of ESCC with lymph node metastasis were significantly increased compared with ESCC with no lymph metastasis (P<0.05). LncRNA-ECM silencing notably reduced the invasion and metastasis of TE-1 and Eca109 cells, while lncRNA-ECM overexpression promoted the invasion and metastasis of the two cell lines. The expression level of ICAMI was directly correlated with the expression of lncRNA-ECM, suggesting that ICAM1 may be the downstream target gene of lncRNA-ECM. LncRNA-ECM was revealed as being overexpressed in ESCC. LncRNA-ECM expression was positively correlated with metastasis and may affect the metastasis of ESCC through ICAMI regulation. These findings indicate that lncRNA-ECM may be promising as a novel biomarker for the diagnosis and prediction of prognosis for ESCC, and it may also serve as a novel therapeutic target for ESCC. IntroductionEsophageal cancer is one of the most common malignancies, with an incidence rate that ranks sixth in China and fourth among the causes of cancer-related mortality (1). Esophageal cancer is also one of the most common lethal tumors worldwide with the sixth highest mortality rate (2). Esophageal squamous cell carcinoma (ESCC) accounts for >90% of the cases of esophageal cancer, and has a high degree of aggressiveness and complexity of biological behavior, making its clinical course variable and heterogeneous. A considerable number of ESCC patients have already metastasized at diagnosis. The metastasis of ESCC is the main cause of poor prognosis and mainly involves lymph node and distant metastasis (3). Previous studies demonstrated that the 5-year survival rate of ESCC patients without metastasis was 70-92%, whe...

show abstract

Section: Discussionmentioning

confidence: 99%

LncRNA‑ECM is overexpressed in esophageal squamous cell carcinoma and promotes tumor metastasis

Yao

Shen

et al. 2018

Oncol Lett

View full text Add to dashboard Cite

show abstract

“…Although the function of most lncRNAs are still unknown, the numbers and accumulating evidence involved in cancers are increasing. As for long intergenic non-protein coding RNA, only several studies has reported in NSCLC [28,29] , let alone the rs2665675 of LINC01495. Meanwhile, TRAPPC9 impact the development of cancer because it regulates the trans-Golgi exit process which is essential for cancer development.…”

Section: Discussionmentioning

confidence: 99%

A Case-Control Study of the Relationship between LINC01495, TRAPPC9 Polymorphisms and Lung Cancer Susceptibility in Northeast Chinese Population

Yang¹,

Ren²,

Qu³

et al. 2018

International Journal of Research in Environmental Science

View full text Add to dashboard Cite

“…The CpG islands near the POU3F3 gene are highly methylated in glioma and other cancers. Li W et al have demonstrated that linc‐POU3F3 physically interacts with EZH2, bringing DNMTs to the promoter region in oesophageal squamous cell carcinoma 95. Thereby, this evolutionarily conserved linc‐RNA epigenetically modulates neighbouring gene expression, which could be its common mechanism of action in cancers.…”

Section: Epigenetic Alterations Induced By Lncrnasmentioning

confidence: 99%

Long non‐coding RNAs in brain tumours: Focus on recent epigenetic findings in glioma

Pop

Enciu

Necula

et al. 2018

J Cellular Molecular Medi

View full text Add to dashboard Cite

Glioma biology is a major focus in tumour research, primarily due to the aggressiveness and high mortality rate of its most aggressive form, glioblastoma. Progress in understanding the molecular mechanisms behind poor prognosis of glioblastoma, regardless of treatment approaches, has changed the classification of brain tumours after nearly 100 years of relying on anatomopathological criteria. Expanding knowledge in genetic, epigenetic and translational medicine is also beginning to contribute to further elucidating molecular dysregulation in glioma. Long non‐coding RNAs (lncRNAs) and their main representatives, large intergenic non‐coding RNAs (lincRNAs), have recently been under scrutiny in glioma research, revealing novel mechanisms of pathogenesis and reinforcing others. Among those confirmed was the reactivation of events significant for foetal brain development and neuronal commitment. Novel mechanisms of tumour suppression and activation of stem‐like behaviour in tumour cells have also been examined. Interestingly, these processes involve lncRNAs that are present both during normal brain development and in brain malignancies and their reactivation might be explained by epigenetic mechanisms, which we discuss in detail in the present review. In addition, the review discusses the lncRNAs‐induced changes, as well as epigenetic changes that are consequential for tumour formation, affecting, in turn, the expression of various types of lncRNAs.

show abstract

Increased Levels of the Long Intergenic Non–Protein Coding RNA POU3F3 Promote DNA Methylation in Esophageal Squamous Cell Carcinoma Cells

Cited by 154 publications

References 43 publications

LncRNA‑ECM is overexpressed in esophageal squamous cell carcinoma and promotes tumor metastasis

LncRNA‑ECM is overexpressed in esophageal squamous cell carcinoma and promotes tumor metastasis

A Case-Control Study of the Relationship between LINC01495, TRAPPC9 Polymorphisms and Lung Cancer Susceptibility in Northeast Chinese Population

Long non‐coding RNAs in brain tumours: Focus on recent epigenetic findings in glioma

Contact Info

Product

Resources

About