Increased numbers of FoxP3‐expressing <scp>CD</scp>4<sup>+</sup> <scp>CD</scp>25<sup>+</sup> regulatory T cells in peripheral blood from dogs with atopic dermatitis and its correlation with disease severity

Hauck, Verena; Hügli, Patrick; Meli, Marina L.; Rostaher, Ana; Fischer, Nina; Hofmann‐Lehmann, Regina; Favrot, Claude

doi:10.1111/vde.12279

Cited by 26 publications

(48 citation statements)

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“…It was demonstrated that in dogs CD4 + CD25 + Foxp3 + cells were able to suppress the proliferation of responder CD4 + T cells in vitro [27]. Our study demonstrated that the percentage of circulating CD4 + CD25 + Foxp3 + cells in atopic dogs was significantly higher compared to healthy dogs, and these results were similar to recent data of other research groups [28, 29]. On the contrary other investigations have indicated that the Treg cells are not fully efficient in atopic patients in comparison with healthy individuals.…”

Section: Discussionsupporting

confidence: 92%

“…In vitro studies on T lymphocytes isolated from human grass allergic donors showed that Treg cells from these donors failed to inhibit proliferation but not cytokine production of CD4 + CD25 − T cells at high antigen doses, while Treg from non-atopic donors retained their regulatory properties [30]. It is possible, however, that the higher percentage of Tregs detected in AD patients in our study could be related to the chronic nature of the disease [28]. …”

Section: Discussionmentioning

confidence: 76%

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Lymphocytic, cytokine and transcriptomic profiles in peripheral blood of dogs with atopic dermatitis

et al. 2016

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BackgroundCanine atopic dermatitis (cAD) is a common chronic and pruritic skin disease in dogs. The development of cAD involves complex interactions between environmental antigens, genetic predisposition and a number of disparate cell types. The aim of the present study was to perform comprehensive analyses of peripheral blood of AD dogs in relation to healthy subjects in order to determine the changes which would be characteristic for cAD.ResultsThe number of cells in specific subpopulations of lymphocytes was analyzed by flow cytometry, concentration of chosen pro- and anti-inflammatory cytokines (IL-4, IL-10, IL-13, TNF-α, TGF-β1) was determined by ELISA; and microarray analysis was performed on RNA samples isolated from peripheral blood nuclear cells of AD and healthy dogs. The number of Th cells (CD3+CD4+) in AD and healthy dogs was similar, whereas the percentage of Tc (CD3+CD8+) and Treg (CD4+CD25+ Foxp3+) cells increased significantly in AD dogs. Increased concentrations of IL-13 and TNF-α, and decreased levels of IL-10 and TGF-β1 was observed in AD dogs. The level of IL-4 was similar in both groups of animals. Results of the microarray experiment revealed differentially expressed genes involved in transcriptional regulation (e.g., transcription factors: SMAD2, RORA) or signal transduction pathways (e.g., VEGF, SHB21, PROC) taking part in T lymphocytes lineages differentiation and cytokines synthesis.ConclusionsResults obtained indicate that CD8+ T cells, beside CD4+ T lymphocytes, contribute to the development of the allergic response. Increased IL-13 concentration in AD dogs suggests that this cytokine may play more important role than IL-4 in mediating changes induced by allergic inflammation. Furthermore, observed increase in Treg cells in parallel with high concentrations of TNF-α and low levels of IL-10 and TGF-β1 in the peripheral blood of AD dogs point at the functional insufficiency of Treg cells in patients with AD.Electronic supplementary materialThe online version of this article (doi:10.1186/s12917-016-0805-6) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 76%

Lymphocytic, cytokine and transcriptomic profiles in peripheral blood of dogs with atopic dermatitis

et al. 2016

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“…Six ml of whole blood was obtained from the jugular vein with a 10 ml syringe and a 22 gauge 30 mm needle and dispensed into tubes containing ethylenediaminetetraacetic acid (EDTA). The blood was collected in the morning from fasted animals and was kept at room temperature (RT) on a roller for 4 h before PBMC isolation by Ficoll (Histopaque ® 1.077, Sigma‐Aldrich; Buchs, Switzerland) density gradient separation, as reported previously . The PBMCs were counted with a haemocytometer and two aliquots were prepared, one for immediate analysis by flow cytometry analysis (0.5–1 × 10 6 cells) and one for cryopreservation (1–3 × 10 6 cells).…”

Section: Methodssupporting

confidence: 49%

“…Regulatory T (Treg) cells have gained considerable attention in recent years because they seem to be important diagnostic and therapeutic indicators in various immunological conditions, such as autoimmune, neoplastic, infectious and allergic diseases . This is relevant to the field of dermatology where Treg cells are involved in allergic reactions and wound healing .…”

Section: Introductionmentioning

confidence: 99%

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The effects of cryopreservation on the expression of canine regulatory T‐cell markers

Tarpataki

Wawrzyniak²,

Akdiş³

et al. 2017

Veterinary Dermatology

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Interleukin 10 and transforming growth factor‐beta 1 plasma levels in atopic dogs before and during immunotherapy

et al. 2021

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Background Human studies suggest that the cytokines, interleukin 10 (IL‐10) and transforming growth factor‐beta 1 (TGF‐ß1) may play an important role in allergen‐specific immunotherapy (ASIT). However, there is little known about the function of these cytokines in atopic dogs. This study compared the plasma levels of IL‐10 and TGF‐ß1 in atopic and control dogs and investigated their changes during different ASIT approaches. Methods A total of 54 atopic and 32 control dogs were included. Immunotherapy was performed in 30 atopic dogs. The dogs undergoing immunotherapy were allocated to four groups of different ASIT approaches (namely subcutaneous, intralymphatic, sublingual ASIT and subcutaneous ASIT with recombinant allergens). Blood samples were collected at four timepoints throughout the one year of ASIT. Canine atopic dermatitis extent and severity index, pruritus visual analogue scale and medication score were recorded at each timepoint. Commercially available ELISA kits were used to quantify IL‐10 and TGF‐ß1 in plasma. Results There was no significant difference in IL‐10 and TGF‐ß1 between atopic and control dogs. The IL‐10 levels were significantly increased in the intralymphatic group at the end of the study. No significant differences were found in the other groups for both IL‐10 and TGF‐ß1. Conclusion The findings of this work suggest that IL‐10 and TGF‐ß1 cannot be used to monitor the course of the disease during ASIT.

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Increased numbers of FoxP3‐expressing CD4⁺ CD25⁺ regulatory T cells in peripheral blood from dogs with atopic dermatitis and its correlation with disease severity

Cited by 26 publications

References 42 publications

Lymphocytic, cytokine and transcriptomic profiles in peripheral blood of dogs with atopic dermatitis

Lymphocytic, cytokine and transcriptomic profiles in peripheral blood of dogs with atopic dermatitis

The effects of cryopreservation on the expression of canine regulatory T‐cell markers

Interleukin 10 and transforming growth factor‐beta 1 plasma levels in atopic dogs before and during immunotherapy

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Increased numbers of FoxP3‐expressing CD4+ CD25+ regulatory T cells in peripheral blood from dogs with atopic dermatitis and its correlation with disease severity

Cited by 26 publications

References 42 publications

Lymphocytic, cytokine and transcriptomic profiles in peripheral blood of dogs with atopic dermatitis

Lymphocytic, cytokine and transcriptomic profiles in peripheral blood of dogs with atopic dermatitis

The effects of cryopreservation on the expression of canine regulatory T‐cell markers

Interleukin 10 and transforming growth factor‐beta 1 plasma levels in atopic dogs before and during immunotherapy

Contact Info

Product

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Increased numbers of FoxP3‐expressing CD4⁺ CD25⁺ regulatory T cells in peripheral blood from dogs with atopic dermatitis and its correlation with disease severity