Increased native chemiluminescence in granulocytes isolated from synovial fluid and peripheral blood of patients with rheumatoid arthritis

Abstract

Polymorphonuclear leukocytes (PMNs) isolated from peripheral blood and synovial fluid of patients with rheumatoid arthritis and from peripheral blood of volunteers were stimulated with 12-phorbol-13-myristate acetate (PMA). No significant differences in luminol-amplified chemiluminescence were found between different patients and control groups. However, two distinct patterns of native chemiluminescence were observed. Type I showed no, or only a small, increase in native chemiluminescence with integral counts over 30 min less than 3 x 10(5) cpm, and the majority of samples from volunteers were of this type. Type II was characterized by a burst of native chemiluminescence starting 8 to 15 min after cell stimulation. It was found in most PMN samples from patients with rheumatoid arthritis. Integral counts over 30 min were always higher than 10(6) cpm and as high as 10(8) cpm in some cases. A strong inhibition of the Type II native chemiluminescence was caused by desferal, catalase, thiourea, and glutathione. However, the luminol-amplified chemiluminescence remained unchanged or was only slightly decreased under the same experimental conditions. Sodium azide strongly inhibited both kinds of luminescence. Hydroxyl radicals, formed in a Fenton reaction, may be important intermediates in the Type II native chemiluminescence.

Reduction of oxidative stress in adjuvant arthritis. Comparison of efficacy of two pyridoindoles: stobadine dipalmitate and SMe1.2HCl.

“…ROS and RNS are of great relevance in inflammatory and immune processes involved in the pathogenesis of RA (Cross et al, 2006). There are multiple experimental studies dedicated to neutrophil-generated chemiluminescence of whole blood (Arnhold et al, 1994;Miesel et al, 1996;Cedergren et al, 2007) and of synovial fluid (Arnhold et al, 1994;Cedergren et al, 2007), depending on the disease severity in patients with RA. An increase in whole blood chemiluminescence (2-8 times) was shown in RA patients compared with healthy volunteers (Miesel et al, 1996).…”

Modern Pharmacological Approaches to Therapies: Substances Tested in Animal Models of Rheumatoid Arthritis

“…Recent evidence from animal models of RA emphasizes the importance of neutrophils in the initiation and progression of AA (Cross et al, 2006). There are multiple experimental studies dedicated to neutrophil-generated chemiluminescence of whole blood (Arnhold et al, 1994;Cedergren et al, 2007; and of synovial fluid (Arnhold et al, 1994;Cedergren et al, 2007), depending on the disease severity in patients with RA. An increase in whole blood chemiluminescence (2-8 fold) was shown in RA patients compared with healthy volunteers .…”

IR and NMR Studies on the Action of Hypochlorous Acid on Chondroitin Sulfate and Taurine

“…The predominant role of myeloperoxidase and its product, hypochlorous acid, in cartilage degradation is clearly reflected by two different experimental results: On the one hand, neutrophils isolated from synovial fluids of patients with rheumatoid arthritis are characterized by a markedly increased native chemiluminescence (9), which correlates well with MPO activity, i.e., with HOCl concentration (9). On the other hand, cell-free synovial fluids from patients with rheumatoid arthritis show intense 1 H NMR signals arising from degradation products of cartilage (oligosaccharides with N-acetyl groups and acetate).…”