Increased levels of tumor necrosis factor and interleukin 1 in bronchoalveolar lavage fluids from pigs infected with Mycoplasma hyopneumoniae

Asai, Tetsuo; Okada, Mizue; Ono, Masaaki; Irisawa, Toshio; Mori, Yoshihide; Yokomizo, Yuuichi; Sato, Sizuo

doi:10.1016/0165-2427(93)90085-i

Cited by 91 publications

(58 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…IL-6 is a second wave cytokine, and it has been reported that serum levels of IL-6 increased in lung cancer patients in relation to the intensity of the acute phase response (Yanagawa et al, 1995). Previous studies showed that TNF-α and IL-6 were produced in the airway or BALF during mycoplasmal infection in pigs, and lung inflammation was observed when the production of these cytokines was increased (Asai et al, 1993, Thacker et al, 2000. Therefore, in this study, the levels of inflammatory cytokines, including IL-6 and TNF-α, were determined in BALF to examine the inflammatory response to ZnO-NP exposure (Fig.…”

Section: Effect Of Zno-nps On Balf Biochemical Parametersmentioning

confidence: 99%

Evaluation of Recovery From Acute Lung Injury Induced by Intratracheal Instillation of Zinc Oxide Nanoparticles

Yoo¹

2018

Appl. Ecol. Env. Res.

View full text Add to dashboard Cite

Abstract. Zinc oxide nanoparticles (ZnO-NPs) can potentially cause adverse health effects because they are highly soluble and widely used. We evaluated the effect of ZnO-NPs in rat lung. ZnO-NPs were administered via intratracheal instillation to Sprague Dawley rats at three different dosages (1, 5, and 10 mg/kg body weight). Macrophage numbers, total cell count, and total protein concentrations in bronchoalveolar lavage fluid (BALF) highly increased at day 1 in a dose-dependent manner relative to those in the control. Macrophage numbers and total protein concentrations were still elevated at day 14 relative to baseline, whereas total cell number continued to increase. These results indicate that inflammation had not fully resolved by day 14 in rat lungs. Furthermore, a significant increase in IL-6 (but not TNF-α) was observed in 5 and 10 mg/kg dose groups at day 1, which was likely caused by a ZnO-NP-treatment-induced increase in polymorphonuclear leukocytes. Histological examination indicated that multifocal acute inflammation occurred in the ZnO-NP-treated groups and progressed to chronic inflammation by day 14. A partial recovery of lung injury was observed within 14 days after exposure. In conclusion, the results of this study were consistent with those of previous sub-chronic exposure studies.

show abstract

Section: Effect Of Zno-nps On Balf Biochemical Parametersmentioning

confidence: 99%

Evaluation of Recovery From Acute Lung Injury Induced by Intratracheal Instillation of Zinc Oxide Nanoparticles

Yoo¹

2018

Appl. Ecol. Env. Res.

View full text Add to dashboard Cite

show abstract

“…Bioassay for cytokines: TNF-α in sera was assayed on the basis of cytolytic activity against WEHI 164 clone 28-4 cells using the 3-2,5-diphenyltetrazolium bromide (MTT) method as described previously [3]. Recombinant human (rh) TNF-α was used as a standard to calculate bovine TNF-α activity.…”

Section: Methodsmentioning

confidence: 99%

“…Endotoxemia was confirmed in all but one in the present study. Endotoxin, which is a structural component in cell wall of Gram-negative bacteria such as Salmonella [12,18,19,22,24], elicits a variety of reactions through the induction of proinflammatory cytokines such as TNF-α, IL-1 and IL-6 [3,13,18,19,22,25]. TNF-α, in particular, plays a critical role in the pathogenesis of septic shock syndrome induced by endotoxemia [2,18].…”

Section: Facs Analysis Of Pbmcsmentioning

confidence: 99%

Inflammatory Cytokines and Antigen-Responsive Mononuclear Cells in Peripheral Blood of Cattle Infected with Salmonella Takoradi.

Konnai

Htsuka

Sakamoto³

et al. 2001

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRACT. To determine the immunological response in lactating dairy cows infected with Salmonella (S.) Takoradi, the relationships among distributions of peripheral blood mononuclear cell (PBMC) subpopulations, endotoxin concentrations and dynamics of inflammatory cytokines in blood were investigated. The ratio of CD4 + T cells to CD8 + T cells was significantly lower in the affected cattle than in the control cattle (p<0.05) to decrease in the number of CD4 + T cells in the infected cattle. In contrast, the numbers of γδ-T cells, MHC class II-positive cells were significantly higher in the affected cattle than in the control cattle (p<0.01 respectively). Endotoxemia was found in all but one of the affected cattle. Serum IL-1 and IL-6 bioactivities were significantly higher in the affected cattle than in the control cattle (IL-1, p<0.05; IL-6, p<0.01). Serum TNF-α activities and levels were not detected in the control and affected cattle. The activities of proinflammatory cytokines determined by the bioassay are important to the relationships between concentration of endotoxin, cytokines and clinical signs, such as leukocytosis, leukopenia, fever or bacterial shedding. Serum IL-2 levels were lower in the affected cattle than in the control cattle. Serum IFN-γ was not detected in the affected cattle except one. These results by the ELISA seemed to reflect the condition of subpopulation in the PBMCs from the shedding cattle. The present results suggest that cellular immunity is suppressed while the humoral immunity is activated in acute bovine salmonellosis. KEY WORDS: bovine salmonellosis, cytokine, endotoxin, PBMC.

show abstract

“…In vivo elicitation of proinflammatory cytokines, such as TNF-␣, IL-1␤, and IL-6 occurred following infection of pigs with M. hyopneumoniae (14) and inoculation of mice with either M. pneumoniae (336) or with M. pulmonis (122,318). In addition, mRNAs encoding GM-CSF (336) and the up-regulating cytokine IFN-␥ were detected in lung tissues and in cells present in bronchoalveolar lavage fluid from animals exposed to M. pneumoniae or M. pulmonis (122,307,318,336).…”

Section: Proinflammatory Cytokinesmentioning

confidence: 99%

Molecular Biology and Pathogenicity of Mycoplasmas

2002

View full text Add to dashboard Cite

Increased levels of tumor necrosis factor and interleukin 1 in bronchoalveolar lavage fluids from pigs infected with Mycoplasma hyopneumoniae

Cited by 91 publications

References 15 publications

Evaluation of Recovery From Acute Lung Injury Induced by Intratracheal Instillation of Zinc Oxide Nanoparticles

Evaluation of Recovery From Acute Lung Injury Induced by Intratracheal Instillation of Zinc Oxide Nanoparticles

Inflammatory Cytokines and Antigen-Responsive Mononuclear Cells in Peripheral Blood of Cattle Infected with Salmonella Takoradi.

Molecular Biology and Pathogenicity of Mycoplasmas

Contact Info

Product

Resources

About